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The map label for this gene is amiC [H]

Identifier: 77456748

GI number: 77456748

Start: 601659

End: 603080

Strand: Direct

Name: amiC [H]

Synonym: Pfl01_0520

Alternate gene names: 77456748

Gene position: 601659-603080 (Clockwise)

Preceding gene: 77456747

Following gene: 77456749

Centisome position: 9.34

GC content: 64.14

Gene sequence:

>1422_bases
ATGCGCTTTCGCGCGTTGGTGGCTGCCGCTGGACTGATGTTGATGGCAGTAACCGTCAACGCTGTGGCCGAGACGAAGGT
CAACAGCGTGCGCCTGTGGCGGGCGCCGGACAACACGCGACTGGTGTTCGACCTGACAGGCCCGGTGCAGCACAGCGTCT
TCACGCTGACCGCGCCGGATCGTCTGGTGATCGATATCAACGGCGCCACCCTCGGCGCGCCGCTGAATGTGCAGACCGCC
AACACGCCGATTACCGCCATGCGTTCGGCCCAGCGCACGCCGACCGACCTGCGGGTGGTCATCGACCTGAAAAAAGCCGT
CACTCCGAAAAGCTTCTCGCTGGCGCCGAATGCGCAGTATGGCAACCGTCTGGTGGTCGACCTGTTCGATAACCCGGCTG
ACGCCGCACCAGCGCCTACGCCTGCGTCGACCCCAACGGTTGCGACCGTTCCTGCGGTGCCGGTGACGCCGGCCGAGCCT
GCGATCAAACTGCCACCAGCCCCGGCCGGCAAACGCGACATCATCGTGGTCATCGACGCCGGCCACGGTGGCGAAGACCC
GGGTGCATCGGGCTCGCGCGGTCAGCGCGAAAAAGACGTGGTACTGCAGATCGCCCGTGAGCTGCAGCGTCAGGTCAACG
GCATGAAAGGCTTCCGCGCCGAACTGACCCGTACCGGTGACTATTTCATCCCGCTGCGCGGCCGTACCGAGATTGCTCGC
AAGAAGGGCGCCGACCTGTTCGTCTCGATCCACGCCGACGCCGCGCCGTCAGCGGCCGCGTTCGGTGCGTCGGTGTTCGC
CCTGTCCGACCGTGGTGCCACTTCGGAAACCGCCCGTTGGCTGGCCGACAGCGAAAACCGTTCCGACCTGATCGGCGGTG
CCGGCAACGTCAGTCTCGATGACAAGGACCGGATGCTGGCCGGTGTTCTGCTCGACCTGTCGATGACCGCCTCGCTGACC
TCCAGCCTCAACGTCGGCCAGAAAGTCCTGACCAACATTGGCCGGGTCACCCCGCTGCACAAACAGCGCGTGGAACAGGC
CGGGTTCATGGTGCTGAAATCGCCTGACATCCCGTCGATCCTGGTGGAAACCGGCTTCATCTCCAACGCCAACGAAGCGA
ACAAGCTTTCGGCAGCGAGCCACCAGCAGGCGCTGGCGCGTTCGATCAGCAGCGGCGTGCGCCAGTTCTTCCAGCAGAAT
CCGCCACCGGGCACTTACATTGCCTGGCTGCGCGATTCGGGCAAGATCGCTCAAGGGCCGCGTGACCACCGGGTAGGTCC
GGGTGAGACCGTGGCAATGATTGCCGTGCGTTATCAGATTTCGCCGGCCACGCTGCGCAGCGCCAACAATCTGAAAAGCG
ATGATCTGAAAGTCGGTCAGCACCTGACCATTCCTGGCACCGAACTGGCGTCCAAAGAATGA

Upstream 100 bases:

>100_bases
AGACAGCGGACGTTCGCTGACCATTTTATCCCAGGGCTCGCGCGGCGAGGCCTGGTGTGCCGCTTTGGCATTGGAAACCA
ATTGAATGATGGGGTTAGGT

Downstream 100 bases:

>100_bases
ATCAGGTCCTGAACGCTGCCCGCATCGAACTGCTCAGCCCGCGGCTGGCGAACCAGATCGCCGCCGGTGAGGTGGTCGAA
CGCCCGGCCTCGGTGATCAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRFRALVAAAGLMLMAVTVNAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFTLTAPDRLVIDINGATLGAPLNVQTA
NTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFSLAPNAQYGNRLVVDLFDNPADAAPAPTPASTPTVATVPAVPVTPAEP
AIKLPPAPAGKRDIIVVIDAGHGGEDPGASGSRGQREKDVVLQIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEIAR
KKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVSLDDKDRMLAGVLLDLSMTASLT
SSLNVGQKVLTNIGRVTPLHKQRVEQAGFMVLKSPDIPSILVETGFISNANEANKLSAASHQQALARSISSGVRQFFQQN
PPPGTYIAWLRDSGKIAQGPRDHRVGPGETVAMIAVRYQISPATLRSANNLKSDDLKVGQHLTIPGTELASKE

Sequences:

>Translated_473_residues
MRFRALVAAAGLMLMAVTVNAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFTLTAPDRLVIDINGATLGAPLNVQTA
NTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFSLAPNAQYGNRLVVDLFDNPADAAPAPTPASTPTVATVPAVPVTPAEP
AIKLPPAPAGKRDIIVVIDAGHGGEDPGASGSRGQREKDVVLQIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEIAR
KKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVSLDDKDRMLAGVLLDLSMTASLT
SSLNVGQKVLTNIGRVTPLHKQRVEQAGFMVLKSPDIPSILVETGFISNANEANKLSAASHQQALARSISSGVRQFFQQN
PPPGTYIAWLRDSGKIAQGPRDHRVGPGETVAMIAVRYQISPATLRSANNLKSDDLKVGQHLTIPGTELASKE
>Mature_473_residues
MRFRALVAAAGLMLMAVTVNAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFTLTAPDRLVIDINGATLGAPLNVQTA
NTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFSLAPNAQYGNRLVVDLFDNPADAAPAPTPASTPTVATVPAVPVTPAEP
AIKLPPAPAGKRDIIVVIDAGHGGEDPGASGSRGQREKDVVLQIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEIAR
KKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVSLDDKDRMLAGVLLDLSMTASLT
SSLNVGQKVLTNIGRVTPLHKQRVEQAGFMVLKSPDIPSILVETGFISNANEANKLSAASHQQALARSISSGVRQFFQQN
PPPGTYIAWLRDSGKIAQGPRDHRVGPGETVAMIAVRYQISPATLRSANNLKSDDLKVGQHLTIPGTELASKE

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=410, Percent_Identity=40.7317073170732, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1790611, Length=421, Percent_Identity=35.8669833729216, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788776, Length=229, Percent_Identity=49.3449781659389, Blast_Score=205, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 50149; Mature: 50149

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFRALVAAAGLMLMAVTVNAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFTLTAPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEECCC
RLVIDINGATLGAPLNVQTANTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFSLAPNAQY
EEEEEECCCEECCCEEEEECCCCHHHHHHCCCCCCCEEEEEEEHHHCCCCCEECCCCCCC
GNRLVVDLFDNPADAAPAPTPASTPTVATVPAVPVTPAEPAIKLPPAPAGKRDIIVVIDA
CCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCCCCCCCCEEEEEEC
GHGGEDPGASGSRGQREKDVVLQIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEIAR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCHHHHH
KKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVSLD
HCCCEEEEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCHHCCCCCCCCCC
DKDRMLAGVLLDLSMTASLTSSLNVGQKVLTNIGRVTPLHKQRVEQAGFMVLKSPDIPSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECCCCCEE
LVETGFISNANEANKLSAASHQQALARSISSGVRQFFQQNPPPGTYIAWLRDSGKIAQGP
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RDHRVGPGETVAMIAVRYQISPATLRSANNLKSDDLKVGQHLTIPGTELASKE
CCCCCCCCCEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCC
>Mature Secondary Structure
MRFRALVAAAGLMLMAVTVNAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFTLTAPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEECCC
RLVIDINGATLGAPLNVQTANTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFSLAPNAQY
EEEEEECCCEECCCEEEEECCCCHHHHHHCCCCCCCEEEEEEEHHHCCCCCEECCCCCCC
GNRLVVDLFDNPADAAPAPTPASTPTVATVPAVPVTPAEPAIKLPPAPAGKRDIIVVIDA
CCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCCCCCCCCEEEEEEC
GHGGEDPGASGSRGQREKDVVLQIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEIAR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCHHHHH
KKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVSLD
HCCCEEEEEEECCCCCCHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCHHCCCCCCCCCC
DKDRMLAGVLLDLSMTASLTSSLNVGQKVLTNIGRVTPLHKQRVEQAGFMVLKSPDIPSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECCCCCEE
LVETGFISNANEANKLSAASHQQALARSISSGVRQFFQQNPPPGTYIAWLRDSGKIAQGP
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RDHRVGPGETVAMIAVRYQISPATLRSANNLKSDDLKVGQHLTIPGTELASKE
CCCCCCCCCEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]