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The map label for this gene is hisH [H]

Identifier: 77456552

GI number: 77456552

Start: 370155

End: 370793

Strand: Direct

Name: hisH [H]

Synonym: Pfl01_0324

Alternate gene names: 77456552

Gene position: 370155-370793 (Clockwise)

Preceding gene: 77456551

Following gene: 77456553

Centisome position: 5.75

GC content: 62.75

Gene sequence:

>639_bases
ATGCAGACGGTTGCAGTTATCGACTACGGCATGGGCAACCTGCACTCGGTGGCCAAGGCCCTCGAGCACGTTGGTGCCGG
CAAGGTGCTGATCACCAGCGATGCGGCAGTGATCCGCGAAGCCGACCGCGTGGTGTTCCCCGGTGTCGGCGCGATTCGCG
ATTGCATGGCGGAGATCCGTCGCCTCGGTTTCGATTCGCTGGTGCGTGAAGTCAGCCAGGATCGTCCGTTCCTCGGCATT
TGCGTCGGCATGCAAGCCTTGCTCGACAGCAGCGAAGAGAACGACGGCGTCGACTGCATCGGCCTGTTCCCGGGCGCGGT
GAAGTTCTTCGGCAAAGACCTGCATGAAGACGGCGAGCACCTGAAAGTCCCGCACATGGGCTGGAACGAAGTGAAGCAGG
CGGTGGATCACCCGCTGTGGCACAACATTCCGGACCTGGCGCGTTTCTACTTCGTGCACAGCTACTACATCGCCGCCGGT
AACCCGCGGCAGGTGGTCGGCAGCGGTCATTACGGCGTCGATTTCGCCGCGGCGCTGGCCGAAGGTTCGCGTTTCGCCGT
GCAGTTCCACCCGGAGAAGAGCCATACCCATGGCCTGCAATTGCTGCAGAACTTCGCGGCGTGGGACGGTCGCTGGTAA

Upstream 100 bases:

>100_bases
TCGAAACCGTGTTCAAGGCTTTCGGCCGCGCGCTGCGCATGGCCGTCGAGCTGGACGAGCGCATGGCCGGCCAGATGCCT
TCGACCAAGGGCGTCCTGTA

Downstream 100 bases:

>100_bases
ATGGCTGTCAAGAAATCCAAACCGCCGATCCTGACCCTCACTCCCGAGCAGGAGAGCGAGGCCAACCGCAAGATTCAGCG
GTTCATGGAGGATCGTTTCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1 [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLITSDAAVIREADRVVFPGVGAIRDCMAEIRRLGFDSLVREVSQDRPFLGI
CVGMQALLDSSEENDGVDCIGLFPGAVKFFGKDLHEDGEHLKVPHMGWNEVKQAVDHPLWHNIPDLARFYFVHSYYIAAG
NPRQVVGSGHYGVDFAAALAEGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW

Sequences:

>Translated_212_residues
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLITSDAAVIREADRVVFPGVGAIRDCMAEIRRLGFDSLVREVSQDRPFLGI
CVGMQALLDSSEENDGVDCIGLFPGAVKFFGKDLHEDGEHLKVPHMGWNEVKQAVDHPLWHNIPDLARFYFVHSYYIAAG
NPRQVVGSGHYGVDFAAALAEGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW
>Mature_212_residues
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLITSDAAVIREADRVVFPGVGAIRDCMAEIRRLGFDSLVREVSQDRPFLGI
CVGMQALLDSSEENDGVDCIGLFPGAVKFFGKDLHEDGEHLKVPHMGWNEVKQAVDHPLWHNIPDLARFYFVHSYYIAAG
NPRQVVGSGHYGVDFAAALAEGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=37.1980676328502, Blast_Score=124, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=31.4814814814815, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23321; Mature: 23321

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLITSDAAVIREADRVVFPGVGAIRDCMAEIR
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEECCCCHHHHHHHHHHH
RLGFDSLVREVSQDRPFLGICVGMQALLDSSEENDGVDCIGLFPGAVKFFGKDLHEDGEH
HCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCE
LKVPHMGWNEVKQAVDHPLWHNIPDLARFYFVHSYYIAAGNPRQVVGSGHYGVDFAAALA
EECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHH
EGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQTVAVIDYGMGNLHSVAKALEHVGAGKVLITSDAAVIREADRVVFPGVGAIRDCMAEIR
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEECCCCHHHHHHHHHHH
RLGFDSLVREVSQDRPFLGICVGMQALLDSSEENDGVDCIGLFPGAVKFFGKDLHEDGEH
HCCHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCE
LKVPHMGWNEVKQAVDHPLWHNIPDLARFYFVHSYYIAAGNPRQVVGSGHYGVDFAAALA
EECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHH
EGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]