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The map label for this gene is glnA [H]

Identifier: 77456572

GI number: 77456572

Start: 388163

End: 389569

Strand: Reverse

Name: glnA [H]

Synonym: Pfl01_0344

Alternate gene names: 77456572

Gene position: 389569-388163 (Counterclockwise)

Preceding gene: 77456576

Following gene: 77456571

Centisome position: 6.05

GC content: 58.21

Gene sequence:

>1407_bases
ATGTCGAAGTCGGTTCAACTCATCAAAGATCATGACGTCAAGTGGATTGATCTGCGCTTCACGGACACCAAAGGCACTCA
GCACCACGTGACCATGCCGGCTCGCGATGCGCTGGAAGACGACTTCTTCGAAGTTGGCAAGATGTTCGACGGTTCCTCCA
TCGCTGGCTGGAAAGGCATCGAAGCCTCCGACATGATCCTGCTGCCGGACGACGAAACCGCCGTTCTGGACCCGTTCACC
GAAGAGCCGACCCTGATCCTGGTCTGCGACATCATCGAACCTTCGACCATGCAAGGTTACGATCGCGACCCGCGCGCCAT
CGCCCGCCGCGCCGAGGAATACCTGAAGACCACCGGTATCGGTGACACCGTATTCGCCGGTCCAGAGCCTGAGTTCTTCA
TCTTCGACGAAGTGAAGTTCAAGTCCGACATCTCCGGTTCGATGTTCAAGATCTACTCCGAGCAAGGCTCGTGGATGTCC
GACCAGGACGTGGAAGGCGGCAACAAAGGCCACCGTCCAGGCGTGAAAGGTGGCTACTTCCCGGTTCCGCCGTTCGACCA
CGACCACGAAATCCGTACCTCCATGTGCAACGCACTGGAAGAAATGGGCCTGACCGTTGAAGTTCACCACCACGAAGTGG
CAACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAAGCCGACGAAACCCAGACCCTGAAATAC
GTTGTCCACAACGTTGCCGACGCCTACGGCCGTACCGCGACCTTCATGCCGAAGCCTCTGTACGGCGACAACGGTTCGGG
CATGCACGTTCACATGTCCATCTCCAAAGATGGCAAGAACACCTTCGCGGGTGAAGGCTATGCCGGCCTGTCCGACACCG
CCCTGTACTTCATCGGCGGCATCATCAAGCACGGTAAGGCCCTGAACGGCTTCACCAACCCGGCCACCAACTCCTACAAG
CGTCTGGTGCCAGGCTTCGAAGCTCCAGTGATGCTGGCCTACTCGGCTCGCAACCGTTCCGCCTCGATCCGTATTCCTTA
CGTCAACAGCCCGAAAGGCCGTCGTATCGAAGCCCGCTTCCCGGATCCGGCTGCCAACCCGTACCTGGCTTTCGCAGCGC
TGCTGATGGCTGGTCTGGACGGTATCCAGAACAAGATCCACCCAGGCGATGCTGCCGACAAGAACCTGTACGACCTGCCG
CCTGAAGAGGCGAAAGAGATCCCGCAAGTTTGCGGCAGCCTGAAAGAAGCCCTGGAAGAGCTGGACAAGGGCCGTGCGTT
CCTGACCAAGGGCGGCGTGTTCTCCGACGACTTCATCGACGCTTACATCGCGCTGAAATCCGAAGAAGAAATCAAGGTCC
GCACCTTCGTACACCCACTGGAATATGAGCTGTACTACAGCTGCTGA

Upstream 100 bases:

>100_bases
TTGCTCCCTTGTGAGGCAGGTTGCCTTGGCAGAGTATTCGCGCCGGCATCACCACTATTAAGGGCATCCACTACCCGCCC
TAATTCACCCGGAGGACACT

Downstream 100 bases:

>100_bases
TCCGGTAGCGCCGCGCTACGCGGCGTGACAAAGAAAGGCCTCCTTCGGGAGGCCTTTTTTGTGCCCGCTCATCCATGGAT
AATCCGCCGCTTTCGCGCCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGIEASDMILLPDDETAVLDPFT
EEPTLILVCDIIEPSTMQGYDRDPRAIARRAEEYLKTTGIGDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMS
DQDVEGGNKGHRPGVKGGYFPVPPFDHDHEIRTSMCNALEEMGLTVEVHHHEVATAGQNEIGVKFNTLVKKADETQTLKY
VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGYAGLSDTALYFIGGIIKHGKALNGFTNPATNSYK
RLVPGFEAPVMLAYSARNRSASIRIPYVNSPKGRRIEARFPDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLP
PEEAKEIPQVCGSLKEALEELDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC

Sequences:

>Translated_468_residues
MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGIEASDMILLPDDETAVLDPFT
EEPTLILVCDIIEPSTMQGYDRDPRAIARRAEEYLKTTGIGDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMS
DQDVEGGNKGHRPGVKGGYFPVPPFDHDHEIRTSMCNALEEMGLTVEVHHHEVATAGQNEIGVKFNTLVKKADETQTLKY
VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGYAGLSDTALYFIGGIIKHGKALNGFTNPATNSYK
RLVPGFEAPVMLAYSARNRSASIRIPYVNSPKGRRIEARFPDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLP
PEEAKEIPQVCGSLKEALEELDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC
>Mature_467_residues
SKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGIEASDMILLPDDETAVLDPFTE
EPTLILVCDIIEPSTMQGYDRDPRAIARRAEEYLKTTGIGDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSD
QDVEGGNKGHRPGVKGGYFPVPPFDHDHEIRTSMCNALEEMGLTVEVHHHEVATAGQNEIGVKFNTLVKKADETQTLKYV
VHNVADAYGRTATFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGYAGLSDTALYFIGGIIKHGKALNGFTNPATNSYKR
LVPGFEAPVMLAYSARNRSASIRIPYVNSPKGRRIEARFPDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPP
EEAKEIPQVCGSLKEALEELDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=398, Percent_Identity=26.1306532663317, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=69.3965517241379, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=256, Percent_Identity=28.515625, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51803; Mature: 51672

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGI
CCCCCCEEECCCEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
EASDMILLPDDETAVLDPFTEEPTLILVCDIIEPSTMQGYDRDPRAIARRAEEYLKTTGI
CCCCEEEECCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDVEGGNKGHRPGVKGGYF
CCCEECCCCCCEEEEECEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPPFDHDHEIRTSMCNALEEMGLTVEVHHHEVATAGQNEIGVKFNTLVKKADETQTLKY
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEHHCCCCCCCEEHHHHHHHCCHHHHHHH
VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGYAGLSDTALYFIGG
HHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IIKHGKALNGFTNPATNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVNSPKGRRIEARF
HHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEC
PDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC
HHCCCEEEECCCCCCHHHHEEEEEECCCCCEEEEEEECCEEEEEEECC
>Mature Secondary Structure 
SKSVQLIKDHDVKWIDLRFTDTKGTQHHVTMPARDALEDDFFEVGKMFDGSSIAGWKGI
CCCCCEEECCCEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
EASDMILLPDDETAVLDPFTEEPTLILVCDIIEPSTMQGYDRDPRAIARRAEEYLKTTGI
CCCCEEEECCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDTVFAGPEPEFFIFDEVKFKSDISGSMFKIYSEQGSWMSDQDVEGGNKGHRPGVKGGYF
CCCEECCCCCCEEEEECEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPPFDHDHEIRTSMCNALEEMGLTVEVHHHEVATAGQNEIGVKFNTLVKKADETQTLKY
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEHHCCCCCCCEEHHHHHHHCCHHHHHHH
VVHNVADAYGRTATFMPKPLYGDNGSGMHVHMSISKDGKNTFAGEGYAGLSDTALYFIGG
HHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
IIKHGKALNGFTNPATNSYKRLVPGFEAPVMLAYSARNRSASIRIPYVNSPKGRRIEARF
HHHCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEC
PDPAANPYLAFAALLMAGLDGIQNKIHPGDAADKNLYDLPPEEAKEIPQVCGSLKEALEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDKGRAFLTKGGVFSDDFIDAYIALKSEEEIKVRTFVHPLEYELYYSC
HHCCCEEEECCCCCCHHHHEEEEEECCCCCEEEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]