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The map label for this gene is rfbB1 [H]

Identifier: 77456929

GI number: 77456929

Start: 829100

End: 830182

Strand: Direct

Name: rfbB1 [H]

Synonym: Pfl01_0701

Alternate gene names: 77456929

Gene position: 829100-830182 (Clockwise)

Preceding gene: 77456928

Following gene: 77456930

Centisome position: 12.88

GC content: 60.57

Gene sequence:

>1083_bases
ATGAAAATTTTAGTGACAGGGGGCGCGGGCTTCATTGGCTCGGCCGTGGTGCGGTTTCTGATTGAAGAGACCGAATGTGA
AGTGATCAACGTCGACAAACTGACCTACGCCGGCAATCTCGAATCTCTGGCTGAGGTGTCCGATTCACCGCGTTACCGGT
TCTGTCAGGTCGACATCTGCGACAGGCCGGCGCTGGACGAACTGTTCGCACGGCTGCAACCGGATGCGGTGATGCATCTG
GCCGCCGAGTCCCATGTGGACCGTTCGATCGATGGCCCGCAAGCCTTCATCGAAACCAACATCGTCGGCACCTACACGCT
GCTCGAAGCGGCGCGTGGTTACTGGAACCGACTGGACGCGGCGCGTCAGGCGGCGTTCCGTTTCCATCACATTTCCACCG
ATGAAGTGTACGGCGACCTGGAACCCGAAGATCCGGCATTTACCGAGCGCACGCCGTATGCGCCGAGTTCGCCGTACTCG
GCAACCAAGGCCGGTTCCGATCATCTGGTTCGCGCCTGGCATCGCACCTTTGGCCTGCCGGTGGTCATGAGTAACTGCTC
GAACAATTACGGGCCGTATCACTTCCCGGAAAAACTGATTCCTCACGTCATCCTCAATGCGCTGCAAGGCAAGCTGCTGC
CGGTGTATGGCGATGGCGCGCAGATTCGCGACTGGCTGTTCGTCGAAGATCACGCCCGCGCACTGTATGCGGTGGTTACG
CGCGGTGAGGTCGGGCAGACCTACAACATCGGCGGCCACAACGAGAAGACCAACCTGGAAGTGGTGCACACCCTGTGCGA
TCTGCTCGAAGCACGCGGTGCGGAAAAACCGGCGGGCGTCGCGCACTTCCGCGACCTGATCACCTTCGTCAAGGATCGTC
CGGGCCACGACAAACGCTACGCCGTCGATGCCGGAAAAATTCACGCGACGCTGGGCTGGACGCCGCAGGAAACCTTCGAA
AGCGGCATGCAGAAAACCGTCGACTGGTATCTGGATAACCGCGCCTGGTGGGTGCGGGTGATGTCCGGTGCCTACGCGCT
GGAGCGCCTCGGCGAGCAAAGCGACAAAGCCCTTGTGGCCTGA

Upstream 100 bases:

>100_bases
TCTCGAACAAGGTCTGTCGCAATACTGGGTGGCGTTGTTGAACTGACCCGCACACAGCATCGAAGGACGTTCAATCATCA
TTTTGCCAGGGGAGGCGCTG

Downstream 100 bases:

>100_bases
ACGTTCAGTCATTCAAATCTCACTGCTTGAAAGGAAAAAAGCATGGCCAAGTACAAAGGAATTCTGCTGGCGGGCGGCGC
CGGTTCGCGTCTGCACCCGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKILVTGGAGFIGSAVVRFLIEETECEVINVDKLTYAGNLESLAEVSDSPRYRFCQVDICDRPALDELFARLQPDAVMHL
AAESHVDRSIDGPQAFIETNIVGTYTLLEAARGYWNRLDAARQAAFRFHHISTDEVYGDLEPEDPAFTERTPYAPSSPYS
ATKAGSDHLVRAWHRTFGLPVVMSNCSNNYGPYHFPEKLIPHVILNALQGKLLPVYGDGAQIRDWLFVEDHARALYAVVT
RGEVGQTYNIGGHNEKTNLEVVHTLCDLLEARGAEKPAGVAHFRDLITFVKDRPGHDKRYAVDAGKIHATLGWTPQETFE
SGMQKTVDWYLDNRAWWVRVMSGAYALERLGEQSDKALVA

Sequences:

>Translated_360_residues
MKILVTGGAGFIGSAVVRFLIEETECEVINVDKLTYAGNLESLAEVSDSPRYRFCQVDICDRPALDELFARLQPDAVMHL
AAESHVDRSIDGPQAFIETNIVGTYTLLEAARGYWNRLDAARQAAFRFHHISTDEVYGDLEPEDPAFTERTPYAPSSPYS
ATKAGSDHLVRAWHRTFGLPVVMSNCSNNYGPYHFPEKLIPHVILNALQGKLLPVYGDGAQIRDWLFVEDHARALYAVVT
RGEVGQTYNIGGHNEKTNLEVVHTLCDLLEARGAEKPAGVAHFRDLITFVKDRPGHDKRYAVDAGKIHATLGWTPQETFE
SGMQKTVDWYLDNRAWWVRVMSGAYALERLGEQSDKALVA
>Mature_360_residues
MKILVTGGAGFIGSAVVRFLIEETECEVINVDKLTYAGNLESLAEVSDSPRYRFCQVDICDRPALDELFARLQPDAVMHL
AAESHVDRSIDGPQAFIETNIVGTYTLLEAARGYWNRLDAARQAAFRFHHISTDEVYGDLEPEDPAFTERTPYAPSSPYS
ATKAGSDHLVRAWHRTFGLPVVMSNCSNNYGPYHFPEKLIPHVILNALQGKLLPVYGDGAQIRDWLFVEDHARALYAVVT
RGEVGQTYNIGGHNEKTNLEVVHTLCDLLEARGAEKPAGVAHFRDLITFVKDRPGHDKRYAVDAGKIHATLGWTPQETFE
SGMQKTVDWYLDNRAWWVRVMSGAYALERLGEQSDKALVA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=35.2941176470588, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=27.2455089820359, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI56237023, Length=313, Percent_Identity=27.7955271565495, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56118217, Length=313, Percent_Identity=27.7955271565495, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI189083684, Length=313, Percent_Identity=27.7955271565495, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504031, Length=348, Percent_Identity=24.1379310344828, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=72.5714285714286, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=69.4050991501417, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=25.4957507082153, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786974, Length=354, Percent_Identity=25.9887005649718, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=36.1194029850746, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=33.433734939759, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=25.4491017964072, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=369, Percent_Identity=27.6422764227642, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=371, Percent_Identity=27.4932614555256, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=288, Percent_Identity=26.3888888888889, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=27.6712328767123, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=26.2536873156342, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=28.4530386740331, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24158427, Length=337, Percent_Identity=24.3323442136499, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40194; Mature: 40194

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRFLIEETECEVINVDKLTYAGNLESLAEVSDSPRYRFCQVDIC
CEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCEEECCCHHHHHHHCCCCCEEEEEEECC
DRPALDELFARLQPDAVMHLAAESHVDRSIDGPQAFIETNIVGTYTLLEAARGYWNRLDA
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
ARQAAFRFHHISTDEVYGDLEPEDPAFTERTPYAPSSPYSATKAGSDHLVRAWHRTFGLP
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VVMSNCSNNYGPYHFPEKLIPHVILNALQGKLLPVYGDGAQIRDWLFVEDHARALYAVVT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHEEEEEEHHHHEEEHEEE
RGEVGQTYNIGGHNEKTNLEVVHTLCDLLEARGAEKPAGVAHFRDLITFVKDRPGHDKRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
AVDAGKIHATLGWTPQETFESGMQKTVDWYLDNRAWWVRVMSGAYALERLGEQSDKALVA
EECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRFLIEETECEVINVDKLTYAGNLESLAEVSDSPRYRFCQVDIC
CEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCEEECCCHHHHHHHCCCCCEEEEEEECC
DRPALDELFARLQPDAVMHLAAESHVDRSIDGPQAFIETNIVGTYTLLEAARGYWNRLDA
CCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
ARQAAFRFHHISTDEVYGDLEPEDPAFTERTPYAPSSPYSATKAGSDHLVRAWHRTFGLP
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VVMSNCSNNYGPYHFPEKLIPHVILNALQGKLLPVYGDGAQIRDWLFVEDHARALYAVVT
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHEEEEEEHHHHEEEHEEE
RGEVGQTYNIGGHNEKTNLEVVHTLCDLLEARGAEKPAGVAHFRDLITFVKDRPGHDKRY
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
AVDAGKIHATLGWTPQETFESGMQKTVDWYLDNRAWWVRVMSGAYALERLGEQSDKALVA
EECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]