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The map label for this gene is ntrB [H]

Identifier: 77456568

GI number: 77456568

Start: 384200

End: 385285

Strand: Reverse

Name: ntrB [H]

Synonym: Pfl01_0340

Alternate gene names: 77456568

Gene position: 385285-384200 (Counterclockwise)

Preceding gene: 77456569

Following gene: 77456567

Centisome position: 5.98

GC content: 63.08

Gene sequence:

>1086_bases
ATGACCATCAGTGACGCACTGCATCGCTTGCTACTCGACAACCTGACCACCGCCACCATCCTGCTCGACAGCGAATTGCG
CCTCGAGTACATGAACCCGGCGGCGGAGATGCTGCTGGCCATCAGCGGTCAGCGCAGCCATGGGCAGTTCATCAGCGAGC
TGTTCACCGAGTCCACCGAGGCGCTCAACTCGCTGCGCCAGGCGGTGGAGCAGGCGCACCCGTTCACCAAGCGCGAAGCG
ATGCTCACCGCCCTCACCGGCCAGACCCTGACCGTGGATTACGCGGTGACGCCGATCCTCAGCAACGGCGCGACCCTGCT
CCTGCTGGAAGTCCATCCCCGTGACCGCCTGCTGCGGATCACCAAGGAAGAGGCGCAACTGTCGAAACAGGAAACCAGCA
AGATGCTGGTGCGCGGCCTGGCCCACGAGATCAAGAATCCGCTGGGCGGCATCCGTGGTGCCGCGCAGTTGCTGGCCCGG
GAACTGCCGGAAGACAGCCTGCGCGACTACACCAACGTGATCATCGAAGAGGCCGACCGCCTGCGCAATCTGGTCGACCG
CATGCTCGGCTCGAACAAATTGCCGTCGCTGGCCCTGTGCAACGTACACGAAGTGCTGGAGCGGGTCTGCCAACTGGTCG
AGGCCGAAAGCCAGGGCTGCATCACGCTGGTGCGCGATTACGACCCGAGCATTCCAGACGTCCTGATCGACCGGGAACAA
ATGATTCAGGCGGTGCTGAACATCGTGCGCAACGCGATGCAGGCCATCAGCAGCCAGAACGAACTGCGCCTGGGCCGCAT
CAGCCTGCGCACCCGCGCCATGCGCCAGTTCACCATCGGCCACGTCCGCCATCGCCTGGTGACCAAGATCGAGATCATCG
ACAACGGCCCGGGCATTCCTGCGGAACTCCAGGAAACCATCTTCTTTCCCATGGTCAGCGGCCGTCCGGACGGTACCGGG
CTGGGCCTGGCCATCACCCAGAACATCATCAGCCAGCACCAGGGCCTGATCGAGTGTGACAGCCATCCAGGCCACACCAC
GTTCTCGATCTTTCTGCCACTGGAACAAGGAGCCACATCGACATGA

Upstream 100 bases:

>100_bases
AGGGGTTGAACGCTTCTTTTCGGAGCCTTGGTTTGGTTTTTGCATTTTCCTCGCATCAGCGCTGTATCCCTGCGCGCGCC
ATGCTCCAAAAGAGGTCCTC

Downstream 100 bases:

>100_bases
GCCGTAGTGAAACCGTGTGGATCGTCGATGACGACCGTTCTATCCGTTGGGTTCTGGAAAAAGCCCTGCAACAGGAAGGC
ATGACCACCCAGAGCTTCGA

Product: Signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTISDALHRLLLDNLTTATILLDSELRLEYMNPAAEMLLAISGQRSHGQFISELFTESTEALNSLRQAVEQAHPFTKREA
MLTALTGQTLTVDYAVTPILSNGATLLLLEVHPRDRLLRITKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLAR
ELPEDSLRDYTNVIIEEADRLRNLVDRMLGSNKLPSLALCNVHEVLERVCQLVEAESQGCITLVRDYDPSIPDVLIDREQ
MIQAVLNIVRNAMQAISSQNELRLGRISLRTRAMRQFTIGHVRHRLVTKIEIIDNGPGIPAELQETIFFPMVSGRPDGTG
LGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATST

Sequences:

>Translated_361_residues
MTISDALHRLLLDNLTTATILLDSELRLEYMNPAAEMLLAISGQRSHGQFISELFTESTEALNSLRQAVEQAHPFTKREA
MLTALTGQTLTVDYAVTPILSNGATLLLLEVHPRDRLLRITKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLAR
ELPEDSLRDYTNVIIEEADRLRNLVDRMLGSNKLPSLALCNVHEVLERVCQLVEAESQGCITLVRDYDPSIPDVLIDREQ
MIQAVLNIVRNAMQAISSQNELRLGRISLRTRAMRQFTIGHVRHRLVTKIEIIDNGPGIPAELQETIFFPMVSGRPDGTG
LGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATST
>Mature_360_residues
TISDALHRLLLDNLTTATILLDSELRLEYMNPAAEMLLAISGQRSHGQFISELFTESTEALNSLRQAVEQAHPFTKREAM
LTALTGQTLTVDYAVTPILSNGATLLLLEVHPRDRLLRITKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLARE
LPEDSLRDYTNVIIEEADRLRNLVDRMLGSNKLPSLALCNVHEVLERVCQLVEAESQGCITLVRDYDPSIPDVLIDREQM
IQAVLNIVRNAMQAISSQNELRLGRISLRTRAMRQFTIGHVRHRLVTKIEIIDNGPGIPAELQETIFFPMVSGRPDGTGL
GLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATST

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=46.3068181818182, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1788549, Length=369, Percent_Identity=29.810298102981, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=33.0357142857143, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081816, Length=250, Percent_Identity=30, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=274, Percent_Identity=28.1021897810219, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=253, Percent_Identity=26.4822134387352, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=26.8085106382979, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40070; Mature: 39939

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISDALHRLLLDNLTTATILLDSELRLEYMNPAAEMLLAISGQRSHGQFISELFTESTE
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALNSLRQAVEQAHPFTKREAMLTALTGQTLTVDYAVTPILSNGATLLLLEVHPRDRLLRI
HHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECHHCCCCCEEEEEEECCCHHHHHH
TKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLARELPEDSLRDYTNVIIEEADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LRNLVDRMLGSNKLPSLALCNVHEVLERVCQLVEAESQGCITLVRDYDPSIPDVLIDREQ
HHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCHHH
MIQAVLNIVRNAMQAISSQNELRLGRISLRTRAMRQFTIGHVRHRLVTKIEIIDNGPGIP
HHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
AELQETIFFPMVSGRPDGTGLGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATS
HHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCCCC
T
C
>Mature Secondary Structure 
TISDALHRLLLDNLTTATILLDSELRLEYMNPAAEMLLAISGQRSHGQFISELFTESTE
CHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALNSLRQAVEQAHPFTKREAMLTALTGQTLTVDYAVTPILSNGATLLLLEVHPRDRLLRI
HHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEEECHHCCCCCEEEEEEECCCHHHHHH
TKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLARELPEDSLRDYTNVIIEEADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LRNLVDRMLGSNKLPSLALCNVHEVLERVCQLVEAESQGCITLVRDYDPSIPDVLIDREQ
HHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHCCHHH
MIQAVLNIVRNAMQAISSQNELRLGRISLRTRAMRQFTIGHVRHRLVTKIEIIDNGPGIP
HHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
AELQETIFFPMVSGRPDGTGLGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATS
HHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEECCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]