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The map label for this gene is dnaB [H]

Identifier: 77456766

GI number: 77456766

Start: 623970

End: 625367

Strand: Direct

Name: dnaB [H]

Synonym: Pfl01_0538

Alternate gene names: 77456766

Gene position: 623970-625367 (Clockwise)

Preceding gene: 77456765

Following gene: 77456768

Centisome position: 9.69

GC content: 61.16

Gene sequence:

>1398_bases
ATGAACGAAATCTCCGCTCCCGAGCAATACGATCTGCAAACCGCCGCGCTGAAGGTGCCGCCGCATTCCATCGAGGCCGA
ACAGGCCGTGCTCGGTGGTCTGATGCTGGACAACAACGCCTGGGAACGCGTGCTCGATCAAGTCTCCGACGGCGATTTCT
ACCGACATGACCACCGTCTGATTTTCCGTGCGATCGCCAAACTGGCCGATCAGAACCTGCCGATCGATGTCGTGACCCTT
GCCGAGCAACTGGACAAGGAAGGTCAGACTTCGCAGGTCGGCGGCCTCGGTTACCTGGGTGAGCTGGCGAAAAACACGCC
GTCCGTCGCCAACATCAAGGCCTATGCGCAGATCGTCCGCCAGCGCGCGACGTTGCGTCAGCTGATCGGTATCAGCACCG
AAATTGCCGACAGCGCCTTCAATCCCGAAGGCCGCACCGCCGAGGAAATCCTCGACGAAGCCGAGCGGCAGATCTTCGCG
ATTGCCGAGGCCCGGCCAAAAACCGGCGGCCCGGTGGGTGTGAATGACCTGCTGACCAAGGCCATCGACCGCATCGACAC
CCTGTTCAACACTGCCGACTCGATCACCGGCCTGTCCACCGGCTACACCGACCTCGACGAGAAGACCAGCGGCCTGCAAC
CGGCCGACCTGATCATCGTCGCCGGCCGTCCGTCGATGGGTAAGACCACCTTCGCGATGAACCTGGTGGAAAACGCCGTG
TTGCGCAGCGACAAAGTGGTGCTGGTGTACTCCCTCGAGATGCCAGGCGAATCGCTGATCATGCGTATGCTGTCGTCCCT
CGGCCGTATCGACCAGACCAAGGTACGTTCCGGCCAGTTGGAAGACGACGACTGGCCGCGTCTGACCTCGGCGGTCAATC
TGCTCAACGACCGCAAGCTGTTCATCGATGACACCGCCGGCATCAGTCCGTCGGAGATGCGTGCCCGGACCCGGCGGCTG
GTGCGCGAGCACGGCGACATCGGCCTGATCATGATCGACTACCTGCAGCTGATGCAGATCCCGGGTTCCAGCGGCGACAA
CCGGACCAACGAGATTTCCGAGATCTCCCGATCCCTGAAAGCCCTGGCCAAGGAATTCAACTGCCCGGTGGTCGCCCTGT
CGCAGCTCAACCGCTCCCTGGAACAGCGTCCCAACAAGCGCCCGGTGAACTCCGACTTGCGGGAATCCGGAGCGATCGAG
CAGGACGCCGACGTGATCATGTTCGTGTACCGGGATGAGGTGTATCACCCGGAAACCGAACACAAGGGCATCGCCGAAAT
CATCATCGGCAAACAGCGGAACGGTCCGATCGGCTTCATTCGCCTGGCTTTCATCGGCAAATACACACGTTTCGAAAACC
TCGCGCCGGGCAGTTACAACTTCGACGACGACGAGTAA

Upstream 100 bases:

>100_bases
CGGCTTGTCCGGCAGACGGTTAACATCGGGCACGATCCTGTTTACAGGTCGTGCCCTTTGTCTTTCTGCACTCCCTGCTT
TTCCTGATTAAAAAGTGGCC

Downstream 100 bases:

>100_bases
CGCAATTGATCACGTCAGCTGATCAAACAGTTTCTGGGCAACCCTGGGGCTGATGAACTCCCGCCATATCCGTTGGTGGG
GCCGACCAGCCTGTTCTTCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNEISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIAKLADQNLPIDVVTL
AEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVRQRATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFA
IAEARPKTGGPVGVNDLLTKAIDRIDTLFNTADSITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRSGQLEDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRL
VREHGDIGLIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVNSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGFIRLAFIGKYTRFENLAPGSYNFDDDE

Sequences:

>Translated_465_residues
MNEISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIAKLADQNLPIDVVTL
AEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVRQRATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFA
IAEARPKTGGPVGVNDLLTKAIDRIDTLFNTADSITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRSGQLEDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRL
VREHGDIGLIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVNSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGFIRLAFIGKYTRFENLAPGSYNFDDDE
>Mature_465_residues
MNEISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIAKLADQNLPIDVVTL
AEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVRQRATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFA
IAEARPKTGGPVGVNDLLTKAIDRIDTLFNTADSITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRSGQLEDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRL
VREHGDIGLIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVVALSQLNRSLEQRPNKRPVNSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGFIRLAFIGKYTRFENLAPGSYNFDDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=462, Percent_Identity=61.9047619047619, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51496; Mature: 51496

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRL
CCCCCCCCCCCCHHEEEECCCCCCCHHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHH
IFRAIAKLADQNLPIDVVTLAEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVR
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
QRATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFAIAEARPKTGGPVGVNDLLTK
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHEEHHHHCCCCCCCCCCHHHHHHH
AIDRIDTLFNTADSITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRSGQLEDDDWPRLTSAVNLLNDRKL
HCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEE
FIDDTAGISPSEMRARTRRLVREHGDIGLIMIDYLQLMQIPGSSGDNRTNEISEISRSLK
EEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ALAKEFNCPVVALSQLNRSLEQRPNKRPVNSDLRESGAIEQDADVIMFVYRDEVYHPETE
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCHH
HKGIAEIIIGKQRNGPIGFIRLAFIGKYTRFENLAPGSYNFDDDE
CCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNEISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRL
CCCCCCCCCCCCHHEEEECCCCCCCHHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHH
IFRAIAKLADQNLPIDVVTLAEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVR
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
QRATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFAIAEARPKTGGPVGVNDLLTK
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHEEHHHHCCCCCCCCCCHHHHHHH
AIDRIDTLFNTADSITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHH
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRSGQLEDDDWPRLTSAVNLLNDRKL
HCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCEE
FIDDTAGISPSEMRARTRRLVREHGDIGLIMIDYLQLMQIPGSSGDNRTNEISEISRSLK
EEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ALAKEFNCPVVALSQLNRSLEQRPNKRPVNSDLRESGAIEQDADVIMFVYRDEVYHPETE
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCHH
HKGIAEIIIGKQRNGPIGFIRLAFIGKYTRFENLAPGSYNFDDDE
CCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]