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The map label for this gene is yafV [C]

Identifier: 77457071

GI number: 77457071

Start: 995153

End: 996013

Strand: Direct

Name: yafV [C]

Synonym: Pfl01_0844

Alternate gene names: 77457071

Gene position: 995153-996013 (Clockwise)

Preceding gene: 77457070

Following gene: 77457072

Centisome position: 15.46

GC content: 63.76

Gene sequence:

>861_bases
ATGTCTTTAGCGGTGATTCAAATGGTCAGCCAGAGCGATGTGCTGGCCAATCTGCGCGATGCCCGACGTTTGCTCGAACA
CGCATCTAACGCCGGTGCGAAGCTGGCGGTGCTGCCGGAAAATTTCGCGGCCATGGGCCGTCGCGACATCGCCGATATCG
GCCGCGCCGAAGCACTCGGCGAAGGCCCGATTCTGCCGTGGTTGAAACAGACCGCCCGTGACCTCAAGTTATGGATTGTG
GCCGGCACCTTGCCGTTGCCGCCGGTGGGGCAGCCTGAGGCCAAGTCCCATGCCTGCTCGCTGCTGGTGAATGATCAGGG
CGAAATCGTTGCGCGCTATGACAAGTTGCACCTGTTCGATGTCGACGTGGCGGACAATCGCGGCCGTTACCGCGAATCCG
ATGACTATGCTTATGGCAGTGGCGTGGTGGTCGCGGACACCCCGGTCGGGCGACTGGGCCTGACGGTGTGCTACGACCTG
CGTTTCCCGGAGCTGTACAGCGAATTGCGCGCAGCCGGGGCTGAACTGATTACCGCACCGTCGGCCTTCACCGCGGTGAC
CGGTGCGGCGCACTGGGATGTACTGATCCGCGCGCGGGCCATTGAAACCCAGTGCTACGTGCTGGCGGCCGCTCAGGGCG
GGACGCATCCGGGGCCGCGGGAAACCTTTGGGCACGGGGCGATTGTCGACCCGTGGGGCCGCGTGCTGGCACAGCAGGAG
CAGGGCGAAGCCGTACTGCTGGCCGAGCGCGACAGCAATGAACAAGCGTCCATCCGGGCGCGAATGCCGGTGACGGGTCA
TCGGCGGTTTTTTTCGCAGGGCGCACAGCGACCTGCTTCAGAACACGAATTTAAGGCGTAA

Upstream 100 bases:

>100_bases
CCGTGGAAAGAGCCGAAAATCACCTTCGACAAGCCTTTTTGAACAGCCAGCCCCTGAACCGATGGAGTAGCATGGCCGCA
TACCTCTTGCGGAGTGCGCC

Downstream 100 bases:

>100_bases
AACCTATGAGCGAGTTGTTGTCCTCAGTCAGTGATCACCTGTTGGCGCCCGGCGGCGTGACGATCGAGAGTCTGCAAGGC
GTGCTCGGTGATCTGGCGGG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSLAVIQMVSQSDVLANLRDARRLLEHASNAGAKLAVLPENFAAMGRRDIADIGRAEALGEGPILPWLKQTARDLKLWIV
AGTLPLPPVGQPEAKSHACSLLVNDQGEIVARYDKLHLFDVDVADNRGRYRESDDYAYGSGVVVADTPVGRLGLTVCYDL
RFPELYSELRAAGAELITAPSAFTAVTGAAHWDVLIRARAIETQCYVLAAAQGGTHPGPRETFGHGAIVDPWGRVLAQQE
QGEAVLLAERDSNEQASIRARMPVTGHRRFFSQGAQRPASEHEFKA

Sequences:

>Translated_286_residues
MSLAVIQMVSQSDVLANLRDARRLLEHASNAGAKLAVLPENFAAMGRRDIADIGRAEALGEGPILPWLKQTARDLKLWIV
AGTLPLPPVGQPEAKSHACSLLVNDQGEIVARYDKLHLFDVDVADNRGRYRESDDYAYGSGVVVADTPVGRLGLTVCYDL
RFPELYSELRAAGAELITAPSAFTAVTGAAHWDVLIRARAIETQCYVLAAAQGGTHPGPRETFGHGAIVDPWGRVLAQQE
QGEAVLLAERDSNEQASIRARMPVTGHRRFFSQGAQRPASEHEFKA
>Mature_285_residues
SLAVIQMVSQSDVLANLRDARRLLEHASNAGAKLAVLPENFAAMGRRDIADIGRAEALGEGPILPWLKQTARDLKLWIVA
GTLPLPPVGQPEAKSHACSLLVNDQGEIVARYDKLHLFDVDVADNRGRYRESDDYAYGSGVVVADTPVGRLGLTVCYDLR
FPELYSELRAAGAELITAPSAFTAVTGAAHWDVLIRARAIETQCYVLAAAQGGTHPGPRETFGHGAIVDPWGRVLAQQEQ
GEAVLLAERDSNEQASIRARMPVTGHRRFFSQGAQRPASEHEFKA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=271, Percent_Identity=42.8044280442804, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI5031947, Length=271, Percent_Identity=42.8044280442804, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI297632350, Length=271, Percent_Identity=42.8044280442804, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=32.8358208955224, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=38.974358974359, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786412, Length=230, Percent_Identity=30, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556280, Length=270, Percent_Identity=39.2592592592593, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322335, Length=298, Percent_Identity=33.2214765100671, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=33.6917562724014, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17933642, Length=270, Percent_Identity=41.8518518518518, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI21355835, Length=281, Percent_Identity=31.3167259786477, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31012; Mature: 30881

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAVIQMVSQSDVLANLRDARRLLEHASNAGAKLAVLPENFAAMGRRDIADIGRAEALG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHHCC
EGPILPWLKQTARDLKLWIVAGTLPLPPVGQPEAKSHACSLLVNDQGEIVARYDKLHLFD
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECEEEEEE
VDVADNRGRYRESDDYAYGSGVVVADTPVGRLGLTVCYDLRFPELYSELRAAGAELITAP
EEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCHHEEECC
SAFTAVTGAAHWDVLIRARAIETQCYVLAAAQGGTHPGPRETFGHGAIVDPWGRVLAQQE
CHHHHHHCCHHHHEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCEECHHHHHHHHHC
QGEAVLLAERDSNEQASIRARMPVTGHRRFFSQGAQRPASEHEFKA
CCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLAVIQMVSQSDVLANLRDARRLLEHASNAGAKLAVLPENFAAMGRRDIADIGRAEALG
CCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCHHHHHHHHHHHCC
EGPILPWLKQTARDLKLWIVAGTLPLPPVGQPEAKSHACSLLVNDQGEIVARYDKLHLFD
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECEEEEEE
VDVADNRGRYRESDDYAYGSGVVVADTPVGRLGLTVCYDLRFPELYSELRAAGAELITAP
EEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCHHEEECC
SAFTAVTGAAHWDVLIRARAIETQCYVLAAAQGGTHPGPRETFGHGAIVDPWGRVLAQQE
CHHHHHHCCHHHHEEEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCEECHHHHHHHHHC
QGEAVLLAERDSNEQASIRARMPVTGHRRFFSQGAQRPASEHEFKA
CCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]