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The map label for this gene is tam [H]

Identifier: 77457503

GI number: 77457503

Start: 1449010

End: 1449825

Strand: Reverse

Name: tam [H]

Synonym: Pfl01_1276

Alternate gene names: 77457503

Gene position: 1449825-1449010 (Counterclockwise)

Preceding gene: 77457504

Following gene: 77457491

Centisome position: 22.52

GC content: 61.4

Gene sequence:

>816_bases
ATGATCACCCGACTCAACCCCGCTCCCGTCGACAAAGACTGGGACGCCAAGGCGTACCAGCAGTTTTCCCGTCTCAGGCA
ACAACCGGTCGATGAATTGCTCGGCCGTGTCGACCTGGAAAATCCCCAACGCATTTATGACCTGGGCTGCGGCACCGGGA
TTGCCACAAAGATTCTGGCGGATCGCTGGCCCGAGGCAGAGATCAAGGGCATCGACAGTTCGAAAGACATGCTTCAGGTC
GCCCGCTGCCTGCCGATCAAGGCGCGCTGGCAACACGCCGAACTGCAACACTGGAAACCCCGCAAGCCGGCCGACCTGTT
GTTCGCCGCGGCCGTGCTGCATTTCATCGATGACCATCAAACGCTGTTGCCGGGACTGCTTCGCCACCTCAACCCCGGCG
GGTGCCTCGCCGCGCACATGCCGGACTGGCGTGATGCGCTGTGGTATCGGCTGATGCTCGGCACCCTCAACGATGCCGGC
CCCTGCGGCCGCCCCATCGGCACTGCGCAATTGCGCCAGCGCATGGCCGCGCGGACGTTGTTGTCGCTGGAGGATTACTA
CCGGCTGCTGGCTCCGCGGACCCAGTCGCTGGATATCTGGGAAACCGAGCAGTTGCAGGTCGTGCGCGGAAGCTCACCGA
TTTATGACTGGGTGAAGGTGTCGGCGCTGCGGCCGGTGATGCAGGGACTGACGTCAGAGGAACAGGCACGCTTCATTTAT
CACTACCTGAGGCGGGTGCATGCGCACTATCCGCAGGAGGAAAACGGCCATACGTTGTTTGCGTTCAAGCGGATTTTCAT
TGTTGCCAAGGTATGA

Upstream 100 bases:

>100_bases
CTCCACCCCCACCCGCTTCCGTTATCTGTTGATGTTGGTCCCCCAAGACTCGAGCCAGGTTGTCATCGGAAGACCCTGCT
GATCACCGGAGGACCGCCTC

Downstream 100 bases:

>100_bases
AGTTCAAAGGATCGCAGCCTTCAACAGGCTGCGACCCCTCGAATTCGACGATTACTCGCGGGATTCGGTACCCAGCTCAT
CCCACACCGACTCGGCCAGA

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MITRLNPAPVDKDWDAKAYQQFSRLRQQPVDELLGRVDLENPQRIYDLGCGTGIATKILADRWPEAEIKGIDSSKDMLQV
ARCLPIKARWQHAELQHWKPRKPADLLFAAAVLHFIDDHQTLLPGLLRHLNPGGCLAAHMPDWRDALWYRLMLGTLNDAG
PCGRPIGTAQLRQRMAARTLLSLEDYYRLLAPRTQSLDIWETEQLQVVRGSSPIYDWVKVSALRPVMQGLTSEEQARFIY
HYLRRVHAHYPQEENGHTLFAFKRIFIVAKV

Sequences:

>Translated_271_residues
MITRLNPAPVDKDWDAKAYQQFSRLRQQPVDELLGRVDLENPQRIYDLGCGTGIATKILADRWPEAEIKGIDSSKDMLQV
ARCLPIKARWQHAELQHWKPRKPADLLFAAAVLHFIDDHQTLLPGLLRHLNPGGCLAAHMPDWRDALWYRLMLGTLNDAG
PCGRPIGTAQLRQRMAARTLLSLEDYYRLLAPRTQSLDIWETEQLQVVRGSSPIYDWVKVSALRPVMQGLTSEEQARFIY
HYLRRVHAHYPQEENGHTLFAFKRIFIVAKV
>Mature_271_residues
MITRLNPAPVDKDWDAKAYQQFSRLRQQPVDELLGRVDLENPQRIYDLGCGTGIATKILADRWPEAEIKGIDSSKDMLQV
ARCLPIKARWQHAELQHWKPRKPADLLFAAAVLHFIDDHQTLLPGLLRHLNPGGCLAAHMPDWRDALWYRLMLGTLNDAG
PCGRPIGTAQLRQRMAARTLLSLEDYYRLLAPRTQSLDIWETEQLQVVRGSSPIYDWVKVSALRPVMQGLTSEEQARFIY
HYLRRVHAHYPQEENGHTLFAFKRIFIVAKV

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=259, Percent_Identity=37.4517374517375, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 31190; Mature: 31190

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLNPAPVDKDWDAKAYQQFSRLRQQPVDELLGRVDLENPQRIYDLGCGTGIATKILA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHH
DRWPEAEIKGIDSSKDMLQVARCLPIKARWQHAELQHWKPRKPADLLFAAAVLHFIDDHQ
HCCCCCHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHH
TLLPGLLRHLNPGGCLAAHMPDWRDALWYRLMLGTLNDAGPCGRPIGTAQLRQRMAARTL
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LSLEDYYRLLAPRTQSLDIWETEQLQVVRGSSPIYDWVKVSALRPVMQGLTSEEQARFIY
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
HYLRRVHAHYPQEENGHTLFAFKRIFIVAKV
HHHHHHHHHCCCCCCCCEEHHHHHHHHHCCC
>Mature Secondary Structure
MITRLNPAPVDKDWDAKAYQQFSRLRQQPVDELLGRVDLENPQRIYDLGCGTGIATKILA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHH
DRWPEAEIKGIDSSKDMLQVARCLPIKARWQHAELQHWKPRKPADLLFAAAVLHFIDDHQ
HCCCCCHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCHH
TLLPGLLRHLNPGGCLAAHMPDWRDALWYRLMLGTLNDAGPCGRPIGTAQLRQRMAARTL
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LSLEDYYRLLAPRTQSLDIWETEQLQVVRGSSPIYDWVKVSALRPVMQGLTSEEQARFIY
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
HYLRRVHAHYPQEENGHTLFAFKRIFIVAKV
HHHHHHHHHCCCCCCCCEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA