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The map label for this gene is braE [H]

Identifier: 77456782

GI number: 77456782

Start: 648025

End: 649326

Strand: Direct

Name: braE [H]

Synonym: Pfl01_0554

Alternate gene names: 77456782

Gene position: 648025-649326 (Clockwise)

Preceding gene: 77456781

Following gene: 77456783

Centisome position: 10.06

GC content: 61.21

Gene sequence:

>1302_bases
ATGTCTTCAACCACTACAAAATCCATTGATGTCAAAAAAAGCCTGGTTGAGGCGATTCTCGCCGGACTGATCGCCCTGAT
CGTGTTCGGGCCGATTGTCGGCGTGGTACTCGACGGCTACAGCTTCAACCTCGAACCGACCCGCGTGGCGTGGATCATCG
CCATCGTCATGGCCGGCCGTTTTGCCCTCAGCCTGTTCCTGCAAACCCCCAAAGGCCTGCGCATTCTCGACGGATTCGAG
AGCACCGGTTCCGGCGTGCATGTGCTGCCCCCGGACTACAAATCGCGCCTGCGCTGGATCATCCCGCTGATGATCGCCAT
CGCCGTAGCGTTGCCGTTCTTCTCCAACTCCTACCTGCTGGGTGTGGTCATCCTCGGACTGATCTACGTGCTGTTGGGGC
TGGGACTGAACATCGTGGTGGGTCTGGCCGGCCTGCTCGACCTGGGTTACGTGGCGTTCTACGCCATCGGTGCCTACGGT
CTGGCGCTCGGGTATCAGTACCTCGGGCTGGGCTTCTGGACCGTGCTGCCGCTCGCAGCGATCACCGCTGGCCTTGCCGG
TTGCATCCTCGGTTTCCCGGTGCTGCGCCTGCACGGCGACTACCTGGCGATCGTGACCCTGGGTTTCGGTGAAATCATTC
GTCTGGTGCTCAACAACTGGCTGTCCCTGACCGGCGGCCCGAACGGCATGCCGGCGCCACTGCCGACCTTCTTCGGTCTG
GAATTCGGCAAGCGGGCGAAGGATGGCGGGGTGCCGTTCCACGAGTTCTTCGGCATCGCCTACAACCCGGACGTGAAGTA
CTACTTCATTTATGCGGTGCTGTTCCTGGTGGTGCTGGCGGTGCTGTACATCAAGCATCGTCTGGTGAAGATGCCGGTTG
GCCGCGCTTGGGAAGCCTTGCGTGAAGACGAGATCGCCTGCCGCTCGATGGGCCTCAACCACGTACTGGTCAAGCTCTCG
GCGTTCACTATCGGTGCGTCCACCGCCGGTCTGGCGGGTGTGTTCTTCGCGACTTATCAAGGCTTCGTCAACCCGACCTC
GTTCACCTTCTTCGAATCGGCGCTGATCCTCGCCATCGTGGTGCTCGGCGGCATGGGCTCGACCATCGGTGTGGTGATTG
CAGCGTTCGTGCTGACGGTTGCTCCGGAACTGCTGCGCGGCTTCGCCGAATACCGCGTGCTGCTGTTCGGCATCCTGATG
GTGTTGATGATGATCTGGCGACCTCGCGGGCTGATCCGCATCAGCCGTACCGGGGTCACTCCACGCAAAGGTGCCATTCA
CTATGAGAGGACTGCGCCATGA

Upstream 100 bases:

>100_bases
CGACTACAAAGACGTGTTCAGCTTCTCGCTGCTCGTACTTGTTCTGGTCTTCCGGCCTCAGGGCCTGCTGGGCCGTCCTC
TTGTGTCGAAGGTGTAAGCG

Downstream 100 bases:

>100_bases
GTGAGGTCGTACTCTCTGTTGAAAAACTGATGATGCACTTCGGTGGCATCAAGGCCTTGAGCGACGTCAGCCTGAAGGTC
AAACGCAACTCGATCTTCGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSSTTTKSIDVKKSLVEAILAGLIALIVFGPIVGVVLDGYSFNLEPTRVAWIIAIVMAGRFALSLFLQTPKGLRILDGFE
STGSGVHVLPPDYKSRLRWIIPLMIAIAVALPFFSNSYLLGVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYG
LALGYQYLGLGFWTVLPLAAITAGLAGCILGFPVLRLHGDYLAIVTLGFGEIIRLVLNNWLSLTGGPNGMPAPLPTFFGL
EFGKRAKDGGVPFHEFFGIAYNPDVKYYFIYAVLFLVVLAVLYIKHRLVKMPVGRAWEALREDEIACRSMGLNHVLVKLS
AFTIGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVIAAFVLTVAPELLRGFAEYRVLLFGILM
VLMMIWRPRGLIRISRTGVTPRKGAIHYERTAP

Sequences:

>Translated_433_residues
MSSTTTKSIDVKKSLVEAILAGLIALIVFGPIVGVVLDGYSFNLEPTRVAWIIAIVMAGRFALSLFLQTPKGLRILDGFE
STGSGVHVLPPDYKSRLRWIIPLMIAIAVALPFFSNSYLLGVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYG
LALGYQYLGLGFWTVLPLAAITAGLAGCILGFPVLRLHGDYLAIVTLGFGEIIRLVLNNWLSLTGGPNGMPAPLPTFFGL
EFGKRAKDGGVPFHEFFGIAYNPDVKYYFIYAVLFLVVLAVLYIKHRLVKMPVGRAWEALREDEIACRSMGLNHVLVKLS
AFTIGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVIAAFVLTVAPELLRGFAEYRVLLFGILM
VLMMIWRPRGLIRISRTGVTPRKGAIHYERTAP
>Mature_432_residues
SSTTTKSIDVKKSLVEAILAGLIALIVFGPIVGVVLDGYSFNLEPTRVAWIIAIVMAGRFALSLFLQTPKGLRILDGFES
TGSGVHVLPPDYKSRLRWIIPLMIAIAVALPFFSNSYLLGVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGL
ALGYQYLGLGFWTVLPLAAITAGLAGCILGFPVLRLHGDYLAIVTLGFGEIIRLVLNNWLSLTGGPNGMPAPLPTFFGLE
FGKRAKDGGVPFHEFFGIAYNPDVKYYFIYAVLFLVVLAVLYIKHRLVKMPVGRAWEALREDEIACRSMGLNHVLVKLSA
FTIGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVIAAFVLTVAPELLRGFAEYRVLLFGILMV
LMMIWRPRGLIRISRTGVTPRKGAIHYERTAP

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=321, Percent_Identity=57.3208722741433, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46760; Mature: 46628

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCC
>Mature Secondary Structure 
SSTTTKSIDVKKSLVEAILAGLIALIVFGPIVGVVLDGYSFNLEPTRVAWIIAIVMAGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
FALSLFLQTPKGLRILDGFESTGSGVHVLPPDYKSRLRWIIPLMIAIAVALPFFSNSYLL
HHHHHHHCCCCCCEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
GVVILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLALGYQYLGLGFWTVLPLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITAGLAGCILGFPVLRLHGDYLAIVTLGFGEIIRLVLNNWLSLTGGPNGMPAPLPTFFGL
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
EFGKRAKDGGVPFHEFFGIAYNPDVKYYFIYAVLFLVVLAVLYIKHRLVKMPVGRAWEAL
HHCCCCCCCCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
REDEIACRSMGLNHVLVKLSAFTIGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIV
HHHHHHHHHCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VLGGMGSTIGVVIAAFVLTVAPELLRGFAEYRVLLFGILMVLMMIWRPRGLIRISRTGVT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PRKGAIHYERTAP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]