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The map label for this gene is artJ [H]

Identifier: 77456539

GI number: 77456539

Start: 357019

End: 357765

Strand: Reverse

Name: artJ [H]

Synonym: Pfl01_0311

Alternate gene names: 77456539

Gene position: 357765-357019 (Counterclockwise)

Preceding gene: 77456540

Following gene: 77456538

Centisome position: 5.56

GC content: 57.3

Gene sequence:

>747_bases
ATGCAGAACTACAAAAAGGTCTTCCTGGCCGCCGCCGTCACCCTCGCGTTCAGCGCCGGTGCCATGGCCGAGACCCTGAA
GATGGGCATCGAAGCGGCTTACCCGCCGTTCAACAATAAAGACGCCAGCGGCAACGTCGTCGGCTTCGACAAAGACATCG
GCGACGCCCTGTGCGCCAAGATGAAAGTTGAATGCTCCGTGGTCACCTCCGACTGGGACGGCATCATCCCGGCCCTGAAC
GCCAAGAAGTTCGACTTTCTGATCTCCTCGATGTCGATCACCGACGAGCGCAAGGGCGCGGTGGACTTCACCGACCCGTA
CTACTCCAACAAACTGCAATTCATCGCGCCGAAGGATAAAGAGTTCAAGACCGACAAGGACTCCCTCAAAGGCAAAGTGA
TCGGCGCCCAGCGCGCGACCCTCGCCGGTACCTTCATGGAAGACAACATGCCGGGCGTCGAAGTAAAACTCTACGACACC
CAGGAAAACGCCTACCTCGACCTGATCTCCGGTCGCCTGGACGGCATCCTCGCCGACAAATACGTCAACTACGAGTGGCT
GAAAAGCGACGCCGGCAAAGCCTATGAATTCAAGGGCGATCCGGTGGAAGAAAGCGACAAGATCGGTATCGCTGTACGCA
AAGGCGACCCGATCCGCGAGAAGCTGAATGCTGCGCTGAAGGAAATCGTTGCCGACGGCACCTACAAGAAGATCAACGAC
AAGTACTTCCCGTTCAGCATCTACTGA

Upstream 100 bases:

>100_bases
CTGGTAGAAGAGCAAGGATCGCCACAGCAGGTGTTCGAAAACCCGCTTTCGGCGCGCTGCAAACAATTCATGTCCAGCAA
CCGCTAACGGAGCTACCCGC

Downstream 100 bases:

>100_bases
TACTGACCTGCCGGATCGGCGCCGCCAAATGACGGCGCCGATCCCCGGCCTTGCCTGCCGCGATTTGAAAAGAAATCCAT
GATTATCGACCTCTACGGAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQNYKKVFLAAAVTLAFSAGAMAETLKMGIEAAYPPFNNKDASGNVVGFDKDIGDALCAKMKVECSVVTSDWDGIIPALN
AKKFDFLISSMSITDERKGAVDFTDPYYSNKLQFIAPKDKEFKTDKDSLKGKVIGAQRATLAGTFMEDNMPGVEVKLYDT
QENAYLDLISGRLDGILADKYVNYEWLKSDAGKAYEFKGDPVEESDKIGIAVRKGDPIREKLNAALKEIVADGTYKKIND
KYFPFSIY

Sequences:

>Translated_248_residues
MQNYKKVFLAAAVTLAFSAGAMAETLKMGIEAAYPPFNNKDASGNVVGFDKDIGDALCAKMKVECSVVTSDWDGIIPALN
AKKFDFLISSMSITDERKGAVDFTDPYYSNKLQFIAPKDKEFKTDKDSLKGKVIGAQRATLAGTFMEDNMPGVEVKLYDT
QENAYLDLISGRLDGILADKYVNYEWLKSDAGKAYEFKGDPVEESDKIGIAVRKGDPIREKLNAALKEIVADGTYKKIND
KYFPFSIY
>Mature_248_residues
MQNYKKVFLAAAVTLAFSAGAMAETLKMGIEAAYPPFNNKDASGNVVGFDKDIGDALCAKMKVECSVVTSDWDGIIPALN
AKKFDFLISSMSITDERKGAVDFTDPYYSNKLQFIAPKDKEFKTDKDSLKGKVIGAQRATLAGTFMEDNMPGVEVKLYDT
QENAYLDLISGRLDGILADKYVNYEWLKSDAGKAYEFKGDPVEESDKIGIAVRKGDPIREKLNAALKEIVADGTYKKIND
KYFPFSIY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=44.2622950819672, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1788649, Length=249, Percent_Identity=39.7590361445783, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=40, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=36.1702127659575, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=32.8828828828829, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=34.412955465587, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27406; Mature: 27406

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNYKKVFLAAAVTLAFSAGAMAETLKMGIEAAYPPFNNKDASGNVVGFDKDIGDALCAK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHH
MKVECSVVTSDWDGIIPALNAKKFDFLISSMSITDERKGAVDFTDPYYSNKLQFIAPKDK
EEEEEEEEECCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
EFKTDKDSLKGKVIGAQRATLAGTFMEDNMPGVEVKLYDTQENAYLDLISGRLDGILADK
CCCCCHHHCCEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEHHCCCCCEEHHH
YVNYEWLKSDAGKAYEFKGDPVEESDKIGIAVRKGDPIREKLNAALKEIVADGTYKKIND
HCCHHEECCCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
KYFPFSIY
CCCCEEEC
>Mature Secondary Structure
MQNYKKVFLAAAVTLAFSAGAMAETLKMGIEAAYPPFNNKDASGNVVGFDKDIGDALCAK
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHH
MKVECSVVTSDWDGIIPALNAKKFDFLISSMSITDERKGAVDFTDPYYSNKLQFIAPKDK
EEEEEEEEECCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCC
EFKTDKDSLKGKVIGAQRATLAGTFMEDNMPGVEVKLYDTQENAYLDLISGRLDGILADK
CCCCCHHHCCEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCEEEEHHCCCCCEEHHH
YVNYEWLKSDAGKAYEFKGDPVEESDKIGIAVRKGDPIREKLNAALKEIVADGTYKKIND
HCCHHEECCCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCC
KYFPFSIY
CCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]