Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 77457821

GI number: 77457821

Start: 1780279

End: 1781694

Strand: Reverse

Name: pyk [H]

Synonym: Pfl01_1594

Alternate gene names: 77457821

Gene position: 1781694-1780279 (Counterclockwise)

Preceding gene: 77457822

Following gene: 77457820

Centisome position: 27.67

GC content: 64.05

Gene sequence:

>1416_bases
ATGACGCCTGATAAAAAGGTCAAAATCCTCGCCACCCTCGGGCCTGCCGTCGACGGCATCGAAGACATCCGCGAGCTGGT
CGAGGCCGGGGTCAATATCTTCCGCCTCAACTTCAGCCACGGCGACCACGCCGACCACGCCAAGCGCTATCAGTGGATCC
GCGAAGTCGAGCGCCAGCTCAACTACCCGCTCGGGATCCTGATGGACCTGCAAGGGCCGAAGCTGCGGGTCGGCAAGTTC
GCGGACGGCAAGGTTCAGTTGCATCGTGGTCAGGCCTTCCGCCTCGATCTGGATGCGACACCGGGCGACGAACGCCGGGT
AAACCTGCCGCATCCGGAAATCATCGCGGCGCTGGAAGCCGGGATGGATCTGCTGCTGGATGACGGCAAACTGCGCCTGC
GGGTCGTCACCAAATACTCCGACGCGATCGACACCACCGTGCTCAACGGCGGCGAACTGTCGGACCGCAAAGGCGTGAAC
GTGCCGCAAGCGGTGCTCGACCTGAGCCCGCTGACCACCAAGGATCGTCGTGACCTGAGCTTCGGTCTGGAGCTGGGTGT
GGACTGGGTCGCGCTGTCGTTCGTGCAGCGTCCGCAAGACATCCTCGAGGCCCGCGAACTGATCGGCGACAAGGCGTTCC
TGATGGCGAAAATCGAGAAGCCGTCGGCGGTCGAGCAACTGCGCGAGATCGCCGAACTGAGCGACGCGATCATGGTGGCA
CGCGGTGATCTGGGCGTGGAAGTGCCCGCCGAGAGCGTGCCGCAGATCCAGAAAAACATCATCACCACCTGCCGTCATCT
CGGCAAACCGGTGGTGGTGGCGACGCAGATGCTCGAGTCGATGCGCTTCTCCCCGGCCCCGACCCGCGCCGAAGTCACCG
ACGTCGCCAACGCCGTGGCCGAAGGTGCCGATGCGGTGATGCTGTCGGCGGAAACCGCGTCCGGCGAATACCCGCTGGAA
GCCGTGCAGATGATGAGCAAGATCATCCGCCAGGTGGAGAACGGTCCGGACTACCAGACCCAACTCGACGTCAGCCGCCC
GAAAGCCGAAGCGACCGTGTCCGATGCGATCAGCTGCGCAATCCGCCGGATCAGCAACGTGCTGCCGGTGGCGGTGCTGG
TCAACTACAGCGAGTCCGGCGCATCGAGTCTGCGTGCGGCGCGGGAGCGGCCGAAAGCGCCGATCCTGAACCTGACGCCG
AACCTGCAGACCGCCCGCCGGTTGAGCGTGGCGTGGGGCATTCATTCGGTGGTCAATGATCGCCTGCGTCAGGTGGATGA
GGTGTGCTCGACCGCGCTGGAAATCGCTCAGGCGCAAGGAATGGCCGAGCGTGGCGATACGTTGCTGATCACCGCGGGTG
TGCCGTTCGGGCAGCCGGGGTCGACTAATTCGCTGCGGATCGAAACATTGATTTAA

Upstream 100 bases:

>100_bases
ACGGCTACTTCGCGGCGCTCGATGCGCTGATCGTCACCGAGCCGACTCGCACCAACGTCAACGACTTCCGCGCCATCCTG
ATCCTCGAGAGCTCTAAATC

Downstream 100 bases:

>100_bases
GCAACACCTGTGTGGCGAGGGAGCGTGCTCCCGTCGCCCGGCATGACGGTTTCCACAAGAGACGCCGCATGCACCGGGCA
CGAGCTCCCTCGCCACCGTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTPDKKVKILATLGPAVDGIEDIRELVEAGVNIFRLNFSHGDHADHAKRYQWIREVERQLNYPLGILMDLQGPKLRVGKF
ADGKVQLHRGQAFRLDLDATPGDERRVNLPHPEIIAALEAGMDLLLDDGKLRLRVVTKYSDAIDTTVLNGGELSDRKGVN
VPQAVLDLSPLTTKDRRDLSFGLELGVDWVALSFVQRPQDILEARELIGDKAFLMAKIEKPSAVEQLREIAELSDAIMVA
RGDLGVEVPAESVPQIQKNIITTCRHLGKPVVVATQMLESMRFSPAPTRAEVTDVANAVAEGADAVMLSAETASGEYPLE
AVQMMSKIIRQVENGPDYQTQLDVSRPKAEATVSDAISCAIRRISNVLPVAVLVNYSESGASSLRAARERPKAPILNLTP
NLQTARRLSVAWGIHSVVNDRLRQVDEVCSTALEIAQAQGMAERGDTLLITAGVPFGQPGSTNSLRIETLI

Sequences:

>Translated_471_residues
MTPDKKVKILATLGPAVDGIEDIRELVEAGVNIFRLNFSHGDHADHAKRYQWIREVERQLNYPLGILMDLQGPKLRVGKF
ADGKVQLHRGQAFRLDLDATPGDERRVNLPHPEIIAALEAGMDLLLDDGKLRLRVVTKYSDAIDTTVLNGGELSDRKGVN
VPQAVLDLSPLTTKDRRDLSFGLELGVDWVALSFVQRPQDILEARELIGDKAFLMAKIEKPSAVEQLREIAELSDAIMVA
RGDLGVEVPAESVPQIQKNIITTCRHLGKPVVVATQMLESMRFSPAPTRAEVTDVANAVAEGADAVMLSAETASGEYPLE
AVQMMSKIIRQVENGPDYQTQLDVSRPKAEATVSDAISCAIRRISNVLPVAVLVNYSESGASSLRAARERPKAPILNLTP
NLQTARRLSVAWGIHSVVNDRLRQVDEVCSTALEIAQAQGMAERGDTLLITAGVPFGQPGSTNSLRIETLI
>Mature_470_residues
TPDKKVKILATLGPAVDGIEDIRELVEAGVNIFRLNFSHGDHADHAKRYQWIREVERQLNYPLGILMDLQGPKLRVGKFA
DGKVQLHRGQAFRLDLDATPGDERRVNLPHPEIIAALEAGMDLLLDDGKLRLRVVTKYSDAIDTTVLNGGELSDRKGVNV
PQAVLDLSPLTTKDRRDLSFGLELGVDWVALSFVQRPQDILEARELIGDKAFLMAKIEKPSAVEQLREIAELSDAIMVAR
GDLGVEVPAESVPQIQKNIITTCRHLGKPVVVATQMLESMRFSPAPTRAEVTDVANAVAEGADAVMLSAETASGEYPLEA
VQMMSKIIRQVENGPDYQTQLDVSRPKAEATVSDAISCAIRRISNVLPVAVLVNYSESGASSLRAARERPKAPILNLTPN
LQTARRLSVAWGIHSVVNDRLRQVDEVCSTALEIAQAQGMAERGDTLLITAGVPFGQPGSTNSLRIETLI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=484, Percent_Identity=38.8429752066116, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286420, Length=484, Percent_Identity=38.8429752066116, Blast_Score=315, Evalue=8e-86,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=38.7755102040816, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=37.3737373737374, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=37.3737373737374, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=38.86925795053, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=38.86925795053, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=34.6320346320346, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=34.6320346320346, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=34.1121495327103, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=36.7088607594937, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=37.6569037656904, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.0655737704918, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=36.3636363636364, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=36.3636363636364, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=36.3636363636364, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=36.3636363636364, Blast_Score=282, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=34.0909090909091, Blast_Score=253, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=33.6776859504132, Blast_Score=253, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=36.1570247933884, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=36.1570247933884, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=30.7692307692308, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24648966, Length=404, Percent_Identity=35.3960396039604, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24646914, Length=255, Percent_Identity=38.0392156862745, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51384; Mature: 51252

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDKKVKILATLGPAVDGIEDIRELVEAGVNIFRLNFSHGDHADHAKRYQWIREVERQL
CCCCCCEEEEEECCCHHCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
NYPLGILMDLQGPKLRVGKFADGKVQLHRGQAFRLDLDATPGDERRVNLPHPEIIAALEA
CCCEEEEEECCCCCEEECCCCCCEEEEECCCEEEEEECCCCCCCCEECCCCHHHHHHHHH
GMDLLLDDGKLRLRVVTKYSDAIDTTVLNGGELSDRKGVNVPQAVLDLSPLTTKDRRDLS
CCEEEEECCCEEEEEEEEHHHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHH
FGLELGVDWVALSFVQRPQDILEARELIGDKAFLMAKIEKPSAVEQLREIAELSDAIMVA
HHHHHCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEE
RGDLGVEVPAESVPQIQKNIITTCRHLGKPVVVATQMLESMRFSPAPTRAEVTDVANAVA
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EGADAVMLSAETASGEYPLEAVQMMSKIIRQVENGPDYQTQLDVSRPKAEATVSDAISCA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
IRRISNVLPVAVLVNYSESGASSLRAARERPKAPILNLTPNLQTARRLSVAWGIHSVVND
HHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
RLRQVDEVCSTALEIAQAQGMAERGDTLLITAGVPFGQPGSTNSLRIETLI
HHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
TPDKKVKILATLGPAVDGIEDIRELVEAGVNIFRLNFSHGDHADHAKRYQWIREVERQL
CCCCCEEEEEECCCHHCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
NYPLGILMDLQGPKLRVGKFADGKVQLHRGQAFRLDLDATPGDERRVNLPHPEIIAALEA
CCCEEEEEECCCCCEEECCCCCCEEEEECCCEEEEEECCCCCCCCEECCCCHHHHHHHHH
GMDLLLDDGKLRLRVVTKYSDAIDTTVLNGGELSDRKGVNVPQAVLDLSPLTTKDRRDLS
CCEEEEECCCEEEEEEEEHHHHHHHEEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHH
FGLELGVDWVALSFVQRPQDILEARELIGDKAFLMAKIEKPSAVEQLREIAELSDAIMVA
HHHHHCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEE
RGDLGVEVPAESVPQIQKNIITTCRHLGKPVVVATQMLESMRFSPAPTRAEVTDVANAVA
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EGADAVMLSAETASGEYPLEAVQMMSKIIRQVENGPDYQTQLDVSRPKAEATVSDAISCA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHH
IRRISNVLPVAVLVNYSESGASSLRAARERPKAPILNLTPNLQTARRLSVAWGIHSVVND
HHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
RLRQVDEVCSTALEIAQAQGMAERGDTLLITAGVPFGQPGSTNSLRIETLI
HHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA