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The map label for this gene is pckA [H]

Identifier: 77456496

GI number: 77456496

Start: 314585

End: 316126

Strand: Direct

Name: pckA [H]

Synonym: Pfl01_0268

Alternate gene names: 77456496

Gene position: 314585-316126 (Clockwise)

Preceding gene: 77456495

Following gene: 77456502

Centisome position: 4.89

GC content: 61.28

Gene sequence:

>1542_bases
ATGACGCAAGCCAATAACGCCGTGTACACCGATCTGAGTGTTGATGATCTGGTAAAAGAAGCTCTGAACCGCGGTGAGGG
CGAGCTTGCCGATACCGGCGCTCTGGTTGTTCGCACCGGTCATCGCACCGGCCGTTCGCCAGTCGACCGTTTCATCGTTG
AAGAGCCTTCCACCCAGGCTGCCATCGCCTGGGGCCCGATCAACCGCAAGTTCCCGGCCGACAAGTTCGACGCGCTGTGG
GCTCGTGTTGAAGCATTCAACAACGCGCAAGAGCATTTCGTTTCCCACGTGCATGTAGGCGCGGCCGAAGATCACTACCT
GGCCGTGAAGATGACCACCCAGACTGCCTGGCAGAACCTGTTCGGTCGTTGCCTGTTCATCAACCCGGCCCAGTACAACC
CGGCCGGTCGCGAAGAGTGGCAAGTGCTGAACGTCGCCAACTTCGAGTGCGTGCCTGAGCGTGACGGCACCAACTCCGAT
GGTTGCGTGATCATCAACTTCGCCCAGAAGAAAGTGCTGATCGCCGGCATGCGTTACGCCGGTGAAATGAAGAAAGCCAT
GTTCTCCGTGCAGAACTTCCTGCTGCCGGCCGCTGACGTGCTGCCGATGCACTGCGCTGCCAACATCGGCGAAGAAGGCG
ACGTGACCCTGTTCTTCGGTCTGTCGGGCACCGGCAAGACCACCCTGTCCGCCGACGAAAGCCGTTACCTGATCGGTGAC
GACGAACACGGCTGGGGCGAAGGCGTTGTCTTCAACATCGAAGGCGGCTGCTACGCCAAGTGCATCGACTTGTCCGAGAA
GAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCGGTGCTGGAAAACGTCGTGATCGACGATGCCAAGCACGCCG
ACTACGCCGATGTCAGCCTGACCCAGAACAGCCGCGCGGCCTACCCGCTGGAGCACGTTGCCAAGCGTTCCGAGAAGAAC
CTGGGCGGCGAGCCGAACGCTGTGATCTTCCTGACCTGCGACCTGACCGGCGTGCTGCCGCCAGTGTCGATCCTCAACGA
AGAACAAGCGGCCTACCACTTCCTGTCCGGCTACACCGCACTGGTGGGCTCGACTGAAATGGGTTCGGGCAGCGGCATCA
AGTCGACCTTCTCCACCTGCTTCGGCGCACCGTTCTTCCCGCGTCCGGCCGGCGAATACGCTGAACTGCTGATCAAGCGC
ATCCGCGGCTTCGGCTCCAAGGTCTACCTGGTCAACACCGGCTGGACCGGCGGTGGCTACGGCGTCGGCAAACGCTTCAA
CATCCCGACCACCCGCGCGGTGATCGCAGCGATCCAGAGCGGCGCGCTGATCGGTGCAGCCACCGAGCACCTGGACACCA
TCAACCTGGACGTGCCACTGGCCGTACCGGGCGTCGAAACCAACCTGCTCAACCCACGCAACACCTGGGCTGACAAGGCT
GCGTACGACGAAGCTGCCAAAGCACTGGCCGGTCTGTTCATCGAGAACTTCAAGAAGTTCGAAGTGAGCGACGCGATCAA
GGCTGCCGGGCCTAAGTTGTAA

Upstream 100 bases:

>100_bases
TTCTGGCATAATCCGGCCCACTTTTTTCGCGGTAGTAGTGCACGACTTTCTACTACAAAACGTTTGGAGCACACTCGGCC
ACTGGCCGACGGGGAACCTC

Downstream 100 bases:

>100_bases
GACTTAGTTTTGGTGAATGAAAAAGCCGCCTGTTGAGGGCGGCTTTTTTGTGAGCGTTCATTTTCTCAATGGGCCAATGC
ATTCAAAACATGAACCGGGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQAAIAWGPINRKFPADKFDALW
ARVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNLFGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSD
GCVIINFAQKKVLIAGMRYAGEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSLTQNSRAAYPLEHVAKRSEKN
LGGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKR
IRGFGSKVYLVNTGWTGGGYGVGKRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVETNLLNPRNTWADKA
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPKL

Sequences:

>Translated_513_residues
MTQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQAAIAWGPINRKFPADKFDALW
ARVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNLFGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSD
GCVIINFAQKKVLIAGMRYAGEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSLTQNSRAAYPLEHVAKRSEKN
LGGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKR
IRGFGSKVYLVNTGWTGGGYGVGKRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVETNLLNPRNTWADKA
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPKL
>Mature_512_residues
TQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQAAIAWGPINRKFPADKFDALWA
RVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNLFGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSDG
CVIINFAQKKVLIAGMRYAGEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGDD
EHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSLTQNSRAAYPLEHVAKRSEKNL
GGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRI
RGFGSKVYLVNTGWTGGGYGVGKRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVETNLLNPRNTWADKAA
YDEAAKALAGLFIENFKKFEVSDAIKAAGPKL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=532, Percent_Identity=43.421052631579, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322950, Length=508, Percent_Identity=41.3385826771654, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 55439; Mature: 55308

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQA
CCCCCCCEEECCCHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCHHHHHHCCCCCCCE
AIAWGPINRKFPADKFDALWARVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNL
EEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCEEEEEEEHHHHHHHH
FGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSDGCVIINFAQKKVLIAGMRYA
CCEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCEEEEEHHHHH
GEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCEEEECC
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSL
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEHHCCHHHHHHEECCCCCCCCCEEEE
TQNSRAAYPLEHVAKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTA
ECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHCHHE
LVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFGSKVYLVNTGWTGGGY
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GVGKRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVETNLLNPRNTWADKA
CCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCEEEECCCEEECCCCCCCCCCCCCCHHHHH
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQA
CCCCCCEEECCCHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCHHHHHHCCCCCCCE
AIAWGPINRKFPADKFDALWARVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNL
EEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCEEEEEEEHHHHHHHH
FGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSDGCVIINFAQKKVLIAGMRYA
CCEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCEEEEEHHHHH
GEMKKAMFSVQNFLLPAADVLPMHCAANIGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCEEEECC
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSL
CCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEHHCCHHHHHHEECCCCCCCCCEEEE
TQNSRAAYPLEHVAKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTA
ECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHCHHE
LVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFGSKVYLVNTGWTGGGY
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GVGKRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVETNLLNPRNTWADKA
CCCCCCCCCHHHHHHHHHHCCCEEEEHHHCCEEEECCCEEECCCCCCCCCCCCCCHHHHH
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA