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The map label for this gene is chaA [H]

Identifier: 77456902

GI number: 77456902

Start: 788677

End: 789768

Strand: Direct

Name: chaA [H]

Synonym: Pfl01_0674

Alternate gene names: 77456902

Gene position: 788677-789768 (Clockwise)

Preceding gene: 77456898

Following gene: 77456906

Centisome position: 12.25

GC content: 59.8

Gene sequence:

>1092_bases
ATGTTCACCATCCTCAAGCAAGAAAGCTTTCTGCTGTTGGCCGTTATCGCCACCTGTGTTGCGTATCCACTGGAGCACTG
GTTGCTGCACAGCGGCCAGATCGTTGCGCTGACGGCCGGGCTGGTGCTGATCGCCTTTATCGTCGCCGCCTCGATGCGCG
TCGCCCATCAGGCCGAACTGCTCGCGGAAAAAGTCGGCGACCCGTACGGCACGATGATCCTGACCCTCGCCGCCGTGCTG
GTGGAAGTGGTGATCCTGGCGATCATGATGAGCAACGAAGCCTCGCCGACGCTGGTGCGCGACACGATTTATTCGGCGGT
GATGCTCGACATCAACGGCATTCTCGGACTCGCCGCGCTGATGGGCGGGATCAAGCATGGCGAGCAGTCCTACAACGATG
ATTCGGCCCGCAGTTACAGCGTGATGATCCTCACCGCCATGGGTGTGTCGATGGTGGTGCCGGAGTTCATTCCCGAGGCC
AACTGGAAAATCTATTCGGCGTTCACCATCGGCGCGATGGTGGTGCTTTATGCGTTGTTCCTGCGCATGCAGGTCGGGCC
GCACAGTTACTTTTTCAGCTACAGCTACCCGGACAAACGCCGCAGGAAAGAGCCCGTGGAACAGGAGCCGAAACCGGTCA
GTCTGGCGCTGTCGATCGGCATTCTGGTGTTCGGTGTGGTGGTGATCGGTGCACTGGCCGAGGTGATGTCCAAGACCCTC
GATCTGGGCCTGGAAGGGACGGGCGCACCGCCGGTGATCACGGCGATTCTGGTGGCGGCGATCTCGGCAGCGCCAGAGAT
ATTGACCGCGTTGCGTGCAGCGCTGGCCAATCGCATGCAGTCGGTGGTCAACATTGCGATGGGCGCTTCGTTATCGACGG
TGATTCTGACGGTGCCGGTGATGGAGGCGATGGCGCTCTACACGGGGCAGCCGTTTCAGATGGCGATGACGCCGGTGCAG
ACGGTGATGATCTTCATCACGCTGATCGTCAGCGCGATCAACCTCAATGACGGCGAGACCAATGCGATTGAGGGCATGAC
CCACTTTGTGTTGTTTGCGACGTTTATCATGTTGTCGCTGCTGGGGTTGTGA

Upstream 100 bases:

>100_bases
AGATAAAGCTGCCATCCACTGGTGCGCTAAGGTGTAACGAAACGTTAGCGCCACCCCGCAGTCAGTAGCTCATTCGCACT
CTTCGGCAAAAAGGCCCGTC

Downstream 100 bases:

>100_bases
TTGATCGTTCCCACGCGGACGGTTCGACGCCTCGACGTGGGAACGATCAGGTCGGGAGGGGGAATCAGGTACCGGCAATC
AACTGCCGAGCCGCCTGGCT

Product: Sodium/calcium exchanger membrane region

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MFTILKQESFLLLAVIATCVAYPLEHWLLHSGQIVALTAGLVLIAFIVAASMRVAHQAELLAEKVGDPYGTMILTLAAVL
VEVVILAIMMSNEASPTLVRDTIYSAVMLDINGILGLAALMGGIKHGEQSYNDDSARSYSVMILTAMGVSMVVPEFIPEA
NWKIYSAFTIGAMVVLYALFLRMQVGPHSYFFSYSYPDKRRRKEPVEQEPKPVSLALSIGILVFGVVVIGALAEVMSKTL
DLGLEGTGAPPVITAILVAAISAAPEILTALRAALANRMQSVVNIAMGASLSTVILTVPVMEAMALYTGQPFQMAMTPVQ
TVMIFITLIVSAINLNDGETNAIEGMTHFVLFATFIMLSLLGL

Sequences:

>Translated_363_residues
MFTILKQESFLLLAVIATCVAYPLEHWLLHSGQIVALTAGLVLIAFIVAASMRVAHQAELLAEKVGDPYGTMILTLAAVL
VEVVILAIMMSNEASPTLVRDTIYSAVMLDINGILGLAALMGGIKHGEQSYNDDSARSYSVMILTAMGVSMVVPEFIPEA
NWKIYSAFTIGAMVVLYALFLRMQVGPHSYFFSYSYPDKRRRKEPVEQEPKPVSLALSIGILVFGVVVIGALAEVMSKTL
DLGLEGTGAPPVITAILVAAISAAPEILTALRAALANRMQSVVNIAMGASLSTVILTVPVMEAMALYTGQPFQMAMTPVQ
TVMIFITLIVSAINLNDGETNAIEGMTHFVLFATFIMLSLLGL
>Mature_363_residues
MFTILKQESFLLLAVIATCVAYPLEHWLLHSGQIVALTAGLVLIAFIVAASMRVAHQAELLAEKVGDPYGTMILTLAAVL
VEVVILAIMMSNEASPTLVRDTIYSAVMLDINGILGLAALMGGIKHGEQSYNDDSARSYSVMILTAMGVSMVVPEFIPEA
NWKIYSAFTIGAMVVLYALFLRMQVGPHSYFFSYSYPDKRRRKEPVEQEPKPVSLALSIGILVFGVVVIGALAEVMSKTL
DLGLEGTGAPPVITAILVAAISAAPEILTALRAALANRMQSVVNIAMGASLSTVILTVPVMEAMALYTGQPFQMAMTPVQ
TVMIFITLIVSAINLNDGETNAIEGMTHFVLFATFIMLSLLGL

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=356, Percent_Identity=30.6179775280899, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320075, Length=308, Percent_Identity=23.0519480519481, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 38902; Mature: 38902

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTILKQESFLLLAVIATCVAYPLEHWLLHSGQIVALTAGLVLIAFIVAASMRVAHQAEL
CCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEKVGDPYGTMILTLAAVLVEVVILAIMMSNEASPTLVRDTIYSAVMLDINGILGLAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGGIKHGEQSYNDDSARSYSVMILTAMGVSMVVPEFIPEANWKIYSAFTIGAMVVLYALF
HHHHHCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
LRMQVGPHSYFFSYSYPDKRRRKEPVEQEPKPVSLALSIGILVFGVVVIGALAEVMSKTL
HHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGLEGTGAPPVITAILVAAISAAPEILTALRAALANRMQSVVNIAMGASLSTVILTVPV
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MEAMALYTGQPFQMAMTPVQTVMIFITLIVSAINLNDGETNAIEGMTHFVLFATFIMLSL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGL
HCC
>Mature Secondary Structure
MFTILKQESFLLLAVIATCVAYPLEHWLLHSGQIVALTAGLVLIAFIVAASMRVAHQAEL
CCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEKVGDPYGTMILTLAAVLVEVVILAIMMSNEASPTLVRDTIYSAVMLDINGILGLAAL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGGIKHGEQSYNDDSARSYSVMILTAMGVSMVVPEFIPEANWKIYSAFTIGAMVVLYALF
HHHHHCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
LRMQVGPHSYFFSYSYPDKRRRKEPVEQEPKPVSLALSIGILVFGVVVIGALAEVMSKTL
HHHHCCCCCEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGLEGTGAPPVITAILVAAISAAPEILTALRAALANRMQSVVNIAMGASLSTVILTVPV
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MEAMALYTGQPFQMAMTPVQTVMIFITLIVSAINLNDGETNAIEGMTHFVLFATFIMLSL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]