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The map label for this gene is lpdG [H]

Identifier: 77457843

GI number: 77457843

Start: 1807240

End: 1808676

Strand: Direct

Name: lpdG [H]

Synonym: Pfl01_1616

Alternate gene names: 77457843

Gene position: 1807240-1808676 (Clockwise)

Preceding gene: 77457842

Following gene: 77457844

Centisome position: 28.07

GC content: 58.73

Gene sequence:

>1437_bases
ATGTCGCAGAAATTTGACGTAGTAGTGATCGGTGCGGGCCCTGGCGGCTACGTGGCAGCTATCAAGGCCGCGCAACTCGG
CCTGAGCACTGCCTGCATCGAGAAGTACACCGATGCTGAAGGCAAGCAAGCCCTGGGCGGCACCTGCCTGAACGTAGGCT
GCATTCCTTCCAAGGCGCTGCTGGACAGCTCCTGGAAATACAAGGAAGCGAAAGAAAGCTTCAACGTCCACGGTATCTCG
ACCGGCGAAGTCAAAATGGACGTCGCTGCGATGGTTGGCCGCAAGGCTGGTATCGTCAAGAACCTGACCGGCGGTGTTGC
CACCCTGTTCAAGGCCAACGGCGTTACTTCGATTCAGGGCCACGGCAAACTGCTGGCCGGCAAGAAAGTCGAAGTCACCA
AGCCGGACGGCTCGGTTGAAGTCATCGAAGCCGAAAACGTCATCCTGGCTCCAGGTTCGCGTCCGATCGACATTCCACCG
GCTCCGGTCGATCAGAAAGTCATCGTCGATTCGACTGGCGCTCTGGAATTCCAATCCGTACCTAAACGTCTGGGCGTGAT
CGGCGCTGGCGTGATCGGTCTGGAACTGGGTTCGGTGTGGTCGCGTTTGGGTGCAGAAGTGACTGTTCTGGAAGCCCTGG
ACACCTTCCTGATGGCAGCGGACACCGCGGTTTCCAAGGAAGCGCTGAAAACCCTGACCAAACAGGGTCTGGACATCAAG
CTGGGCGCCCGTGTAACCGGCTCGAAAGTGAACGGCGACGAAGTCGTTGTGAACTACACCGACGCCAACGGCGAACAGAC
CATCACTTTCGACAAGCTGATCGTAGCCGTTGGTCGCCGTCCGGTGACCACTGATCTGCTGGCTGCCGATAGCGGCGTGA
CCCTGGACGAGCGCGGTTTCGTGCACGTTGACGATCACTGCGCCACCACCGTACCGGGCGTTTACGCCATCGGCGACGTG
GTTCGCGGCATGATGCTGGCTCACAAGGCCTCGGAAGAGGGCATCATGGTTGTCGAGCGCATCAAGGGTCACAAGGCCCA
GATGAACTATGACCTGATCCCTTCGGTTATTTATACTCACCCGGAAATCGCATGGGTCGGTAAAACCGAGCAGGCCTTGA
AAGCTGAAGGCGTTGAAGTTAACGTCGGCACCTTCCCGTTCGCAGCATCCGGCCGTGCCATGGCCGCCAACGATACCGGT
GGTTTCGTCAAGGTCATCGCCGATGCCAAGACTGACCGCGTATTGGGCGTGCACGTGATTGGCCCGAGCGCCGCAGAACT
GGTTCAGCAGGGCGCGATCGGTATGGAATTCGGCACCAGCGCTGAAGATCTGGGCATGATGGTTTTCTCCCATCCGACCC
TGTCTGAAGCCTTGCACGAAGCCGCTTTGGCAGTGAATGGCGGCGCCATCCACATTGCCAACCGCAAGAAGCGTTAA

Upstream 100 bases:

>100_bases
TGATAGAAGCAGTCACGCGCTGCAAGCTTCAAGCTGCGGGAAAACGCAGCCTGGCTTGCAGCGCGCGGCTTGAAGCTTTT
CGCTAAAGAGGATTTTTTGA

Downstream 100 bases:

>100_bases
GACACACAATAAGAAACCACGGCGGTACGGCCCGTCGTGAGCCTTGCGTGCAAGACTCACCGCGGAATGTCCGCTGGACG
CAGCCTTGCGTAGCTGCACC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDAEGKQALGGTCLNVGCIPSKALLDSSWKYKEAKESFNVHGIS
TGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQGHGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPP
APVDQKVIVDSTGALEFQSVPKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIK
LGARVTGSKVNGDEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGFVHVDDHCATTVPGVYAIGDV
VRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTHPEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTG
GFVKVIADAKTDRVLGVHVIGPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR

Sequences:

>Translated_478_residues
MSQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDAEGKQALGGTCLNVGCIPSKALLDSSWKYKEAKESFNVHGIS
TGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQGHGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPP
APVDQKVIVDSTGALEFQSVPKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIK
LGARVTGSKVNGDEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGFVHVDDHCATTVPGVYAIGDV
VRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTHPEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTG
GFVKVIADAKTDRVLGVHVIGPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR
>Mature_477_residues
SQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDAEGKQALGGTCLNVGCIPSKALLDSSWKYKEAKESFNVHGIST
GEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQGHGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPPA
PVDQKVIVDSTGALEFQSVPKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIKL
GARVTGSKVNGDEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGFVHVDDHCATTVPGVYAIGDVV
RGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTHPEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTGG
FVKVIADAKTDRVLGVHVIGPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=48.9316239316239, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=28, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=23.8938053097345, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=23.8938053097345, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=23.8938053097345, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=23.8938053097345, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=23.8938053097345, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.5862068965517, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=446, Percent_Identity=24.8878923766816, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=438, Percent_Identity=23.5159817351598, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=40.3361344537815, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI87081717, Length=463, Percent_Identity=28.9416846652268, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=30.0639658848614, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1789915, Length=469, Percent_Identity=27.9317697228145, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=470, Percent_Identity=50.2127659574468, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=27.906976744186, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=28.4796573875803, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=28.4796573875803, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=498, Percent_Identity=24.4979919678715, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.8879668049792, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=31.055900621118, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=470, Percent_Identity=27.6595744680851, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=51.6059957173448, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24640553, Length=486, Percent_Identity=28.6008230452675, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640549, Length=486, Percent_Identity=28.6008230452675, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=28.1893004115226, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=25.9336099585062, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49799; Mature: 49668

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDAEGKQALGGTCLNVGCIPSKAL
CCCCEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHH
LDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQG
HCCCCCCHHHHHCCCEECEECCCEEEHHHHHHCCCCCHHHHCCCCCEEHEECCCEEEECC
HGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPPAPVDQKVIVDSTGALEFQSV
CCEEEECCEEEEECCCCCEEEEEECCEEECCCCCCCCCCCCCCCCEEEEECCCCEEHHHH
PKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIK
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LGARVTGSKVNGDEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGF
ECCEEECCCCCCCEEEEEEECCCCCEEEEHHHHHHHHCCCCCCHHHEECCCCCEECCCCC
VHVDDHCATTVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHHCCC
PEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI
CCEEEECCCHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR
CCCHHHHHHCCCCCEECCCCHHHCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCC
>Mature Secondary Structure 
SQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDAEGKQALGGTCLNVGCIPSKAL
CCCEEEEEEECCCCCEEEEEHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHH
LDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQG
HCCCCCCHHHHHCCCEECEECCCEEEHHHHHHCCCCCHHHHCCCCCEEHEECCCEEEECC
HGKLLAGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPPAPVDQKVIVDSTGALEFQSV
CCEEEECCEEEEECCCCCEEEEEECCEEECCCCCCCCCCCCCCCCEEEEECCCCEEHHHH
PKRLGVIGAGVIGLELGSVWSRLGAEVTVLEALDTFLMAADTAVSKEALKTLTKQGLDIK
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LGARVTGSKVNGDEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGF
ECCEEECCCCCCCEEEEEEECCCCCEEEEHHHHHHHHCCCCCCHHHEECCCCCEECCCCC
VHVDDHCATTVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTH
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHHCCC
PEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI
CCEEEECCCHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEE
GPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHIANRKKR
CCCHHHHHHCCCCCEECCCCHHHCEEEEECCCCHHHHHHHHHEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902462; 2914869 [H]