Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 77456756

GI number: 77456756

Start: 611314

End: 612603

Strand: Direct

Name: purA [H]

Synonym: Pfl01_0528

Alternate gene names: 77456756

Gene position: 611314-612603 (Clockwise)

Preceding gene: 77456755

Following gene: 77456757

Centisome position: 9.49

GC content: 61.78

Gene sequence:

>1290_bases
ATGGGTAAGAATGTCGTAGTCCTGGGCACCCAATGGGGTGATGAGGGCAAAGGCAAGATCGTTGATCTGCTGACCGAACA
TGCTGCCGCCGTAGTGCGCTACCAGGGTGGCCACAACGCCGGTCACACTCTGGTGATCGACGGTGAAAAAACCGTCTTGC
ACCTGATCCCGTCGGGCGTGCTGCGCGAAGGCGTGCAGTGCCTGATCGGCAACGGCGTGGTGGTTGCACCTGACGCCCTG
CTGCGCGAGATCACCAAGCTGGAAGAGAAAGGCGTACCGGTGCGCGAGCGCCTGCGTATCAGCCCTTCCTGCCCGCTGAT
CCTGTCCTTCCACGTGGCGCTGGACCAGGCCCGTGAAAAGGCCCGCGGCGAGCTGAAGATCGGTACGACCGGTCGCGGCA
TCGGCCCGGCTTACGAAGACAAGGTTGCACGTCGCGGCCTGCGTGTTGGCGATCTGCTCAACATGCCGCGCTTTGAAGAC
AAGCTGCGTGAACTGGTGGACTACCACAACTTCATGCTGGTCGGTTACTACAAAGAGCCTGCCATCGAGTTCGAAAAGAC
GCTGGCCGAGTGCAAGGAATACGCCGAGCTGCTCAAGCCGCTGATGCTGGACGTGACTGCCGAGCTGCACGACCTGCGTC
GCGCCGGCAAAGACATCATGTTCGAAGGCGCCCAGGGTTCGTTGCTCGACATCGACCACGGTACCTATCCGTACGTGACC
AGCTCCAACACCACCGCGGGTGGCGTTGCTACCGGCTCGGGCGTTGGCCCGATGTTCCTGGACTACATCCTGGGCATCAC
CAAGGCTTACACCACTCGCGTAGGTTCGGGCCCATTCCCGACCGAGCTGTTCGACGAAGTCGGTGCGCACCTGGCCAAGC
AAGGTCACGAGTTCGGCGCTACCACCGGCCGTGCCCGTCGTTGCGGCTGGTTCGACGCCGTTATCCTGCGTCGTGCTATC
GATGTGAACAGCATCTCGGGCATCTGCCTGACCAAGCTGGACGTACTCGACGGCCTGGAAACCATCAACATCTGCACCGG
CTACAAGGATGCGCAAGGCAACGCGGTTGCCCCGACTGACGCTGACAGCTACGTGGGCCTGCAGCCTGTGTACGAAGAAG
TGCCGGGCTGGAGCGAATCGACCGTGGGCGCCAAGACTCTGGAAGAGCTGCCGGCCAACGCTCGCGCCTACATCAAGCGC
GTTGAAGAGTTGATCGGCGCGCCGATCGACATTATTTCGACGGGCCCGGACCGCAACGAAACCATCGTGCTGCGTCATCC
GTTCGCTTGA

Upstream 100 bases:

>100_bases
GATCCAGCAGAACGGGCTTTGGCAAGTATCGCCACTGGCTTCTTGAGTTTTCCTGCCGGCTGATGCCGGCACCAAGTTTG
CGCGAATGAGGACAAGTGTT

Downstream 100 bases:

>100_bases
TAAGTCGTTGATGTAAAAACACAAAGGCCCCTTCATCGGGGCCTTTGTCGTTTATGCCTGTTGGACGGCATGACCTTTGC
TGTGATTCTGTCTACAAGAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDAL
LREITKLEEKGVPVRERLRISPSCPLILSFHVALDQAREKARGELKIGTTGRGIGPAYEDKVARRGLRVGDLLNMPRFED
KLRELVDYHNFMLVGYYKEPAIEFEKTLAECKEYAELLKPLMLDVTAELHDLRRAGKDIMFEGAQGSLLDIDHGTYPYVT
SSNTTAGGVATGSGVGPMFLDYILGITKAYTTRVGSGPFPTELFDEVGAHLAKQGHEFGATTGRARRCGWFDAVILRRAI
DVNSISGICLTKLDVLDGLETINICTGYKDAQGNAVAPTDADSYVGLQPVYEEVPGWSESTVGAKTLEELPANARAYIKR
VEELIGAPIDIISTGPDRNETIVLRHPFA

Sequences:

>Translated_429_residues
MGKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDAL
LREITKLEEKGVPVRERLRISPSCPLILSFHVALDQAREKARGELKIGTTGRGIGPAYEDKVARRGLRVGDLLNMPRFED
KLRELVDYHNFMLVGYYKEPAIEFEKTLAECKEYAELLKPLMLDVTAELHDLRRAGKDIMFEGAQGSLLDIDHGTYPYVT
SSNTTAGGVATGSGVGPMFLDYILGITKAYTTRVGSGPFPTELFDEVGAHLAKQGHEFGATTGRARRCGWFDAVILRRAI
DVNSISGICLTKLDVLDGLETINICTGYKDAQGNAVAPTDADSYVGLQPVYEEVPGWSESTVGAKTLEELPANARAYIKR
VEELIGAPIDIISTGPDRNETIVLRHPFA
>Mature_428_residues
GKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGVLREGVQCLIGNGVVVAPDALL
REITKLEEKGVPVRERLRISPSCPLILSFHVALDQAREKARGELKIGTTGRGIGPAYEDKVARRGLRVGDLLNMPRFEDK
LRELVDYHNFMLVGYYKEPAIEFEKTLAECKEYAELLKPLMLDVTAELHDLRRAGKDIMFEGAQGSLLDIDHGTYPYVTS
SNTTAGGVATGSGVGPMFLDYILGITKAYTTRVGSGPFPTELFDEVGAHLAKQGHEFGATTGRARRCGWFDAVILRRAID
VNSISGICLTKLDVLDGLETINICTGYKDAQGNAVAPTDADSYVGLQPVYEEVPGWSESTVGAKTLEELPANARAYIKRV
EELIGAPIDIISTGPDRNETIVLRHPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.4272300469484, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.0560747663551, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=41.5617128463476, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=66.7441860465116, Blast_Score=601, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=45.0346420323326, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=45.0346420323326, Blast_Score=352, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=43.0232558139535, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=43.0588235294118, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46546; Mature: 46415

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LREGVQCLIGNGVVVAPDALLREITKLEEKGVPVRERLRISPSCPLILSFHVALDQAREK
HHHHHHEEECCCEEECCHHHHHHHHHHHHCCCCHHHHEECCCCCCEEEHHHHHHHHHHHH
ARGELKIGTTGRGIGPAYEDKVARRGLRVGDLLNMPRFEDKLRELVDYHNFMLVGYYKEP
HCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCC
AIEFEKTLAECKEYAELLKPLMLDVTAELHDLRRAGKDIMFEGAQGSLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGVGPMFLDYILGITKAYTTRVGSGPFPTELFDEVGAHLAKQGHEFGA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVILRRAIDVNSISGICLTKLDVLDGLETINICTGYKDAQGNAVAPTD
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEHCCCCCCCCCCEECCCC
ADSYVGLQPVYEEVPGWSESTVGAKTLEELPANARAYIKRVEELIGAPIDIISTGPDRNE
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
TIVLRHPFA
EEEEECCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTEHAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGV
CCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LREGVQCLIGNGVVVAPDALLREITKLEEKGVPVRERLRISPSCPLILSFHVALDQAREK
HHHHHHEEECCCEEECCHHHHHHHHHHHHCCCCHHHHEECCCCCCEEEHHHHHHHHHHHH
ARGELKIGTTGRGIGPAYEDKVARRGLRVGDLLNMPRFEDKLRELVDYHNFMLVGYYKEP
HCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCC
AIEFEKTLAECKEYAELLKPLMLDVTAELHDLRRAGKDIMFEGAQGSLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGVGPMFLDYILGITKAYTTRVGSGPFPTELFDEVGAHLAKQGHEFGA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRARRCGWFDAVILRRAIDVNSISGICLTKLDVLDGLETINICTGYKDAQGNAVAPTD
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEHCCCCCCCCCCEECCCC
ADSYVGLQPVYEEVPGWSESTVGAKTLEELPANARAYIKRVEELIGAPIDIISTGPDRNE
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
TIVLRHPFA
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA