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The map label for this gene is dmlR [H]

Identifier: 77456896

GI number: 77456896

Start: 781733

End: 782662

Strand: Direct

Name: dmlR [H]

Synonym: Pfl01_0668

Alternate gene names: 77456896

Gene position: 781733-782662 (Clockwise)

Preceding gene: 77456892

Following gene: 77456897

Centisome position: 12.14

GC content: 63.55

Gene sequence:

>930_bases
ATGCTGCGTTTCGATGACTTGCAGTTGTTTGTCCGGGCGGCGGATCTGGGCAGCCTGTCGGCGGCGGCTCGGGTGATGGA
CCTGTCCGCCGCTGTGGCCAGTGCCGCGCTCAAACGTATAGAACAGCAACTGGGCGCGCGGCTGTTGGCACGCTCCACTC
GCAGTTTGCGGCTGACGGCCGAAGGCGAGGGGTTTCTCGAGTACGCCCGGGCGGCGCTGAGCAACCTCGATGAGGGTCGG
CGTCTGCTGGCCAGCGGGCAGGATCAGGTCAGCGGGATTCTGCAGCTGTCGGCGCCTTCGGATTTCGGTCGCAACCTGCT
GTTGCCGTGGCTCGACGATTTTCAGCACGAACACCCGAAGCTGACGGTACGTCTGCTGCTGGGTGACCGCATCGCTGATC
TGTTTCGCCAGCCGGTAGACATCGCCTTGCGCTACGGCGAGCCGGAGGATTCGAGCCTGGTGGCGTTGCCGATTGCCCCG
CACAACCGACGGGTACTGTGCGCATCCCCCGCCTACCTGGCCAGGCACGGCGAGCCGCTGCATCTGGAACAACTCGCGCA
GCACAACTGCCTGCTGTACATGCTCGGCGGCCGGGTACATGACCATTGGAGTTTTCATGACGGCAGGCGCGAGGTCGGCC
TGACCGTCAGTGGCGACCGCTTCAGTGACGACGCCGATGTGGTTCGCCTGTGGGCGGTGGCGGGAGCCGGCATCGCTTAC
AAGTCCTGGCTCGATGTGGCCGGTGATGTGCTGGCGGGACGGCTGAAAGTGCTGTTGCCGGAGTTGCTCTGCGAACGCGC
TCCGCTGAATCTGCTGTGCGCCCATCGCGCACAATTGAGTAAGCCGGTGAACCTTCTGAGGGAAATGCTCGCCAGTCGTT
GCGCCGAATTGAGTAGCCGATTTCCCGTTTTCACGGAGACTGATCATTAG

Upstream 100 bases:

>100_bases
GATGAATTCGATGGATGGCATGATTGGCTATTTCTGCCGGAGATAAAACCGGCTAAAAGAGCAGTCTCTTTCAATATTTT
TTTGATAATCGGGGCTGGGA

Downstream 100 bases:

>100_bases
TCGCAGGTAAATAGCGAAATTTCTCTCAGGAACAATCACCAGCCACGGGCGCTCCGGCAGAGGACGCAGCGAGCAACCCG
CTTATACTAGCGGCCGCCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLRFDDLQLFVRAADLGSLSAAARVMDLSAAVASAALKRIEQQLGARLLARSTRSLRLTAEGEGFLEYARAALSNLDEGR
RLLASGQDQVSGILQLSAPSDFGRNLLLPWLDDFQHEHPKLTVRLLLGDRIADLFRQPVDIALRYGEPEDSSLVALPIAP
HNRRVLCASPAYLARHGEPLHLEQLAQHNCLLYMLGGRVHDHWSFHDGRREVGLTVSGDRFSDDADVVRLWAVAGAGIAY
KSWLDVAGDVLAGRLKVLLPELLCERAPLNLLCAHRAQLSKPVNLLREMLASRCAELSSRFPVFTETDH

Sequences:

>Translated_309_residues
MLRFDDLQLFVRAADLGSLSAAARVMDLSAAVASAALKRIEQQLGARLLARSTRSLRLTAEGEGFLEYARAALSNLDEGR
RLLASGQDQVSGILQLSAPSDFGRNLLLPWLDDFQHEHPKLTVRLLLGDRIADLFRQPVDIALRYGEPEDSSLVALPIAP
HNRRVLCASPAYLARHGEPLHLEQLAQHNCLLYMLGGRVHDHWSFHDGRREVGLTVSGDRFSDDADVVRLWAVAGAGIAY
KSWLDVAGDVLAGRLKVLLPELLCERAPLNLLCAHRAQLSKPVNLLREMLASRCAELSSRFPVFTETDH
>Mature_309_residues
MLRFDDLQLFVRAADLGSLSAAARVMDLSAAVASAALKRIEQQLGARLLARSTRSLRLTAEGEGFLEYARAALSNLDEGR
RLLASGQDQVSGILQLSAPSDFGRNLLLPWLDDFQHEHPKLTVRLLLGDRIADLFRQPVDIALRYGEPEDSSLVALPIAP
HNRRVLCASPAYLARHGEPLHLEQLAQHNCLLYMLGGRVHDHWSFHDGRREVGLTVSGDRFSDDADVVRLWAVAGAGIAY
KSWLDVAGDVLAGRLKVLLPELLCERAPLNLLCAHRAQLSKPVNLLREMLASRCAELSSRFPVFTETDH

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=304, Percent_Identity=30.2631578947368, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=28.3276450511945, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=31.25, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787128, Length=282, Percent_Identity=28.3687943262411, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786401, Length=258, Percent_Identity=29.4573643410853, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789639, Length=308, Percent_Identity=27.9220779220779, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=26.4604810996564, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=29.4820717131474, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=29.3729372937294, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=27.4285714285714, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=175, Percent_Identity=26.2857142857143, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=24.3093922651934, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34145; Mature: 34145

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFDDLQLFVRAADLGSLSAAARVMDLSAAVASAALKRIEQQLGARLLARSTRSLRLTA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
EGEGFLEYARAALSNLDEGRRLLASGQDQVSGILQLSAPSDFGRNLLLPWLDDFQHEHPK
CCCHHHHHHHHHHHCHHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCHHHHCCCCCC
LTVRLLLGDRIADLFRQPVDIALRYGEPEDSSLVALPIAPHNRRVLCASPAYLARHGEPL
EEEEEEHHHHHHHHHHCCHHEEEECCCCCCCCEEEEEECCCCCEEEEECCHHHHHCCCCC
HLEQLAQHNCLLYMLGGRVHDHWSFHDGRREVGLTVSGDRFSDDADVVRLWAVAGAGIAY
CHHHHHHCCEEEEEECCEEECCCCCCCCCEEECEEEECCCCCCCHHHHHHHHHHCCCHHH
KSWLDVAGDVLAGRLKVLLPELLCERAPLNLLCAHRAQLSKPVNLLREMLASRCAELSSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPVFTETDH
CCCEECCCC
>Mature Secondary Structure
MLRFDDLQLFVRAADLGSLSAAARVMDLSAAVASAALKRIEQQLGARLLARSTRSLRLTA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
EGEGFLEYARAALSNLDEGRRLLASGQDQVSGILQLSAPSDFGRNLLLPWLDDFQHEHPK
CCCHHHHHHHHHHHCHHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCHHHHCCCCCC
LTVRLLLGDRIADLFRQPVDIALRYGEPEDSSLVALPIAPHNRRVLCASPAYLARHGEPL
EEEEEEHHHHHHHHHHCCHHEEEECCCCCCCCEEEEEECCCCCEEEEECCHHHHHCCCCC
HLEQLAQHNCLLYMLGGRVHDHWSFHDGRREVGLTVSGDRFSDDADVVRLWAVAGAGIAY
CHHHHHHCCEEEEEECCEEECCCCCCCCCEEECEEEECCCCCCCHHHHHHHHHHCCCHHH
KSWLDVAGDVLAGRLKVLLPELLCERAPLNLLCAHRAQLSKPVNLLREMLASRCAELSSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPVFTETDH
CCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]