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The map label for this gene is glnG [H]

Identifier: 77456567

GI number: 77456567

Start: 382767

End: 384203

Strand: Reverse

Name: glnG [H]

Synonym: Pfl01_0339

Alternate gene names: 77456567

Gene position: 384203-382767 (Counterclockwise)

Preceding gene: 77456568

Following gene: 77456565

Centisome position: 5.97

GC content: 64.44

Gene sequence:

>1437_bases
ATGAGCCGTAGTGAAACCGTGTGGATCGTCGATGACGACCGTTCTATCCGTTGGGTTCTGGAAAAAGCCCTGCAACAGGA
AGGCATGACCACCCAGAGCTTCGACAGTGCCGATGGCGTGATGAGCCGCCTCGCGCGCCAGCAGCCGGACGTGATCATCT
CCGACATCCGCATGCCCGGCGCCAGTGGCCTGGACCTGCTGGCACGGATCCGCGAGCAGCACCCGCGGCTGCCGGTGATC
ATCATGACCGCTCATTCCGATCTGGACAGCGCTGTCGCCTCCTATCAGGGTGGCGCGTTCGAATACCTGCCCAAGCCGTT
CGACGTCGACGAAGCCGTGTCGCTGGTCAAGCGCGCCAATCAGCACGCCCAGGAACAGCAAGGTCTGGAAGTCGCGCCAG
CGCTGACCCGCACCCCGGAAATCATCGGCGAAGCGCCGGCGATGCAGGAAGTGTTTCGCGCCATCGGGCGCCTGAGCCAC
TCCAACATCACCGTGCTGATCAACGGCGAATCCGGCACCGGTAAAGAACTGGTGGCCCACGCCCTGCACCGCCACAGCCC
GCGCGCGGCCTCGCCGTTCATCGCGCTGAACATGGCGGCGATTCCGAAAGACCTGATGGAATCCGAGCTGTTCGGCCATG
AAAAAGGCGCGTTCACCGGTGCGGCCAACCTGCGTCGTGGCCGTTTCGAACAGGCCGACGGCGGCACGCTGTTCCTCGAT
GAAATCGGCGACATGCCGGCGGACACCCAGACTCGCCTGCTGCGGGTGCTGGCCGATGGCGAGTTCTATCGCGTTGGCGG
GCACGTGCCGGTCAAAGTCGATGTGCGGATCATCGCCGCAACGCACCAGAATCTGGAAACCCTGGTTCACGCCGGGAAAT
TCCGTGAGGACTTGTTCCACCGCCTCAACGTGATCCGCATCCACATCCCTCGCCTGTCGGACCGTCGCGAAGACATCCCG
ACCCTGGCCAAGCACTTCCTCAGCCGCGCCGCGCAAGAGCTGGCGGTGGAGCCGAAGCTGCTGAAAAGCGAGACCGAGGA
ATACCTGAAGAACCTGCCGTGGCCAGGCAACGTGCGGCAGCTGGAAAACACCTGCCGCTGGATCACCGTAATGGCCTCCG
GGCGCGAAGTGCACATCAGCGACCTGCCGCCGGAACTGCTGAGCCTGCCGCAGGACTCGGCGCCGGTCACCAACTGGGAA
CAGGCGCTGCGACAGTGGGCCGATCAGGCCCTGGCGCGCGGTCAGTCGAGCCTGCTCGACAGCGCGGTGCCGGCGTTCGA
GCGAATCATGATCGAGACAGCACTGAAGCACACCGCCGGACGTCGTCGCGACGCGGCCGTGCTACTGGGCTGGGGCCGGA
ACACCTTGACCCGCAAGATCAAGGAACTCGGCATGAAGGTCGATGGCGGGGATGACGATGAGGGCGATGAAGGCTGA

Upstream 100 bases:

>100_bases
ACATCATCAGCCAGCACCAGGGCCTGATCGAGTGTGACAGCCATCCAGGCCACACCACGTTCTCGATCTTTCTGCCACTG
GAACAAGGAGCCACATCGAC

Downstream 100 bases:

>100_bases
AATTCAGCGCCAGCCTGCACCGCCGCAAGGCACCGTGACCCTTAATCGGGCACGGCTACTGGCTCAGGCTTGCACTGTAA
ACCGCTAAAAATCCCATCCT

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLEVAPALTRTPEIIGEAPAMQEVFRAIGRLSH
SNITVLINGESGTGKELVAHALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
EIGDMPADTQTRLLRVLADGEFYRVGGHVPVKVDVRIIAATHQNLETLVHAGKFREDLFHRLNVIRIHIPRLSDRREDIP
TLAKHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWE
QALRQWADQALARGQSSLLDSAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG

Sequences:

>Translated_478_residues
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVI
IMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLEVAPALTRTPEIIGEAPAMQEVFRAIGRLSH
SNITVLINGESGTGKELVAHALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
EIGDMPADTQTRLLRVLADGEFYRVGGHVPVKVDVRIIAATHQNLETLVHAGKFREDLFHRLNVIRIHIPRLSDRREDIP
TLAKHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWE
QALRQWADQALARGQSSLLDSAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG
>Mature_477_residues
SRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPGASGLDLLARIREQHPRLPVII
MTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRANQHAQEQQGLEVAPALTRTPEIIGEAPAMQEVFRAIGRLSHS
NITVLINGESGTGKELVAHALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLDE
IGDMPADTQTRLLRVLADGEFYRVGGHVPVKVDVRIIAATHQNLETLVHAGKFREDLFHRLNVIRIHIPRLSDRREDIPT
LAKHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQLENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWEQ
ALRQWADQALARGQSSLLDSAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=68.75, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=40.9381663113006, Blast_Score=348, Evalue=6e-97,
Organism=Escherichia coli, GI1790437, Length=455, Percent_Identity=40.2197802197802, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=42.4324324324324, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI87082117, Length=336, Percent_Identity=43.1547619047619, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=42.3880597014925, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=42.5531914893617, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=340, Percent_Identity=40, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1786524, Length=339, Percent_Identity=41.2979351032448, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=38.6503067484663, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=46.2555066079295, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI87081858, Length=408, Percent_Identity=28.4313725490196, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=36.1607142857143, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1790860, Length=113, Percent_Identity=36.283185840708, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789809, Length=191, Percent_Identity=26.1780104712042, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786911, Length=106, Percent_Identity=33.0188679245283, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790552, Length=124, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786599, Length=150, Percent_Identity=29.3333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53042; Mature: 52911

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPG
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
ASGLDLLARIREQHPRLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRAN
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
QHAQEQQGLEVAPALTRTPEIIGEAPAMQEVFRAIGRLSHSNITVLINGESGTGKELVAH
HHHHHHCCCEECCHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPADTQTRLLRVLADGEFYRVGGHVPVKVDVRIIAATHQNLETLVHAGKFREDLFH
HHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
RLNVIRIHIPRLSDRREDIPTLAKHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQ
HCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
LENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWEQALRQWADQALARGQSSLLD
HHHHCEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
SAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPG
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCC
ASGLDLLARIREQHPRLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVSLVKRAN
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHH
QHAQEQQGLEVAPALTRTPEIIGEAPAMQEVFRAIGRLSHSNITVLINGESGTGKELVAH
HHHHHHCCCEECCHHHCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
ALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
HHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHH
EIGDMPADTQTRLLRVLADGEFYRVGGHVPVKVDVRIIAATHQNLETLVHAGKFREDLFH
HHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
RLNVIRIHIPRLSDRREDIPTLAKHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQ
HCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
LENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWEQALRQWADQALARGQSSLLD
HHHHCEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
SAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]