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The map label for this gene is htrB [H]

Identifier: 77457923

GI number: 77457923

Start: 1892614

End: 1893546

Strand: Direct

Name: htrB [H]

Synonym: Pfl01_1696

Alternate gene names: 77457923

Gene position: 1892614-1893546 (Clockwise)

Preceding gene: 77457914

Following gene: 77457924

Centisome position: 29.4

GC content: 61.52

Gene sequence:

>933_bases
ATGGATCGCCCGCGTTTTCGAAAAGCATTTCTTGCCCCACGCTTCTGGCCGCTCTGGTGCGGCCTCGGGCTGTTGTGGCT
GATCGTGCAGTTGCCTTATCCGGCATTGCTCGCCATCGGCCGTTTTCTCGGTGCCCTGATGTATCGCTTCGCCGGCGACC
GACGGCGCATCGCCAAGCGCAATCTGGAACTGTGTTTCCCGGAAAAGTCCGCCGCCGAACGCAAGCGCCTGCTCAAGGAA
AACTTTGCCTCCACCGGCATCGCCTTCTTTGAAATGGCCATGAGCTGGTGGTGGTCGCGTCAGCGGCTGGCGAAACTGGC
TCACGTCGAAGGCCTGGAGCATCTGCAAAAGGCCCAGCGTGAAGGCAAGGGCGTGATTCTGATGGCGGTGCATTTCACCA
CCCTGGAAATCGGCGCGGCGCTGCTCGGCCAGCAGCACACCATCGACGGCATGTACCGCGAACACAAGAACCCGCTGTTC
GACTTCGTCCAGCGTCAGGGCCGCGAGCGGCACAATCTCGACTCGCTGGCGGTGGAACGTGACGACGTGCGCGGCATGCT
CAAGTTGCTGCGCGCAGGCCGGGCGATCTGGTATGCACCGGATCAGGACTACGGCGCCAAGCAAAGTATCTTTGTGCCGC
TGTTCGGTATTCAGGCCGCAACGGTCACTGCCACCAGCAAGTTCGCCCGGCTGGGCAAGGCGCTGGTGGTGCCGTTCACC
CAGGAACGTCTGGCTGACGGCAGCGGTTATCGTCTGGTGATTCATCCGCCGCTGGAAGGTTTCCCGGGCGAGACCGAAGA
AGAAGATTGCATCCGCATCAATCAGTGGGTCGAAGGCGTGTTGCGTGAGTGTCCCGAGCAATACTTGTGGGCACATCGGC
GCTTCAAGAGTCGTCCGCCAGGCGAGCCGAAGCTGTACGCCAAACGCGGTTGA

Upstream 100 bases:

>100_bases
TGTCACTAAAAGTGCCGAGACTTATAACGAGAACGCCCGCAGGCCGCAAGCCGGGTCGAACGATGTAGAATGCGCGGCCA
CTGTATTTACGGAAGCTTTA

Downstream 100 bases:

>100_bases
TTGACCCATTACCATAAGCACCACGGAGCGTTGCGATGAGCCCAGCTGAACCGGTCACAGGTTTGATTCTTTCCGGCGGC
GGGGCTCGGGCGGCGTATCA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDRPRFRKAFLAPRFWPLWCGLGLLWLIVQLPYPALLAIGRFLGALMYRFAGDRRRIAKRNLELCFPEKSAAERKRLLKE
NFASTGIAFFEMAMSWWWSRQRLAKLAHVEGLEHLQKAQREGKGVILMAVHFTTLEIGAALLGQQHTIDGMYREHKNPLF
DFVQRQGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
QERLADGSGYRLVIHPPLEGFPGETEEEDCIRINQWVEGVLRECPEQYLWAHRRFKSRPPGEPKLYAKRG

Sequences:

>Translated_310_residues
MDRPRFRKAFLAPRFWPLWCGLGLLWLIVQLPYPALLAIGRFLGALMYRFAGDRRRIAKRNLELCFPEKSAAERKRLLKE
NFASTGIAFFEMAMSWWWSRQRLAKLAHVEGLEHLQKAQREGKGVILMAVHFTTLEIGAALLGQQHTIDGMYREHKNPLF
DFVQRQGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
QERLADGSGYRLVIHPPLEGFPGETEEEDCIRINQWVEGVLRECPEQYLWAHRRFKSRPPGEPKLYAKRG
>Mature_310_residues
MDRPRFRKAFLAPRFWPLWCGLGLLWLIVQLPYPALLAIGRFLGALMYRFAGDRRRIAKRNLELCFPEKSAAERKRLLKE
NFASTGIAFFEMAMSWWWSRQRLAKLAHVEGLEHLQKAQREGKGVILMAVHFTTLEIGAALLGQQHTIDGMYREHKNPLF
DFVQRQGRERHNLDSLAVERDDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
QERLADGSGYRLVIHPPLEGFPGETEEEDCIRINQWVEGVLRECPEQYLWAHRRFKSRPPGEPKLYAKRG

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=45.4248366013072, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI87082094, Length=303, Percent_Identity=44.8844884488449, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1788161, Length=313, Percent_Identity=31.9488817891374, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35730; Mature: 35730

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPRFRKAFLAPRFWPLWCGLGLLWLIVQLPYPALLAIGRFLGALMYRFAGDRRRIAKR
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHC
NLELCFPEKSAAERKRLLKENFASTGIAFFEMAMSWWWSRQRLAKLAHVEGLEHLQKAQR
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGKGVILMAVHFTTLEIGAALLGQQHTIDGMYREHKNPLFDFVQRQGRERHNLDSLAVER
CCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCHHCCHHHHHHHH
DDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEECCCC
QERLADGSGYRLVIHPPLEGFPGETEEEDCIRINQWVEGVLRECPEQYLWAHRRFKSRPP
HHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
GEPKLYAKRG
CCCCCCCCCC
>Mature Secondary Structure
MDRPRFRKAFLAPRFWPLWCGLGLLWLIVQLPYPALLAIGRFLGALMYRFAGDRRRIAKR
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHC
NLELCFPEKSAAERKRLLKENFASTGIAFFEMAMSWWWSRQRLAKLAHVEGLEHLQKAQR
CCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGKGVILMAVHFTTLEIGAALLGQQHTIDGMYREHKNPLFDFVQRQGRERHNLDSLAVER
CCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCHHCCHHHHHHHH
DDVRGMLKLLRAGRAIWYAPDQDYGAKQSIFVPLFGIQAATVTATSKFARLGKALVVPFT
HHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEECCCC
QERLADGSGYRLVIHPPLEGFPGETEEEDCIRINQWVEGVLRECPEQYLWAHRRFKSRPP
HHHCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
GEPKLYAKRG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]