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The map label for this gene is carA [H]

Identifier: 77456993

GI number: 77456993

Start: 899309

End: 900469

Strand: Direct

Name: carA [H]

Synonym: Pfl01_0766

Alternate gene names: 77456993

Gene position: 899309-900469 (Clockwise)

Preceding gene: 77456992

Following gene: 77456994

Centisome position: 13.97

GC content: 61.07

Gene sequence:

>1161_bases
ATGCGTTATTTACGGGAGGTCTTCTTGACTAAGCCAGCCATACTCGCCCTTGCTGATGGCAGCATTTTTCGCGGCGAAGC
CATTGGAGCCGACGGTCAGACCGTTGGTGAGGTGGTGTTCAACACCGCAATGACCGGCTATCAGGAAATCCTTACCGATC
CTTCCTACGCCCAACAGATCGTTACCCTGACTTACCCGCACATCGGCAACACCGGCACCACGCCGGAAGACGCCGAGTCC
GATCGCGTCTGGTCCGCTGGCCTGGTCATTCGTGACCTGCCGCTGGTAGCGAGCAACTGGCGTAACACGATGTCCCTGTC
CGATTACCTGAAAGCCAACAATGTTGTGGCGATCGCCGGTATCGACACCCGCCGCCTGACCCGCATCCTGCGTGAAAAAG
GCGCACAGAACGGCTGCATCATGGCCGGCGACAACATCTCCGAAGAGGCGGCCATCGCCGCGGCGCAAGGCTTCCCGGGC
CTGAAGGGCATGGATCTGGCGAAAGTCGTCAGCACCAAGACCCAATACGAATGGCGCTCCACTGTCTGGGATCTGAAAAC
CGACAGCCACGCGACCATCGAAGCCTCCGAGCTGCCTTACCACGTGGTTGCCTACGACTACGGCGTCAAGGTCAACATCC
TGCGCATGTTGGTCGAGCGCGGCTGCCGCGTCACTGTCGTTCCGGCACAGACCCCGGCGGCCGACGTGCTGGCCTTGAAG
CCGGACGGCGTGTTCCTGTCCAACGGTCCTGGTGATCCGGAGCCTTGCGACTACGCGATCCAAGCGATCAAGGAAGTGCT
GGAAACCGAAATTCCAGTCTTCGGCATCTGCCTCGGTCACCAGCTGCTGGCTCTGGCCTCCGGCGCCAAGACCCTGAAAA
TGGGCCACGGCCACCACGGTGCCAACCACCCGGTGCAGGATCTGGACACTGGCGTCGTGATGATCACCAGCCAGAACCAC
GGTTTCGCGGTTGACGAAGAAACCCTGCCAGCCAACGTCCGCGCGATCCATAAATCGCTGTTCGACGGCACCCTGCAAGG
CATCGAGCGCACCGACAAGAGCGCGTTCAGCTTCCAGGGTCACCCTGAGGCGAGCCCGGGCCCGAACGATGTGGCCCCTC
TGTTTGACCGCTTCATCAACGAGATGGCCAAGCGACGCTAA

Upstream 100 bases:

>100_bases
TTAGTGTGTCCACTAAAAGCGCGCAGAATAATTCAGTGAAGAAGCGGGGTGACGTGTCCATACGTCACTCCGCTTTTTTA
CAACCTGCGATTGCCCTTTC

Downstream 100 bases:

>100_bases
GCGCTCGCCTTGAGACTGTAGCGAGAAGCCCTTGAGGGCGGCCCCGGAACCGGCGGCCCCCTCGGGACTTCAGAAATCGA
TCAAGACGGCTTGCCGACTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MRYLREVFLTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAES
DRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTRILREKGAQNGCIMAGDNISEEAAIAAAQGFPG
LKGMDLAKVVSTKTQYEWRSTVWDLKTDSHATIEASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPAADVLALK
PDGVFLSNGPGDPEPCDYAIQAIKEVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDLDTGVVMITSQNH
GFAVDEETLPANVRAIHKSLFDGTLQGIERTDKSAFSFQGHPEASPGPNDVAPLFDRFINEMAKRR

Sequences:

>Translated_386_residues
MRYLREVFLTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAES
DRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTRILREKGAQNGCIMAGDNISEEAAIAAAQGFPG
LKGMDLAKVVSTKTQYEWRSTVWDLKTDSHATIEASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPAADVLALK
PDGVFLSNGPGDPEPCDYAIQAIKEVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDLDTGVVMITSQNH
GFAVDEETLPANVRAIHKSLFDGTLQGIERTDKSAFSFQGHPEASPGPNDVAPLFDRFINEMAKRR
>Mature_386_residues
MRYLREVFLTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAES
DRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTRILREKGAQNGCIMAGDNISEEAAIAAAQGFPG
LKGMDLAKVVSTKTQYEWRSTVWDLKTDSHATIEASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPAADVLALK
PDGVFLSNGPGDPEPCDYAIQAIKEVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDLDTGVVMITSQNH
GFAVDEETLPANVRAIHKSLFDGTLQGIERTDKSAFSFQGHPEASPGPNDVAPLFDRFINEMAKRR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=379, Percent_Identity=39.8416886543536, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=36.1757105943152, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=36.1757105943152, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=69.5767195767196, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1789760, Length=145, Percent_Identity=28.2758620689655, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=39.1857506361323, Blast_Score=250, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=38.8746803069054, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=387, Percent_Identity=37.4677002583979, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45555749, Length=395, Percent_Identity=38.2278481012658, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24642586, Length=395, Percent_Identity=38.2278481012658, Blast_Score=228, Evalue=8e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41795; Mature: 41795

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLREVFLTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQI
CHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHEE
VTLTYPHIGNTGTTPEDAESDRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAG
EEEECCCCCCCCCCCCCCCCCCEEECCEEEECCCHHHHCCCCCCCHHHHHCCCCEEEEEC
IDTRRLTRILREKGAQNGCIMAGDNISEEAAIAAAQGFPGLKGMDLAKVVSTKTQYEWRS
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TVWDLKTDSHATIEASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPAADVLALK
HEEECCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEC
PDGVFLSNGPGDPEPCDYAIQAIKEVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHG
CCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEECCCCCC
ANHPVQDLDTGVVMITSQNHGFAVDEETLPANVRAIHKSLFDGTLQGIERTDKSAFSFQG
CCCCHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
HPEASPGPNDVAPLFDRFINEMAKRR
CCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYLREVFLTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQI
CHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHEE
VTLTYPHIGNTGTTPEDAESDRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAG
EEEECCCCCCCCCCCCCCCCCCEEECCEEEECCCHHHHCCCCCCCHHHHHCCCCEEEEEC
IDTRRLTRILREKGAQNGCIMAGDNISEEAAIAAAQGFPGLKGMDLAKVVSTKTQYEWRS
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
TVWDLKTDSHATIEASELPYHVVAYDYGVKVNILRMLVERGCRVTVVPAQTPAADVLALK
HEEECCCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEC
PDGVFLSNGPGDPEPCDYAIQAIKEVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHG
CCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEECCCCCC
ANHPVQDLDTGVVMITSQNHGFAVDEETLPANVRAIHKSLFDGTLQGIERTDKSAFSFQG
CCCCHHHCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
HPEASPGPNDVAPLFDRFINEMAKRR
CCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]