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The map label for this gene is pmbA [H]

Identifier: 77457074

GI number: 77457074

Start: 998146

End: 999492

Strand: Direct

Name: pmbA [H]

Synonym: Pfl01_0847

Alternate gene names: 77457074

Gene position: 998146-999492 (Clockwise)

Preceding gene: 77457072

Following gene: 77457075

Centisome position: 15.5

GC content: 61.99

Gene sequence:

>1347_bases
ATGAGTGCAGTTGAAAGCGTCGGCCCACAGGCATTGCCGGCACTGCAGGAACAGGTCGAGCAGATCATCGCCGAAGCCAG
GCGTCAGGGTGCCAGTGCCTGCGAAGTAGCGGTGTCGCTGGAGCAGGGCCTGTCGACGTCGGTGCGTCAACGTGAAGTCG
AAACGGTCGAGTTCAACCGCGATCAGGGTTTTGGCATCACGCTGTACGTCGGCCAGCGCAAGGGCTCGGCCAGCACCTCT
GCCACCGGGCCGGATGCGATTCGCGAGACCGTCGCTGCGGCGCTGGCCATTGCCAAACACACCTCCGAGGACGAAGCCTC
GGGGCTGGCCGATGCCGCGCTGATGGCCAGGGATATTCAGGATTTCGACCTGTTCCACCAGTGGGACATCACTCCGGAGC
AGGCCATCGAGAAGGCGTTGCTCTGCGAAGCTGCCGCGTTCGACGCTGATGCGCGCATCAAGAATGCCGATGGCACCACG
TTGAGTACCCATCAGGGTTGCCGCGTGTACGGCAACAGCCACGGTTTTATCGGCGGTTACGCTTCGACCCGACACAGCCT
GAGCTGCGTAATGATCGCCGAGGCCGATGGCCAGATGCAGCGCGACTACTGGTATGACGTGAACCGCCAGGGCAGTTTGC
TGGCGGATCCGGTGAGCATCGGCCAGCGTGCTGCGCAGCGGGCGGCGAGTCGCCTGGGCGCGCGTCCGGTGCCGACCTGC
GAAGTGCCGGTTTTGTTTTCGGCGGAACTGGCGGGCGGGTTGTTCGGCAGCTTTCTGTCGGCGATTTCCGGCGGAAGCCT
GTACCGCAAATCGTCGTTCCTCGAAGGCACCCTGGGGCAGAAACTGTTCCCGGAGTGGCTGACCATCGACGAGCGTCCGC
ACCTGATGCGTGCCATGGGCAGTGCTTCCTACGATGGTGACGGTCTGGCGACCTATGCCAAACCGTTCGTCGAGAAGGGC
GAGTTGGTGTCGTACATTCTTGGCACTTATTCCGGTCGCAAACTCGGCATGCCGAGTACCGCCAACGCGGGCGGCGTGCA
CAACCTGTTCGTCACCCATGGCGATGAAGATCAGGCGGCCTTGCTGCGACGCATGGGGCGTGGGTTGTTGGTCACCGAGT
TGATGGGCCATGGTCTGAACATGGTGACCGGTGATTACTCGCGCGGTGCGGCGGGTTTCTGGGTCGAGAACGGTGAAATC
CAGTTCGCGGTGCAGGAAGTGACCATCGCCGGCAACATGCGCGACATGTTCAAACAAATTGTCGCAGTCGGTAACGATCT
GGAACTGCGCAGCAACATTCGCACTGGTTCGGTGTTGATCGAGAGGATGACTGTCGCGGGCAGCTAA

Upstream 100 bases:

>100_bases
TCCGCAAAGAAACGCCGCCATGATAACCAGTCGGGGGCCGCTTGTCCGGCCCGGTCGCTCGAAGGCCCCAGCCGAAAGCA
GAATTTGAGTGGAGATGAGC

Downstream 100 bases:

>100_bases
GCAGCAGGCAGGTACAAAAAAGGCGCGCCACCCGATTGGGAGGCGCGCCTTTTTTGTGTCTGTAAATTTTTCGAGCTGAT
GAGGGCAGCAAAAAAGCTGC

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTS
ATGPDAIRETVAAALAIAKHTSEDEASGLADAALMARDIQDFDLFHQWDITPEQAIEKALLCEAAAFDADARIKNADGTT
LSTHQGCRVYGNSHGFIGGYASTRHSLSCVMIAEADGQMQRDYWYDVNRQGSLLADPVSIGQRAAQRAASRLGARPVPTC
EVPVLFSAELAGGLFGSFLSAISGGSLYRKSSFLEGTLGQKLFPEWLTIDERPHLMRAMGSASYDGDGLATYAKPFVEKG
ELVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAALLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEI
QFAVQEVTIAGNMRDMFKQIVAVGNDLELRSNIRTGSVLIERMTVAGS

Sequences:

>Translated_448_residues
MSAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTS
ATGPDAIRETVAAALAIAKHTSEDEASGLADAALMARDIQDFDLFHQWDITPEQAIEKALLCEAAAFDADARIKNADGTT
LSTHQGCRVYGNSHGFIGGYASTRHSLSCVMIAEADGQMQRDYWYDVNRQGSLLADPVSIGQRAAQRAASRLGARPVPTC
EVPVLFSAELAGGLFGSFLSAISGGSLYRKSSFLEGTLGQKLFPEWLTIDERPHLMRAMGSASYDGDGLATYAKPFVEKG
ELVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAALLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEI
QFAVQEVTIAGNMRDMFKQIVAVGNDLELRSNIRTGSVLIERMTVAGS
>Mature_447_residues
SAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQREVETVEFNRDQGFGITLYVGQRKGSASTSA
TGPDAIRETVAAALAIAKHTSEDEASGLADAALMARDIQDFDLFHQWDITPEQAIEKALLCEAAAFDADARIKNADGTTL
STHQGCRVYGNSHGFIGGYASTRHSLSCVMIAEADGQMQRDYWYDVNRQGSLLADPVSIGQRAAQRAASRLGARPVPTCE
VPVLFSAELAGGLFGSFLSAISGGSLYRKSSFLEGTLGQKLFPEWLTIDERPHLMRAMGSASYDGDGLATYAKPFVEKGE
LVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAALLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEIQ
FAVQEVTIAGNMRDMFKQIVAVGNDLELRSNIRTGSVLIERMTVAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=425, Percent_Identity=50.5882352941176, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789640, Length=412, Percent_Identity=19.6601941747573, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47954; Mature: 47822

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQREVETVEFNR
CCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHEEEECC
DQGFGITLYVGQRKGSASTSATGPDAIRETVAAALAIAKHTSEDEASGLADAALMARDIQ
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DFDLFHQWDITPEQAIEKALLCEAAAFDADARIKNADGTTLSTHQGCRVYGNSHGFIGGY
HHHHHEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCCEEEECCCCCCCCC
ASTRHSLSCVMIAEADGQMQRDYWYDVNRQGSLLADPVSIGQRAAQRAASRLGARPVPTC
CCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCC
EVPVLFSAELAGGLFGSFLSAISGGSLYRKSSFLEGTLGQKLFPEWLTIDERPHLMRAMG
CCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHC
SASYDGDGLATYAKPFVEKGELVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHH
LLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEIQFAVQEVTIAGNMRDMFKQI
HHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCEEEEEEEEEEECCCHHHHHHHH
VAVGNDLELRSNIRTGSVLIERMTVAGS
HHCCCCCHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAVESVGPQALPALQEQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQREVETVEFNR
CCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHEEEECC
DQGFGITLYVGQRKGSASTSATGPDAIRETVAAALAIAKHTSEDEASGLADAALMARDIQ
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DFDLFHQWDITPEQAIEKALLCEAAAFDADARIKNADGTTLSTHQGCRVYGNSHGFIGGY
HHHHHEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEECCCCCEEEECCCCCCCCC
ASTRHSLSCVMIAEADGQMQRDYWYDVNRQGSLLADPVSIGQRAAQRAASRLGARPVPTC
CCCCCCEEEEEEECCCCCCCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCC
EVPVLFSAELAGGLFGSFLSAISGGSLYRKSSFLEGTLGQKLFPEWLTIDERPHLMRAMG
CCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHC
SASYDGDGLATYAKPFVEKGELVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGDEDQAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHH
LLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEIQFAVQEVTIAGNMRDMFKQI
HHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCEEEEEEEEEEECCCHHHHHHHH
VAVGNDLELRSNIRTGSVLIERMTVAGS
HHCCCCCHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]