Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 77457790

GI number: 77457790

Start: 1753146

End: 1753886

Strand: Direct

Name: fliA [H]

Synonym: Pfl01_1563

Alternate gene names: 77457790

Gene position: 1753146-1753886 (Clockwise)

Preceding gene: 77457789

Following gene: 77457791

Centisome position: 27.23

GC content: 60.59

Gene sequence:

>741_bases
ATGACCGCCAGCGGTATGAATTTCTACAAGAAGTCGGCGCGTGACGCGCAGTACGAGCTGATCGAGCGTTACGCGCCACT
GGTCAAACGCATCGCCTACCACTTGCTGGCGCGGTTGCCGGCCAGTGTGCAGGTCGAAGACTTGATCCAGGCCGGGATGA
TCGGCCTGCTCGAAGTCTCGACCAAATACGACGCCAGCAAGGGCGCCAGTTTTGAAACGTACGCGGGCATCCGGATCCGT
GGTGCGATGCTCGATGAAGTCCGTAAGGGGGACTGGGCACCACGCTCGGTTCACCGCAATACCCGCATGGTCAGCGACGC
GATTCGCTCGATTGAAGCTAAAACCGGCCGTGACGCTAAAGATCACGAAGTTGCGGCCGAACTCCAATTGAGTCTCGACG
ATTACTACGGGATTTTGAACGACACCCTGGGCAGCCGCCTGTTCAGTTTCGACGACCTGTTGCAGGACGGCGAACACGAA
GGGCTGCACGAGGATGGCGCCAGTGCTCATCTGGAGCCGTCGCGCGATCTGGAAGACGAACGTTTCCAGGCCGCGCTGGC
GGACGCGATTGCCAATTTGCCGGAGCGTGAGCGACTGGTGTTGGCGCTGTACTACGACGAAGAGCTGAACCTCAAGGAGA
TCGGTGAAGTCCTTGGCGTCAGTGAATCGCGGGTCAGCCAGTTACACAGCCAGTGCGCGGCCCGCTTGCGGGGGCGTTTG
GGGGAGTGGCGAGCGCGCTGA

Upstream 100 bases:

>100_bases
TCGCACAGAAGGTCGACACCTGGCCGCTGCCCGCCAACCCGCGCGGCCACCTCGAATTTTTCGTCGAGCGCCTCGTGCAG
CAAACGGCAGGACCTGTGCT

Downstream 100 bases:

>100_bases
AGGCAGTGTGGGGACACTGCGAACGAGGCTGGTGCGGTGGTGAACGGCACCGGTCTTGCTCTGTTGTGCTCCAGACAGTC
ATCGAGTGTTTTGCCGGATT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTASGMNFYKKSARDAQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDASKGASFETYAGIRIR
GAMLDEVRKGDWAPRSVHRNTRMVSDAIRSIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGEHE
GLHEDGASAHLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
GEWRAR

Sequences:

>Translated_246_residues
MTASGMNFYKKSARDAQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDASKGASFETYAGIRIR
GAMLDEVRKGDWAPRSVHRNTRMVSDAIRSIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGEHE
GLHEDGASAHLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
GEWRAR
>Mature_245_residues
TASGMNFYKKSARDAQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVSTKYDASKGASFETYAGIRIRG
AMLDEVRKGDWAPRSVHRNTRMVSDAIRSIEAKTGRDAKDHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGEHEG
LHEDGASAHLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRLG
EWRAR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=227, Percent_Identity=51.5418502202643, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=27.1604938271605, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27608; Mature: 27477

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASGMNFYKKSARDAQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVS
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
TKYDASKGASFETYAGIRIRGAMLDEVRKGDWAPRSVHRNTRMVSDAIRSIEAKTGRDAK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
DHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGEHEGLHEDGASAHLEPSRDLEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
RFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GEWRAR
HHCCCC
>Mature Secondary Structure 
TASGMNFYKKSARDAQYELIERYAPLVKRIAYHLLARLPASVQVEDLIQAGMIGLLEVS
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHH
TKYDASKGASFETYAGIRIRGAMLDEVRKGDWAPRSVHRNTRMVSDAIRSIEAKTGRDAK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
DHEVAAELQLSLDDYYGILNDTLGSRLFSFDDLLQDGEHEGLHEDGASAHLEPSRDLEDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
RFQAALADAIANLPERERLVLALYYDEELNLKEIGEVLGVSESRVSQLHSQCAARLRGRL
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GEWRAR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]