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The map label for this gene is sbp [H]

Identifier: 77457818

GI number: 77457818

Start: 1777268

End: 1778266

Strand: Reverse

Name: sbp [H]

Synonym: Pfl01_1591

Alternate gene names: 77457818

Gene position: 1778266-1777268 (Counterclockwise)

Preceding gene: 77457819

Following gene: 77457816

Centisome position: 27.62

GC content: 60.66

Gene sequence:

>999_bases
GTGAAAAAACTCTTTGGCGCCTCACTTCTCGCCGCAGGTCTTGCCTTCGGCAGCGTGGCTCAGGCCGCACCGACCCTGCT
CAACGTGTCCTACGACGTGATGCGCGATTTCTACAAGGACTACAACACTGCGTTCCAGAAACACTGGCAGGCCGAACACA
ACGAAAACATCACGGTGCAGATGTCTTTCGGCGGTTCGAGCAAGCAAGCCCGTTCGGTGATCGACGGCCTGCCGGCTGAC
GTCATCACCATGAACATGGCCACCGACATCAACGCCCTGGCCGACAACGGCCAACTGGTGCCGAAGGACTGGGTCACCCG
TCTGCCGAACAACAGCGCGCCGTTCACCTCGGCCACCGTATTCATCGTGCGCAAAGGCAACCCGAAAGCCCTGAAAGACT
GGCCGGACCTGCTCAAGGACGGCGTGCAGGTTATCGTGCCGAACCCGAAAACCTCGGGCAACGGCCGCTACACCTACCTG
TCGGCGTGGGGCTATGTGCTGAAAAACGGCGGTGACGAGAACAAGGCCAAGGACTTCGTCGGCAAGCTGTTCAAACAGGC
ACCGGTGCTGGATACCGGTGGCCGCGCCGCGACCACCACGTTCATGACCAACCAGATCGGCGACGTGCTGGTGACCTTCG
AAAACGAAGCCGAAATGATCGCCCGTGAATTTGGCCGCGACCAGTTCGAAGTCATCTACCCGAGCGTCTCCGCCGAAGCC
GAGCCGCCGGTGTCGGTGGTCGACAAAGTGGTCGAGAAGAAAGGCTCCCGCGCCGCTGCCGAGGAATACCTGAAGTTCCT
GTGGTCGCCGGAAGGTCAGGAAATCGCGGCCGCCAACTACCTGCGTCCACGTGACCCGGCGGTGCTGGCCAAGTACACCG
ACCGTTTCCCGAAAGTCGACTTCCTGTCGGTCGAGAAGACCTTCGGCGACTGGCGCACCGTGCAGAAGACCCACTTCAAC
GATGGCGGGATCTTCGACCAGATCTACAACGGCCAGTAA

Upstream 100 bases:

>100_bases
TTCTTTTTAATCTTTAAACGACTATGCGTGCCCCGCTATAGTCGCTGGCAAATGGCCTCATCTTTGTAAAAAAACACCCC
TACAGAACAAGGAATGCGCA

Downstream 100 bases:

>100_bases
GGCGTGAGCTGATATGAAGAAGGCGACCTGAAGAGGTCGCCTTTTTTATATGCGTTTACATCGGAGGCTTCACGCCATCC
TTGCCCGCCGAAATCGATTG

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKLFGASLLAAGLAFGSVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITVQMSFGGSSKQARSVIDGLPAD
VITMNMATDINALADNGQLVPKDWVTRLPNNSAPFTSATVFIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYL
SAWGYVLKNGGDENKAKDFVGKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
EPPVSVVDKVVEKKGSRAAAEEYLKFLWSPEGQEIAAANYLRPRDPAVLAKYTDRFPKVDFLSVEKTFGDWRTVQKTHFN
DGGIFDQIYNGQ

Sequences:

>Translated_332_residues
MKKLFGASLLAAGLAFGSVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITVQMSFGGSSKQARSVIDGLPAD
VITMNMATDINALADNGQLVPKDWVTRLPNNSAPFTSATVFIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYL
SAWGYVLKNGGDENKAKDFVGKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
EPPVSVVDKVVEKKGSRAAAEEYLKFLWSPEGQEIAAANYLRPRDPAVLAKYTDRFPKVDFLSVEKTFGDWRTVQKTHFN
DGGIFDQIYNGQ
>Mature_332_residues
MKKLFGASLLAAGLAFGSVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITVQMSFGGSSKQARSVIDGLPAD
VITMNMATDINALADNGQLVPKDWVTRLPNNSAPFTSATVFIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYL
SAWGYVLKNGGDENKAKDFVGKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
EPPVSVVDKVVEKKGSRAAAEEYLKFLWSPEGQEIAAANYLRPRDPAVLAKYTDRFPKVDFLSVEKTFGDWRTVQKTHFN
DGGIFDQIYNGQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=316, Percent_Identity=54.746835443038, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=46.7532467532467, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36670; Mature: 36670

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFGASLLAAGLAFGSVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITVQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MSFGGSSKQARSVIDGLPADVITMNMATDINALADNGQLVPKDWVTRLPNNSAPFTSATV
EECCCCCHHHHHHHHCCCHHHEEEEHHHCHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEE
FIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYLSAWGYVLKNGGDENKAKDFV
EEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHH
GKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
HHHHHHCCCCCCCCCEEEHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCCCC
EPPVSVVDKVVEKKGSRAAAEEYLKFLWSPEGQEIAAANYLRPRDPAVLAKYTDRFPKVD
CCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHCCCCCE
FLSVEKTFGDWRTVQKTHFNDGGIFDQIYNGQ
EEEHHHHHCHHHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure
MKKLFGASLLAAGLAFGSVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITVQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MSFGGSSKQARSVIDGLPADVITMNMATDINALADNGQLVPKDWVTRLPNNSAPFTSATV
EECCCCCHHHHHHHHCCCHHHEEEEHHHCHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEE
FIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYLSAWGYVLKNGGDENKAKDFV
EEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHH
GKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
HHHHHHCCCCCCCCCEEEHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCCCC
EPPVSVVDKVVEKKGSRAAAEEYLKFLWSPEGQEIAAANYLRPRDPAVLAKYTDRFPKVD
CCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHCCCCCE
FLSVEKTFGDWRTVQKTHFNDGGIFDQIYNGQ
EEEHHHHHCHHHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]