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The map label for this gene is rhdA [H]

Identifier: 77456738

GI number: 77456738

Start: 591646

End: 592461

Strand: Reverse

Name: rhdA [H]

Synonym: Pfl01_0510

Alternate gene names: 77456738

Gene position: 592461-591646 (Counterclockwise)

Preceding gene: 77456739

Following gene: 77456737

Centisome position: 9.2

GC content: 63.73

Gene sequence:

>816_bases
ATGTCTGATTTCTCTGGCCTGGCCCTGGTGATCGAGCCGAGCGATCTGCTGCCGCGCCTCGAATCCCGCGATCTGATTCT
GGTGGATCTGACCAGTGCCGCCCGCTACGCCGAAGGACACATTCCCGGCGCACGTTTTGTCGATCCGAAACGCACCCAGC
TGGGTCAGGCGCCGGCTCCGGGGCTGTTGCCGAGCAAGGAAAAACTCGAAGAACTGTTCGGTGAGCTGGGCCATCGCAAA
GATGCGGTTTACGTGGTCTATGACGACGAAGGCGGCGGCTGGGCCGGGCGCTTCATCTGGCTGCTCGATGTGATCGGTCA
CGACCAATACCACTACCTCAACGGCGGCCTGCCGGCGTGGCTGGCGGAGGGTTCACCGATGTCGATCCAGATTCCTCCGG
CGGTGGGCGGCCCGGTTGCCTTGACCCTGCACGAGGAACCGACCGCCACCCGCGAATACCTGCAAAGCCGTCTCGGCGCC
GCTGACCTGGCGATCTGGGATGCTCGCGGGCCGCTGGAGTATTCCGGTGAGAAGGTCCTGGCGGCCAAGGGCGGGCACAT
CCCCGGCGCGGTCAATTTCGAATGGACGGCGGGCATGGATCAGGCGCGCCAACTGCGCATCCGTACCGACATGCCGCAGA
TCCTCGAAGACCTCGGGATCACCAGGGACAAAGAAATCATTACCCACTGCCAGACCCATCACCGGTCCGGTTTCACTTAT
CTGGTGGCCAAGGCCCTCGGTTATCCGCGGGTCAAGGGCTACGCCGGTTCCTGGGGCGAATGGGGCAACCACCCCGACAC
CCCCGTCGAGCTTTAA

Upstream 100 bases:

>100_bases
CGGCAAAACCGCGCAGTGCATCGAGAAAGCCCTGCACAGTTTTAGCCAGCGCGGCCAATGACAGCTACAATCCCTCCCCT
TTGTGCTCTGGAGACCTCAC

Downstream 100 bases:

>100_bases
GGATTTTTAAGGACAGTCATGAAAGAGCGTTTGTTTATCATCAGCCAGTACCTGCTGCCGCATCACTTGCTGTCGCGCCT
TGCCGGCTGCATTGCCGAGT

Product: rhodanese-like protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSDFSGLALVIEPSDLLPRLESRDLILVDLTSAARYAEGHIPGARFVDPKRTQLGQAPAPGLLPSKEKLEELFGELGHRK
DAVYVVYDDEGGGWAGRFIWLLDVIGHDQYHYLNGGLPAWLAEGSPMSIQIPPAVGGPVALTLHEEPTATREYLQSRLGA
ADLAIWDARGPLEYSGEKVLAAKGGHIPGAVNFEWTAGMDQARQLRIRTDMPQILEDLGITRDKEIITHCQTHHRSGFTY
LVAKALGYPRVKGYAGSWGEWGNHPDTPVEL

Sequences:

>Translated_271_residues
MSDFSGLALVIEPSDLLPRLESRDLILVDLTSAARYAEGHIPGARFVDPKRTQLGQAPAPGLLPSKEKLEELFGELGHRK
DAVYVVYDDEGGGWAGRFIWLLDVIGHDQYHYLNGGLPAWLAEGSPMSIQIPPAVGGPVALTLHEEPTATREYLQSRLGA
ADLAIWDARGPLEYSGEKVLAAKGGHIPGAVNFEWTAGMDQARQLRIRTDMPQILEDLGITRDKEIITHCQTHHRSGFTY
LVAKALGYPRVKGYAGSWGEWGNHPDTPVEL
>Mature_270_residues
SDFSGLALVIEPSDLLPRLESRDLILVDLTSAARYAEGHIPGARFVDPKRTQLGQAPAPGLLPSKEKLEELFGELGHRKD
AVYVVYDDEGGGWAGRFIWLLDVIGHDQYHYLNGGLPAWLAEGSPMSIQIPPAVGGPVALTLHEEPTATREYLQSRLGAA
DLAIWDARGPLEYSGEKVLAAKGGHIPGAVNFEWTAGMDQARQLRIRTDMPQILEDLGITRDKEIITHCQTHHRSGFTYL
VAKALGYPRVKGYAGSWGEWGNHPDTPVEL

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=232, Percent_Identity=28.0172413793103, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI61835204, Length=234, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI194473681, Length=234, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI194473668, Length=234, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI87082121, Length=255, Percent_Identity=32.156862745098, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87081967, Length=252, Percent_Identity=25.3968253968254, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17561888, Length=249, Percent_Identity=24.4979919678715, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29707; Mature: 29576

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFSGLALVIEPSDLLPRLESRDLILVDLTSAARYAEGHIPGARFVDPKRTQLGQAPAP
CCCCCCEEEEEECHHHCCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCHHHHCCCCCCC
GLLPSKEKLEELFGELGHRKDAVYVVYDDEGGGWAGRFIWLLDVIGHDQYHYLNGGLPAW
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCEE
LAEGSPMSIQIPPAVGGPVALTLHEEPTATREYLQSRLGAADLAIWDARGPLEYSGEKVL
EECCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEE
AAKGGHIPGAVNFEWTAGMDQARQLRIRTDMPQILEDLGITRDKEIITHCQTHHRSGFTY
EECCCCCCCCEEEEECCCCCHHHEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
LVAKALGYPRVKGYAGSWGEWGNHPDTPVEL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDFSGLALVIEPSDLLPRLESRDLILVDLTSAARYAEGHIPGARFVDPKRTQLGQAPAP
CCCCCEEEEEECHHHCCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCHHHHCCCCCCC
GLLPSKEKLEELFGELGHRKDAVYVVYDDEGGGWAGRFIWLLDVIGHDQYHYLNGGLPAW
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCEE
LAEGSPMSIQIPPAVGGPVALTLHEEPTATREYLQSRLGAADLAIWDARGPLEYSGEKVL
EECCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEE
AAKGGHIPGAVNFEWTAGMDQARQLRIRTDMPQILEDLGITRDKEIITHCQTHHRSGFTY
EECCCCCCCCEEEEECCCCCHHHEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
LVAKALGYPRVKGYAGSWGEWGNHPDTPVEL
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]