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The map label for this gene is braF [H]

Identifier: 77456783

GI number: 77456783

Start: 649323

End: 650195

Strand: Direct

Name: braF [H]

Synonym: Pfl01_0555

Alternate gene names: 77456783

Gene position: 649323-650195 (Clockwise)

Preceding gene: 77456782

Following gene: 77456784

Centisome position: 10.09

GC content: 60.14

Gene sequence:

>873_bases
ATGAGTGAGGTCGTACTCTCTGTTGAAAAACTGATGATGCACTTCGGTGGCATCAAGGCCTTGAGCGACGTCAGCCTGAA
GGTCAAACGCAACTCGATCTTCGCCCTGATCGGCCCCAACGGCGCCGGCAAGACCACGGTGTTCAACTGCCTGACCGGGT
TCTACAAGGCTTCCGGCGGCAAGATCGAACTCAACGTGCGCGGCCAGCAGACCAACGTCATCCAGCTGCTGGGCGAGTCG
TTCAAACCGACCGACTTCGTCTCGCCGAAAAACTTTGCCAGTCGCCTGTATTACAAGATGTTCGGCGGCACCCACCTGGT
GAACCGCGCGGGCCTGGCCCGGACGTTCCAGAACATTCGCCTGTTCAAGGAAATGTCGGTGCTGGAAAACCTGCTGGTGG
CCCAGCACATGTGGGTCAACCGCAACATGCTGGCCGGCATCCTCAACACCAAGGGCTATCGCAAGGCCGAAAGTGATGCG
CTGGACTGCGCGTTCTACTGGCTGGAAGTGGTGGATCTGGTGGACTGCGCCAACCGTCTGGCCGGTGAACTGTCCTACGG
CCAGCAACGCCGTCTGGAAATCGCCCGGGCCATGTGCACGCGGCCGCAGATCATCTGCCTCGACGAACCGGCCGCCGGCC
TCAACCCTCAGGAAACCGAAGCGCTGAGCGCGATGATCCGGCTGCTGCGCGACGAGCACGATCTGACCGTGGTGCTGATC
GAACACGACATGGGCATGGTAATGAGCATTTCCGATCACATCGTGGTGCTGGACCACGGCATCGTCATCGCCGAGGGCGG
ACCGGATGCCATCCGTAACGATCCGAAAGTGATCGCTGCTTATCTGGGCGCCGACGAAGAGGAAGTCGTATGA

Upstream 100 bases:

>100_bases
TGGTGTTGATGATGATCTGGCGACCTCGCGGGCTGATCCGCATCAGCCGTACCGGGGTCACTCCACGCAAAGGTGCCATT
CACTATGAGAGGACTGCGCC

Downstream 100 bases:

>100_bases
CGCAACCCATCCTCGAACTCAAGGATCTGGACGTGTTCTACGGCCCGATCCAGGCCCTCAAAGGCGTTTCGCTGCAGATC
AACGAAGGAGAAACCGTCAG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSEVVLSVEKLMMHFGGIKALSDVSLKVKRNSIFALIGPNGAGKTTVFNCLTGFYKASGGKIELNVRGQQTNVIQLLGES
FKPTDFVSPKNFASRLYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVLENLLVAQHMWVNRNMLAGILNTKGYRKAESDA
LDCAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQIICLDEPAAGLNPQETEALSAMIRLLRDEHDLTVVLI
EHDMGMVMSISDHIVVLDHGIVIAEGGPDAIRNDPKVIAAYLGADEEEVV

Sequences:

>Translated_290_residues
MSEVVLSVEKLMMHFGGIKALSDVSLKVKRNSIFALIGPNGAGKTTVFNCLTGFYKASGGKIELNVRGQQTNVIQLLGES
FKPTDFVSPKNFASRLYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVLENLLVAQHMWVNRNMLAGILNTKGYRKAESDA
LDCAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQIICLDEPAAGLNPQETEALSAMIRLLRDEHDLTVVLI
EHDMGMVMSISDHIVVLDHGIVIAEGGPDAIRNDPKVIAAYLGADEEEVV
>Mature_289_residues
SEVVLSVEKLMMHFGGIKALSDVSLKVKRNSIFALIGPNGAGKTTVFNCLTGFYKASGGKIELNVRGQQTNVIQLLGESF
KPTDFVSPKNFASRLYYKMFGGTHLVNRAGLARTFQNIRLFKEMSVLENLLVAQHMWVNRNMLAGILNTKGYRKAESDAL
DCAFYWLEVVDLVDCANRLAGELSYGQQRRLEIARAMCTRPQIICLDEPAAGLNPQETEALSAMIRLLRDEHDLTVVLIE
HDMGMVMSISDHIVVLDHGIVIAEGGPDAIRNDPKVIAAYLGADEEEVV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1789864, Length=283, Percent_Identity=49.469964664311, Blast_Score=273, Evalue=8e-75,
Organism=Escherichia coli, GI1789672, Length=273, Percent_Identity=25.6410256410256, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082268, Length=286, Percent_Identity=29.020979020979, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787029, Length=265, Percent_Identity=27.5471698113208, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788761, Length=282, Percent_Identity=27.3049645390071, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786872, Length=266, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI48995001, Length=256, Percent_Identity=30.46875, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790190, Length=263, Percent_Identity=30.4182509505703, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788225, Length=279, Percent_Identity=27.2401433691756, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787712, Length=271, Percent_Identity=27.3062730627306, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789593, Length=281, Percent_Identity=27.0462633451957, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788472, Length=285, Percent_Identity=30.8771929824561, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081709, Length=271, Percent_Identity=23.9852398523985, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=279, Percent_Identity=29.3906810035842, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789991, Length=256, Percent_Identity=27.34375, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790544, Length=279, Percent_Identity=27.5985663082437, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789032, Length=271, Percent_Identity=26.9372693726937, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788210, Length=263, Percent_Identity=28.8973384030418, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081791, Length=282, Percent_Identity=24.113475177305, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788450, Length=276, Percent_Identity=25.7246376811594, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787370, Length=271, Percent_Identity=24.3542435424354, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994997, Length=278, Percent_Identity=26.2589928057554, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787089, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790162, Length=277, Percent_Identity=27.4368231046931, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789586, Length=255, Percent_Identity=29.0196078431373, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786398, Length=260, Percent_Identity=25.3846153846154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786703, Length=224, Percent_Identity=27.2321428571429, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787758, Length=286, Percent_Identity=19.9300699300699, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786345, Length=270, Percent_Identity=24.4444444444444, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693107, Length=279, Percent_Identity=23.2974910394265, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790467, Length=276, Percent_Identity=23.5507246376812, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788165, Length=99, Percent_Identity=35.3535353535354, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787500, Length=289, Percent_Identity=23.1833910034602, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788506, Length=92, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786981, Length=238, Percent_Identity=28.9915966386555, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32074; Mature: 31942

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVLSVEKLMMHFGGIKALSDVSLKVKRNSIFALIGPNGAGKTTVFNCLTGFYKASGG
CCHHHHHHHHHHHHHCCCCHHCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KIELNVRGQQTNVIQLLGESFKPTDFVSPKNFASRLYYKMFGGTHLVNRAGLARTFQNIR
EEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFKEMSVLENLLVAQHMWVNRNMLAGILNTKGYRKAESDALDCAFYWLEVVDLVDCANRL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGELSYGQQRRLEIARAMCTRPQIICLDEPAAGLNPQETEALSAMIRLLRDEHDLTVVLI
HHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
EHDMGMVMSISDHIVVLDHGIVIAEGGPDAIRNDPKVIAAYLGADEEEVV
ECCCCEEEEECCCEEEEECCEEEECCCCHHHCCCHHEEEEECCCCHHCCC
>Mature Secondary Structure 
SEVVLSVEKLMMHFGGIKALSDVSLKVKRNSIFALIGPNGAGKTTVFNCLTGFYKASGG
CHHHHHHHHHHHHHCCCCHHCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KIELNVRGQQTNVIQLLGESFKPTDFVSPKNFASRLYYKMFGGTHLVNRAGLARTFQNIR
EEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFKEMSVLENLLVAQHMWVNRNMLAGILNTKGYRKAESDALDCAFYWLEVVDLVDCANRL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGELSYGQQRRLEIARAMCTRPQIICLDEPAAGLNPQETEALSAMIRLLRDEHDLTVVLI
HHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
EHDMGMVMSISDHIVVLDHGIVIAEGGPDAIRNDPKVIAAYLGADEEEVV
ECCCCEEEEECCCEEEEECCEEEECCCCHHHCCCHHEEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]