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The map label for this gene is ydgK [H]

Identifier: 77456892

GI number: 77456892

Start: 778723

End: 779928

Strand: Direct

Name: ydgK [H]

Synonym: Pfl01_0664

Alternate gene names: 77456892

Gene position: 778723-779928 (Clockwise)

Preceding gene: 77456891

Following gene: 77456896

Centisome position: 12.09

GC content: 65.34

Gene sequence:

>1206_bases
ATGAACTTCCGTACCCTTCTGATTCTCGGTGCCCTGACCGCTTTTGGTCCGTTGGCGATCGACTTCTATCTACCCGCCTT
TCCCACCATGGCGCTGGCGTTCGGCACCGATGAGAAACACGTCCAGCTGACGCTGTCGGCTTACTTCTTTGGTCTGTCTA
TTGGTCAACTGGCTTACGGGCCGGTGGCGGATCGCTTCGGCCGGCGGATTCCGCTGCTCATTGGCCTGACGCTGTTCACG
CTGGCGTCAGTGGCCTGCGCCTACGCGCCGAATCTGGAATGGTTGATCGGCGCGCGGCTCGTGCAGGCGCTGGGCGGCTG
TGCGGGGATGGTGATTGCCCGGGCGGTGGTCAGCGACAAGTGCGATGCGGTGGGTTCGGCAAAAGTCTTCTCGCAACTGA
TGCTGGTGATGGGGCTGGCACCGATTCTGGCGCCGATGCTCGGCGGTCTGCTGGTCAACACCACGGGCTGGCAGTCGATC
TTCCTGGTGCTGACAGCGTTCAGTGCCCTCGCCGGGCTGGCGGTGGCCCTCGGCCTGCCGGAAAGTCTGCCGGCGCATGT
TCCGCGTCAGCCGTTGTCCGGTGCCCTGCGTCAGTACGGCCGGCTGCTGGCCGATCCGGTCTACATGGGGCATGCCCTGA
CCGGCGGCATCGCCATTGCCGGGATGTTTGCCTACATCGCCGGCTCCCCGTTCATTTTCATCAAGCTGTATGGCGTACCG
GCCGAGCATTTCGGCTGGCTGTTCGGCACCAACGCGGCCGGGTTCATTCTGGTCGCACAGGTCAATGCGCGCCTGCTGGC
CAAGCGCGGTCCGGCCTTTCTGCTGTCACGGGCGGTCTGGGTTTACCTGGCCGCCGGGCTGACGCTGCTCGCGGTCAGCG
CCTTGCACACCGAGGCCCTGTGGCCACTGCTGATTCCGCTGTTCATCTGCGTGGCCAGCCTTGGCTGCATCAGCCCCAAT
GCGGCGGCGTGTGCGATGAACGGGCAGGGCGGTCGCGCCGGCAGTGCTTCAGCGTTGCTCGGCAGCATGCAATTCAGCGT
CGCCGCCGGGGCTTCGGCGCTGGTGGGCATCCTGCACGATGGCAGCGCCGTGCCGATGGCCATGGTCATCAGCCTGTGCG
GTCTGTTGGTCGTCTGCGCGGCGACACTCACCCGGCGCATGCAGAATGCCCGGGCGCTGGCAAAGGCGCAGGCCGAAATC
CAGTAA

Upstream 100 bases:

>100_bases
AGCGAGCTACCGGAATGTTCAAGGCGTCCTCGCGCGGCTAGACTGTCGGGCTGCACTCCATCGAAGTGCCCCCCAACTGC
CTGCCAGACCTGGACGCCTG

Downstream 100 bases:

>100_bases
CGAAAGCCTCAGCCGACAGCGCGTTGCTGGCCGAGGTCGGGAATCTGATGGGGCGCGCAAATACGCGCTTCGAGGGTGCG
AGTGAAGGCGCGGGCCTCGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNFRTLLILGALTAFGPLAIDFYLPAFPTMALAFGTDEKHVQLTLSAYFFGLSIGQLAYGPVADRFGRRIPLLIGLTLFT
LASVACAYAPNLEWLIGARLVQALGGCAGMVIARAVVSDKCDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSI
FLVLTAFSALAGLAVALGLPESLPAHVPRQPLSGALRQYGRLLADPVYMGHALTGGIAIAGMFAYIAGSPFIFIKLYGVP
AEHFGWLFGTNAAGFILVAQVNARLLAKRGPAFLLSRAVWVYLAAGLTLLAVSALHTEALWPLLIPLFICVASLGCISPN
AAACAMNGQGGRAGSASALLGSMQFSVAAGASALVGILHDGSAVPMAMVISLCGLLVVCAATLTRRMQNARALAKAQAEI
Q

Sequences:

>Translated_401_residues
MNFRTLLILGALTAFGPLAIDFYLPAFPTMALAFGTDEKHVQLTLSAYFFGLSIGQLAYGPVADRFGRRIPLLIGLTLFT
LASVACAYAPNLEWLIGARLVQALGGCAGMVIARAVVSDKCDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSI
FLVLTAFSALAGLAVALGLPESLPAHVPRQPLSGALRQYGRLLADPVYMGHALTGGIAIAGMFAYIAGSPFIFIKLYGVP
AEHFGWLFGTNAAGFILVAQVNARLLAKRGPAFLLSRAVWVYLAAGLTLLAVSALHTEALWPLLIPLFICVASLGCISPN
AAACAMNGQGGRAGSASALLGSMQFSVAAGASALVGILHDGSAVPMAMVISLCGLLVVCAATLTRRMQNARALAKAQAEI
Q
>Mature_401_residues
MNFRTLLILGALTAFGPLAIDFYLPAFPTMALAFGTDEKHVQLTLSAYFFGLSIGQLAYGPVADRFGRRIPLLIGLTLFT
LASVACAYAPNLEWLIGARLVQALGGCAGMVIARAVVSDKCDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSI
FLVLTAFSALAGLAVALGLPESLPAHVPRQPLSGALRQYGRLLADPVYMGHALTGGIAIAGMFAYIAGSPFIFIKLYGVP
AEHFGWLFGTNAAGFILVAQVNARLLAKRGPAFLLSRAVWVYLAAGLTLLAVSALHTEALWPLLIPLFICVASLGCISPN
AAACAMNGQGGRAGSASALLGSMQFSVAAGASALVGILHDGSAVPMAMVISLCGLLVVCAATLTRRMQNARALAKAQAEI
Q

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=374, Percent_Identity=31.5508021390374, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI48994889, Length=410, Percent_Identity=30.7317073170732, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790146, Length=299, Percent_Identity=28.4280936454849, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=28.8629737609329, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790794, Length=386, Percent_Identity=22.7979274611399, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787065, Length=248, Percent_Identity=24.5967741935484, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321577, Length=196, Percent_Identity=22.9591836734694, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=201, Percent_Identity=23.8805970149254, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41573; Mature: 41573

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRTLLILGALTAFGPLAIDFYLPAFPTMALAFGTDEKHVQLTLSAYFFGLSIGQLAYG
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHCC
PVADRFGRRIPLLIGLTLFTLASVACAYAPNLEWLIGARLVQALGGCAGMVIARAVVSDK
CHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSIFLVLTAFSALAGLAVALGLP
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
ESLPAHVPRQPLSGALRQYGRLLADPVYMGHALTGGIAIAGMFAYIAGSPFIFIKLYGVP
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AEHFGWLFGTNAAGFILVAQVNARLLAKRGPAFLLSRAVWVYLAAGLTLLAVSALHTEAL
HHHHCCEEECCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLIPLFICVASLGCISPNAAACAMNGQGGRAGSASALLGSMQFSVAAGASALVGILHD
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
GSAVPMAMVISLCGLLVVCAATLTRRMQNARALAKAQAEIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFRTLLILGALTAFGPLAIDFYLPAFPTMALAFGTDEKHVQLTLSAYFFGLSIGQLAYG
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHCC
PVADRFGRRIPLLIGLTLFTLASVACAYAPNLEWLIGARLVQALGGCAGMVIARAVVSDK
CHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSIFLVLTAFSALAGLAVALGLP
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
ESLPAHVPRQPLSGALRQYGRLLADPVYMGHALTGGIAIAGMFAYIAGSPFIFIKLYGVP
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AEHFGWLFGTNAAGFILVAQVNARLLAKRGPAFLLSRAVWVYLAAGLTLLAVSALHTEAL
HHHHCCEEECCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLIPLFICVASLGCISPNAAACAMNGQGGRAGSASALLGSMQFSVAAGASALVGILHD
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
GSAVPMAMVISLCGLLVVCAATLTRRMQNARALAKAQAEIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]