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The map label for this gene is miaA [H]

Identifier: 77456750

GI number: 77456750

Start: 604984

End: 605955

Strand: Direct

Name: miaA [H]

Synonym: Pfl01_0522

Alternate gene names: 77456750

Gene position: 604984-605955 (Clockwise)

Preceding gene: 77456749

Following gene: 77456751

Centisome position: 9.4

GC content: 58.13

Gene sequence:

>972_bases
ATGAGCCAGCTTCCTCCAGCGATTTTCCTGATGGGCCCGACCGCTGCGGGCAAGACCGATCTGGCCATCGAGCTGACCAA
GGTGCTGCCGTGCGAGCTGATCAGCGTTGACTCGGCGCTGGTCTATCGTGGCATGGACATCGGCACCGCCAAGCCTTCCA
AAGAGCTGCTGGCCGAATTTCCCCACCGCTTGATCGACATTCTCGACCCGGCCGAAGCGTATTCGGCAGCGGATTTCCGC
CGTGATGCGCTGGAGGCCATGGCCGAGATCACCGCACGTGGAAAAATTCCACTGCTGGTGGGCGGCACGATGCTCTATTA
CAAGGCTTTGATCGAAGGTCTGGCCGATATGCCGGCGGCCGATCCCGAAGTTCGTGCGCAGATCGAAGAAGAGGCTGCAC
GCCTTGGCTGGCAGGCCCTGCACGATCAACTGGCAGCAATCGATCCGGAATCGGCGGCGCGCATCCATCCGAACGATCCG
CAACGACTGAGTCGTGCGCTGGAAGTTTACCGGGTCAGCGGTCAAAGCATGACTGAACTGCGGCTGAAACAATCTGTACA
AAGTACCGAAGCGGCCGCATCGGGACTGCAACAATTGCCCTATACTGTCGCGAACCTGGCGATTGCTCCAACTAATCGGC
AGGTACTGCACGAGCGCATTAAACAAAGATTCACAAATATGCTGGAACAGGGATTCATCGACGAGGTCGTAGCCCTGCGT
AATAGAAGTGACCTGCATGCCGGGTTGCCGTCTATACGTGCGGTAGGCTACCGCCAGGTCTGGGACTTCCTGGATGGCAA
GCTGACGTCGGCCGAGATGCAGGAACGGGGCATCATCGCCACGCGCCAATTGGCCAAACGCCAGTTCACCTGGCTGCGCA
GCTGGAAAGATTTACACTGGCTCGACAGTCTGGATTGCGACAATCTGCCACGCGCCTTGAAATACCTTGGGACGATCTCC
ATATTGAGCTGA

Upstream 100 bases:

>100_bases
TGGAAAACACCGAGCGCAGCGGTCAATGCAACCATGGCCGACCGACCTGGACCCAACTGGGCCTGGACGATCTGGACAAA
CTGTTCCTGCGCGGTCGTTG

Downstream 100 bases:

>100_bases
GTCCTTGCAATTGCCGTCTATCCTTGGGGGTGTGGCGGCAAAAGCCATCTGTTTACCTATTTTTTATATTGAATCCTTAA
AGGAGTGCGGCACATGTCAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAEFPHRLIDILDPAEAYSAADFR
RDALEAMAEITARGKIPLLVGGTMLYYKALIEGLADMPAADPEVRAQIEEEAARLGWQALHDQLAAIDPESAARIHPNDP
QRLSRALEVYRVSGQSMTELRLKQSVQSTEAAASGLQQLPYTVANLAIAPTNRQVLHERIKQRFTNMLEQGFIDEVVALR
NRSDLHAGLPSIRAVGYRQVWDFLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWKDLHWLDSLDCDNLPRALKYLGTIS
ILS

Sequences:

>Translated_323_residues
MSQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAEFPHRLIDILDPAEAYSAADFR
RDALEAMAEITARGKIPLLVGGTMLYYKALIEGLADMPAADPEVRAQIEEEAARLGWQALHDQLAAIDPESAARIHPNDP
QRLSRALEVYRVSGQSMTELRLKQSVQSTEAAASGLQQLPYTVANLAIAPTNRQVLHERIKQRFTNMLEQGFIDEVVALR
NRSDLHAGLPSIRAVGYRQVWDFLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWKDLHWLDSLDCDNLPRALKYLGTIS
ILS
>Mature_322_residues
SQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAEFPHRLIDILDPAEAYSAADFRR
DALEAMAEITARGKIPLLVGGTMLYYKALIEGLADMPAADPEVRAQIEEEAARLGWQALHDQLAAIDPESAARIHPNDPQ
RLSRALEVYRVSGQSMTELRLKQSVQSTEAAASGLQQLPYTVANLAIAPTNRQVLHERIKQRFTNMLEQGFIDEVVALRN
RSDLHAGLPSIRAVGYRQVWDFLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWKDLHWLDSLDCDNLPRALKYLGTISI
LS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=28.9808917197452, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=64.5695364238411, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25144712, Length=247, Percent_Identity=31.9838056680162, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=26.4214046822742, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24649830, Length=354, Percent_Identity=29.3785310734463, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35881; Mature: 35750

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAEF
CCCCCCEEEEECCCCCCCCCHHEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
PHRLIDILDPAEAYSAADFRRDALEAMAEITARGKIPLLVGGTMLYYKALIEGLADMPAA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCC
DPEVRAQIEEEAARLGWQALHDQLAAIDPESAARIHPNDPQRLSRALEVYRVSGQSMTEL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
RLKQSVQSTEAAASGLQQLPYTVANLAIAPTNRQVLHERIKQRFTNMLEQGFIDEVVALR
HHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRSDLHAGLPSIRAVGYRQVWDFLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWKDLHW
CCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LDSLDCDNLPRALKYLGTISILS
HCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAEF
CCCCCEEEEECCCCCCCCCHHEEHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
PHRLIDILDPAEAYSAADFRRDALEAMAEITARGKIPLLVGGTMLYYKALIEGLADMPAA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCC
DPEVRAQIEEEAARLGWQALHDQLAAIDPESAARIHPNDPQRLSRALEVYRVSGQSMTEL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
RLKQSVQSTEAAASGLQQLPYTVANLAIAPTNRQVLHERIKQRFTNMLEQGFIDEVVALR
HHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRSDLHAGLPSIRAVGYRQVWDFLDGKLTSAEMQERGIIATRQLAKRQFTWLRSWKDLHW
CCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LDSLDCDNLPRALKYLGTISILS
HCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA