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The map label for this gene is garD [H]

Identifier: 77457057

GI number: 77457057

Start: 978234

End: 979787

Strand: Direct

Name: garD [H]

Synonym: Pfl01_0830

Alternate gene names: 77457057

Gene position: 978234-979787 (Clockwise)

Preceding gene: 77457056

Following gene: 77457058

Centisome position: 15.19

GC content: 62.16

Gene sequence:

>1554_bases
ATGCAGTTGATTGAACATTCCGACTCGCCGCGCTACATCCGCCTGCACGAGCGGGACAACGTAGTGGTAGTGGTCAACGA
CCAGGGCGTGCCGGCCGGCACCGCATTTGCCGATGGCCTGGTGACGGTGGATTTTGTACCGCAGAGTCACAAGGTCAGCC
TCGTGGATATTCCCGAGGGCGGCACGGTGATTCGCTACGGCCAGATCATCGGCTACGCGTTGCAGCCGATTCCCCGTGGC
AGTTGGGTCAAGGAAGATCAACTGCGCATGCCGACCGCGCCGCCGCTGGACAGCCTGCCGCTGTCCACCGATGTGCCGGC
CGCCCAGGCTCCGCTGGAAGGCTTCACGTTCGAGGGTTATCGCAACGCCGACGGTACGGTCGGCACGCGCAACATCCTCG
GGATCACCACTACCGTGCAATGCGTCACCGGGGTGCTGGATCACGCGGTGAAGCGGATCAAGGATGAACTGTTGCCGAAG
TACCCGCACGTCGATGACGTGGTGGCGCTGACCCACAGTTACGGTTGCGGCGTGGCCATCACGGCCACCGACGCCTACAT
CCCGATCCGCACGGTGCGCAATCTGGCGCGCAACCCGAACCTCGGTGGCGAGGCGCTGGTGATCAGTCTGGGCTGCGAAA
AATTGCAGGCCGGGCAGGTGATGCATGAAGACGATGCTTCGGTGGATCTGAGCGAGCCATGGCTGTATCGCTTGCAGGAT
TCCAGTCACGGTTTCACCGAGATGATCGAGCAGATCATGGAGTTGGCTGAAGTCCGACTGAAGAAACTCGACCAGCGCCG
CCGGGAAACCGTGCCGGCGTCCGAGTTGATCCTGGGCATGCAGTGCGGCGGCAGCGATGCGTTTTCCGGGATCACCGCCA
ACCCGGCGCTTGGTTATGCCTCGGACTTGCTGCTGCGTGCGGGGGCGACGGTGATGTTTTCCGAAGTCACCGAAGTGCGC
GACGCGATTTACCTGCTGACCTCACGGGCCCAGACCAAAACCGTTGCCGAGGAACTGGTGCGCGAGATGGACTGGTACGA
CCGTTACCTGGCCAAGGGTGAAGCGGATCGCAGCGCCAACACCACGCCGGGCAACAAGAAGGGCGGGTTGTCGAACATCG
TCGAGAAGTCGCTGGGCTCGATCGTGAAGTCCGGCAGCAGTGCGATCAGCGGCGTGCTCGGCCCCGGGGAGCGCTTCAAG
CAGAAGGGTTTGATTTTTTGTGCGACGCCGGCGAGTGATTTTGTCTGCGGTACGTTGCAACTGGCGGCGGGGATGAACCT
GCATGTGTTCACGACCGGTCGTGGCACGCCTTACGGGTTGGCGATGGCGCCAGTGGTGAAAGTTTCGACCCGTACGGAGC
TGGCGCAGCGCTGGCCGGATCTGATCGACATCGATGCCGGGCGGATTGCTACCGGGCGTGCGTCCATCGAGGAGTTGGGC
TGGGAGTTGTTCCACTACTACCTGGATGTGGCGAGCGGCAAGCAGCAGACGTGGGCGGAGAAGCACAAGCTGCATAACGA
CATTACGTTGTTCAATCCGGCGCCTATCACCTGA

Upstream 100 bases:

>100_bases
TCGGCCCGATCAAACGTGTGGTACTTAAAGAGCCGCCAACCAATGGCGGTTCCGAAGCCACCGGCAAATTGTCTCAAGCG
CATTCCTGAGGAGCGGCGTC

Downstream 100 bases:

>100_bases
GTCTGTTGATCGTTCCCACGCATAGCGTGGGAACGAACGGGGTGAAAGTGGCTTATCATTAGCCCCCTAACGACGCTCAC
CTCCAAGGCTCCCCGGCATG

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MQLIEHSDSPRYIRLHERDNVVVVVNDQGVPAGTAFADGLVTVDFVPQSHKVSLVDIPEGGTVIRYGQIIGYALQPIPRG
SWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPK
YPHVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEDDASVDLSEPWLYRLQD
SSHGFTEMIEQIMELAEVRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQTKTVAEELVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAISGVLGPGERFK
QKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHYYLDVASGKQQTWAEKHKLHNDITLFNPAPIT

Sequences:

>Translated_517_residues
MQLIEHSDSPRYIRLHERDNVVVVVNDQGVPAGTAFADGLVTVDFVPQSHKVSLVDIPEGGTVIRYGQIIGYALQPIPRG
SWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPK
YPHVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEDDASVDLSEPWLYRLQD
SSHGFTEMIEQIMELAEVRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQTKTVAEELVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAISGVLGPGERFK
QKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHYYLDVASGKQQTWAEKHKLHNDITLFNPAPIT
>Mature_517_residues
MQLIEHSDSPRYIRLHERDNVVVVVNDQGVPAGTAFADGLVTVDFVPQSHKVSLVDIPEGGTVIRYGQIIGYALQPIPRG
SWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGYRNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPK
YPHVDDVVALTHSYGCGVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEDDASVDLSEPWLYRLQD
SSHGFTEMIEQIMELAEVRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYASDLLLRAGATVMFSEVTEVR
DAIYLLTSRAQTKTVAEELVREMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAISGVLGPGERFK
QKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
WELFHYYLDVASGKQQTWAEKHKLHNDITLFNPAPIT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=520, Percent_Identity=60.3846153846154, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=493, Percent_Identity=35.6997971602434, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56155; Mature: 56155

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLIEHSDSPRYIRLHERDNVVVVVNDQGVPAGTAFADGLVTVDFVPQSHKVSLVDIPEG
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCEEEEECCCC
GTVIRYGQIIGYALQPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGY
CCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCC
RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEE
TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEDDASVDLSEPWLYRLQD
EECCCEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHCCCCEECCCCCCCCCCCEEEEEEC
SSHGFTEMIEQIMELAEVRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCCCCCCHH
SDLLLRAGATVMFSEVTEVRDAIYLLTSRAQTKTVAEELVREMDWYDRYLAKGEADRSAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTPGNKKGGLSNIVEKSLGSIVKSGSSAISGVLGPGERFKQKGLIFCATPASDFVCGTLQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHCCEEEEECCCCCHHHHHHH
LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
HHCCCEEEEEECCCCCCCCEECCCEEEEHHHHHHHHHCCCEEECCCCCEECCCHHHHHHH
WELFHYYLDVASGKQQTWAEKHKLHNDITLFNPAPIT
HHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MQLIEHSDSPRYIRLHERDNVVVVVNDQGVPAGTAFADGLVTVDFVPQSHKVSLVDIPEG
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHCCEEEEEEECCCCCEEEEECCCC
GTVIRYGQIIGYALQPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGY
CCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCC
RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEE
TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHEDDASVDLSEPWLYRLQD
EECCCEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHCCCCEECCCCCCCCCCCEEEEEEC
SSHGFTEMIEQIMELAEVRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCCCCCCHH
SDLLLRAGATVMFSEVTEVRDAIYLLTSRAQTKTVAEELVREMDWYDRYLAKGEADRSAN
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTPGNKKGGLSNIVEKSLGSIVKSGSSAISGVLGPGERFKQKGLIFCATPASDFVCGTLQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHCCEEEEECCCCCHHHHHHH
LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEELG
HHCCCEEEEEECCCCCCCCEECCCEEEEHHHHHHHHHCCCEEECCCCCEECCCHHHHHHH
WELFHYYLDVASGKQQTWAEKHKLHNDITLFNPAPIT
HHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]