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The map label for this gene is sucB [H]

Identifier: 77457842

GI number: 77457842

Start: 1805919

End: 1807142

Strand: Direct

Name: sucB [H]

Synonym: Pfl01_1615

Alternate gene names: 77457842

Gene position: 1805919-1807142 (Clockwise)

Preceding gene: 77457841

Following gene: 77457843

Centisome position: 28.05

GC content: 60.87

Gene sequence:

>1224_bases
ATGGCTATCGAGATCAAAGCCCCCACTTTCCCGGAATCGGTTGCCGATGGCACCGTTGCCACCTGGCACAAACAACCGGG
CGACGCCGTCAAGCGTGACGAACTGATCGTCGACATCGAAACCGACAAAGTCGTACTGGAAGTGCTGGCTACCGCCGACG
GCGTGCTGGGCGCAATCGTCAAGGGCGAGGGCGAAACCGTCCTGTCCGACGAAGTCCTGGGCTCCATCGTTGAAGGCGGC
GCTGCATCCGCTGCTCCGGCTGCCGCTGCTGCTCCTGCCGCTGCCGCCGCTGCACCTGCTGCCGCTGATGGCGAAGACGA
CCCGATCGCTGCTCCTGCTGCGCGCAAGCTGGCTGAAGAGAACGGCATCAACATCGCTTCCGTTGCCGGCACCGGCAAAG
GCGGTCGCGTGACCAAGGAAGACGTGGTTGCAGCCGTTGCTGCCAAGAAAGCCGCTCCGGCTGCTGCGCCTGCCAAGGCT
GCTGCTCCGGCCGCCGCCGCTCCTGTGTTCGCCGCTGGCGACCGCATCGAGAAGCGCGTTCCGATGACCCGCGTTCGTGC
CACCGTTGCCAAGCGTCTGGTTGAAGCTCAATCGAACATGGCGATGCTGACCACTTTCAACGAAGTCGACATGACCGAAG
TCATGGCCCTGCGTTCGAAGTACAAGGATCTGTTCGAGAAGTCCCACAACGGCGTACGCCTGGGCTTCATGTCGTTCTTC
GTGAAGGCTGCCACCGAAGCGCTGAAACGCTTCCCGGCGGTCAACGCTTCGATCGATGGCAACGACATCGTTTACCACGG
TTATGCCGACATCGGCGTCGCTGTTTCCAGCGACCGCGGCCTGGTGGTTCCGGTTCTGCGTAACGCCGAACACATGAGCC
TGGCTGAAATCGAAGGCGGCATCGCCACCTTCGGCAAGAAAGCCCGTGACGGCAAACTGTCGATGGACGAAATGACCGGC
GGTACCTTCACCATCACCAACGGTGGTACCTTCGGTTCGATGATGTCGACCCCGATCGTCAACCCGCCGCAGGCAGCGAT
TCTGGGCATGCACAACATCATCCAGCGTCCGATGGCCATCAACGGTCAGGTCGTGATCCGTCCGATGATGTACCTGGCAC
TGTCCTACGATCACCGTCTGATCGATGGCAAAGAAGCTGTAACCTTCCTGGTGACCATCAAGAACCTGCTGGAAGATCCG
GCTCGTCTGTTGCTGGATATCTGA

Upstream 100 bases:

>100_bases
CGCATCGATGCACGCCGAGCAGCAGGAACAACTGCTGCAAGACGCGTTTACCGTTTAACGCCTTCGCGCACCTGAAACCG
AATTTAAGGAACCACAGATA

Downstream 100 bases:

>100_bases
TAGAAGCAGTCACGCGCTGCAAGCTTCAAGCTGCGGGAAAACGCAGCCTGGCTTGCAGCGCGCGGCTTGAAGCTTTTCGC
TAAAGAGGATTTTTTGAATG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAIEIKAPTFPESVADGTVATWHKQPGDAVKRDELIVDIETDKVVLEVLATADGVLGAIVKGEGETVLSDEVLGSIVEGG
AASAAPAAAAAPAAAAAAPAAADGEDDPIAAPAARKLAEENGINIASVAGTGKGGRVTKEDVVAAVAAKKAAPAAAPAKA
AAPAAAAPVFAAGDRIEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFMSFF
VKAATEALKRFPAVNASIDGNDIVYHGYADIGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSMDEMTG
GTFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPMAINGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDP
ARLLLDI

Sequences:

>Translated_407_residues
MAIEIKAPTFPESVADGTVATWHKQPGDAVKRDELIVDIETDKVVLEVLATADGVLGAIVKGEGETVLSDEVLGSIVEGG
AASAAPAAAAAPAAAAAAPAAADGEDDPIAAPAARKLAEENGINIASVAGTGKGGRVTKEDVVAAVAAKKAAPAAAPAKA
AAPAAAAPVFAAGDRIEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFMSFF
VKAATEALKRFPAVNASIDGNDIVYHGYADIGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSMDEMTG
GTFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPMAINGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDP
ARLLLDI
>Mature_406_residues
AIEIKAPTFPESVADGTVATWHKQPGDAVKRDELIVDIETDKVVLEVLATADGVLGAIVKGEGETVLSDEVLGSIVEGGA
ASAAPAAAAAPAAAAAAPAAADGEDDPIAAPAARKLAEENGINIASVAGTGKGGRVTKEDVVAAVAAKKAAPAAAPAKAA
APAAAAPVFAAGDRIEKRVPMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFMSFFV
KAATEALKRFPAVNASIDGNDIVYHGYADIGVAVSSDRGLVVPVLRNAEHMSLAEIEGGIATFGKKARDGKLSMDEMTGG
TFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPMAINGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPA
RLLLDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=248, Percent_Identity=56.8548387096774, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=28.7735849056604, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=29.5154185022026, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=29.5154185022026, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=29.185520361991, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=57.7017114914425, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1786305, Length=306, Percent_Identity=35.6209150326797, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=43.5523114355231, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=31.1363636363636, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=27.1428571428571, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=30.5194805194805, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=44.6384039900249, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.6548672566372, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=244, Percent_Identity=55.327868852459, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=28.2660332541568, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI20129315, Length=446, Percent_Identity=27.8026905829596, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42278; Mature: 42147

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKAPTFPESVADGTVATWHKQPGDAVKRDELIVDIETDKVVLEVLATADGVLGAIV
CEEEECCCCCCHHHCCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEE
KGEGETVLSDEVLGSIVEGGAASAAPAAAAAPAAAAAAPAAADGEDDPIAAPAARKLAEE
ECCCCEEEHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NGINIASVAGTGKGGRVTKEDVVAAVAAKKAAPAAAPAKAAAPAAAAPVFAAGDRIEKRV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHCCCHHHHCC
PMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFMSFF
CHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
VKAATEALKRFPAVNASIDGNDIVYHGYADIGVAVSSDRGLVVPVLRNAEHMSLAEIEGG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHCCC
IATFGKKARDGKLSMDEMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPMAI
HHHHCCCCCCCCCCHHHCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
NGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPARLLLDI
CCCEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AIEIKAPTFPESVADGTVATWHKQPGDAVKRDELIVDIETDKVVLEVLATADGVLGAIV
EEEECCCCCCHHHCCCCEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEE
KGEGETVLSDEVLGSIVEGGAASAAPAAAAAPAAAAAAPAAADGEDDPIAAPAARKLAEE
ECCCCEEEHHHHHHHHHCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHH
NGINIASVAGTGKGGRVTKEDVVAAVAAKKAAPAAAPAKAAAPAAAAPVFAAGDRIEKRV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHCCCHHHHCC
PMTRVRATVAKRLVEAQSNMAMLTTFNEVDMTEVMALRSKYKDLFEKSHNGVRLGFMSFF
CHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
VKAATEALKRFPAVNASIDGNDIVYHGYADIGVAVSSDRGLVVPVLRNAEHMSLAEIEGG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHCCC
IATFGKKARDGKLSMDEMTGGTFTITNGGTFGSMMSTPIVNPPQAAILGMHNIIQRPMAI
HHHHCCCCCCCCCCHHHCCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
NGQVVIRPMMYLALSYDHRLIDGKEAVTFLVTIKNLLEDPARLLLDI
CCCEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]