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The map label for this gene is fadJ [H]

Identifier: 77456504

GI number: 77456504

Start: 322487

End: 323710

Strand: Direct

Name: fadJ [H]

Synonym: Pfl01_0276

Alternate gene names: 77456504

Gene position: 322487-323710 (Clockwise)

Preceding gene: 77456503

Following gene: 77456505

Centisome position: 5.01

GC content: 62.34

Gene sequence:

>1224_bases
ATGAGCCAGACATCCTTCGCTATTCAGCAGGCCGCCGTGATCGGCGCCGGCACCATGGGCCGCGGCATTGTCATGTGCCT
GGCCAATGCCGGCGTGTCAGTGCAGTGGGTCGATAACAATCCACAGATGCTCGAACAGGCGCTGTCCAGCGTGGCCGAGA
CTTACGCACACAACGTGCGGCAGGGACGGATCGATCAGGCCGAAGCCGATGCGCGGATTGCCCGTGTCAGCAAGGCGGCC
GACTATGTGGCGATCCGCAATGTCGATCTGGTGATCGAAGCGGTGTACGAGAATCTTGAGCTCAAGCAGAAGATCTTCCG
CGAGCTCGATGGCGTGCTGAAGCCCGAGGCGCTGCTGGCGAGCAATACTTCGGCGCTGGACATCGACGCCATTGCCGCCG
CGACACGGCGCCCGACTCAAGTGTTGGGCCTGCATTTCTTCAGCCCGGCACACATCATGAAGTTGCTGGAAATAGTCCGT
GGCGCGCAGACCGCACCGGCTGTGCTGGAGGCTGCGCTTGAGCTGGGCAAGCGCATGGGCAAGGTCAGTGTGGTATCGGG
CAACTGCCACGGCTTTATCGGCAATCGCATGCTCCATCCGTACGTGCTCGAAGCGCGCAAGATGTTGCTCGAAGGGGCGT
ACCCGCATCAGGTCGATGCAGCCTTGCAGAGCTTCGGTTTTGCCATGGGGCCGTTCCGCATGTACGACGTGGTCGGCATC
GATCTCGAGTGGCGTGCCCGCGAGCTGGCAGGCAAGGGGCAGGACGCGCCGGAGGTTCAGGTGGATAACCGTTTGTGCGA
GCAGGGTCGGTTCGGTCAGAAGTCCGGCAACGGTTATTACCATTACGAGCCGGGCAGCCGTCAGGCCGAGCATGATCCCG
AGGTCGATGCGCTGGTGTTGAGTGTCAGCGAAGGGTTGGGATTTCATCGGCGGGATATCGGCTCGGAGGAGATCCTCGAG
CGGTGTCTGCTGGCGTTGGTCAACGAGGGTGCGAAGATCCTGCAGGAAGGGATTGCCGAGTCGGCGCATGACATCGATCA
GGTCTATCTGAATGGCTATGGCTTCCCGGCGGAGCGGGGCGGGCCGATGGCCTGGGCGGACGATCAGGGGCTGGCCGATA
TTCACAAGCGATTGCTGGCGCTGGAGACTCGGCAGGGCGATCACTGGCGGCCGGCGCCATTGATCGGCGAGCTGGCGGCG
CGAGGCAAGGGTTTTTACCTTTAG

Upstream 100 bases:

>100_bases
TCCATCTGCTGAATGTCGATCATCACGGCAATGAACTTCAAAAATGCCCGGCGGCTGACTAAGGTGATGGCTGTCTTCGG
ATCGCCAACAAGAGAACAAC

Downstream 100 bases:

>100_bases
ACTGAGCAGATCACTCCAGGAATAGACACTGATGTCCAACCCGATGCCGCAACGCAGCGATTACCCGCACTTCCAGCCGA
TCACCACGCGCTGGCACGAC

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSQTSFAIQQAAVIGAGTMGRGIVMCLANAGVSVQWVDNNPQMLEQALSSVAETYAHNVRQGRIDQAEADARIARVSKAA
DYVAIRNVDLVIEAVYENLELKQKIFRELDGVLKPEALLASNTSALDIDAIAAATRRPTQVLGLHFFSPAHIMKLLEIVR
GAQTAPAVLEAALELGKRMGKVSVVSGNCHGFIGNRMLHPYVLEARKMLLEGAYPHQVDAALQSFGFAMGPFRMYDVVGI
DLEWRARELAGKGQDAPEVQVDNRLCEQGRFGQKSGNGYYHYEPGSRQAEHDPEVDALVLSVSEGLGFHRRDIGSEEILE
RCLLALVNEGAKILQEGIAESAHDIDQVYLNGYGFPAERGGPMAWADDQGLADIHKRLLALETRQGDHWRPAPLIGELAA
RGKGFYL

Sequences:

>Translated_407_residues
MSQTSFAIQQAAVIGAGTMGRGIVMCLANAGVSVQWVDNNPQMLEQALSSVAETYAHNVRQGRIDQAEADARIARVSKAA
DYVAIRNVDLVIEAVYENLELKQKIFRELDGVLKPEALLASNTSALDIDAIAAATRRPTQVLGLHFFSPAHIMKLLEIVR
GAQTAPAVLEAALELGKRMGKVSVVSGNCHGFIGNRMLHPYVLEARKMLLEGAYPHQVDAALQSFGFAMGPFRMYDVVGI
DLEWRARELAGKGQDAPEVQVDNRLCEQGRFGQKSGNGYYHYEPGSRQAEHDPEVDALVLSVSEGLGFHRRDIGSEEILE
RCLLALVNEGAKILQEGIAESAHDIDQVYLNGYGFPAERGGPMAWADDQGLADIHKRLLALETRQGDHWRPAPLIGELAA
RGKGFYL
>Mature_406_residues
SQTSFAIQQAAVIGAGTMGRGIVMCLANAGVSVQWVDNNPQMLEQALSSVAETYAHNVRQGRIDQAEADARIARVSKAAD
YVAIRNVDLVIEAVYENLELKQKIFRELDGVLKPEALLASNTSALDIDAIAAATRRPTQVLGLHFFSPAHIMKLLEIVRG
AQTAPAVLEAALELGKRMGKVSVVSGNCHGFIGNRMLHPYVLEARKMLLEGAYPHQVDAALQSFGFAMGPFRMYDVVGID
LEWRARELAGKGQDAPEVQVDNRLCEQGRFGQKSGNGYYHYEPGSRQAEHDPEVDALVLSVSEGLGFHRRDIGSEEILER
CLLALVNEGAKILQEGIAESAHDIDQVYLNGYGFPAERGGPMAWADDQGLADIHKRLLALETRQGDHWRPAPLIGELAAR
GKGFYL

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI68989263, Length=416, Percent_Identity=40.8653846153846, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI261878539, Length=416, Percent_Identity=40.8653846153846, Blast_Score=315, Evalue=3e-86,
Organism=Homo sapiens, GI20127408, Length=407, Percent_Identity=31.6953316953317, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI296179429, Length=293, Percent_Identity=34.4709897610922, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI296179427, Length=310, Percent_Identity=32.9032258064516, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI115430219, Length=221, Percent_Identity=26.6968325791855, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=385, Percent_Identity=34.5454545454545, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790281, Length=374, Percent_Identity=35.0267379679144, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1787661, Length=355, Percent_Identity=34.6478873239437, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71985923, Length=380, Percent_Identity=30.5263157894737, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71985930, Length=386, Percent_Identity=29.2746113989637, Blast_Score=237, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17508951, Length=408, Percent_Identity=32.843137254902, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17508953, Length=408, Percent_Identity=32.843137254902, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17558304, Length=408, Percent_Identity=32.5980392156863, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25144276, Length=285, Percent_Identity=35.0877192982456, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17549919, Length=295, Percent_Identity=35.5932203389831, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17553560, Length=291, Percent_Identity=31.9587628865979, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17563036, Length=288, Percent_Identity=29.1666666666667, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24583077, Length=404, Percent_Identity=31.1881188118812, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24583079, Length=404, Percent_Identity=31.1881188118812, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI19921000, Length=404, Percent_Identity=31.1881188118812, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI45549573, Length=209, Percent_Identity=25.3588516746411, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129993, Length=209, Percent_Identity=25.8373205741627, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 44437; Mature: 44306

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00067 3HCDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSFAIQQAAVIGAGTMGRGIVMCLANAGVSVQWVDNNPQMLEQALSSVAETYAHNVR
CCCHHHHHHHHHHHCCCCCCCHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QGRIDQAEADARIARVSKAADYVAIRNVDLVIEAVYENLELKQKIFRELDGVLKPEALLA
CCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
SNTSALDIDAIAAATRRPTQVLGLHFFSPAHIMKLLEIVRGAQTAPAVLEAALELGKRMG
CCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
KVSVVSGNCHGFIGNRMLHPYVLEARKMLLEGAYPHQVDAALQSFGFAMGPFRMYDVVGI
CEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEC
DLEWRARELAGKGQDAPEVQVDNRLCEQGRFGQKSGNGYYHYEPGSRQAEHDPEVDALVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHEEE
SVSEGLGFHRRDIGSEEILERCLLALVNEGAKILQEGIAESAHDIDQVYLNGYGFPAERG
EHHHCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GPMAWADDQGLADIHKRLLALETRQGDHWRPAPLIGELAARGKGFYL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQTSFAIQQAAVIGAGTMGRGIVMCLANAGVSVQWVDNNPQMLEQALSSVAETYAHNVR
CCHHHHHHHHHHHCCCCCCCHHHEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
QGRIDQAEADARIARVSKAADYVAIRNVDLVIEAVYENLELKQKIFRELDGVLKPEALLA
CCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
SNTSALDIDAIAAATRRPTQVLGLHFFSPAHIMKLLEIVRGAQTAPAVLEAALELGKRMG
CCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
KVSVVSGNCHGFIGNRMLHPYVLEARKMLLEGAYPHQVDAALQSFGFAMGPFRMYDVVGI
CEEEEECCCCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEEC
DLEWRARELAGKGQDAPEVQVDNRLCEQGRFGQKSGNGYYHYEPGSRQAEHDPEVDALVL
CCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHEEE
SVSEGLGFHRRDIGSEEILERCLLALVNEGAKILQEGIAESAHDIDQVYLNGYGFPAERG
EHHHCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GPMAWADDQGLADIHKRLLALETRQGDHWRPAPLIGELAARGKGFYL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA