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The map label for this gene is envZ [H]

Identifier: 77456489

GI number: 77456489

Start: 308430

End: 309743

Strand: Direct

Name: envZ [H]

Synonym: Pfl01_0261

Alternate gene names: 77456489

Gene position: 308430-309743 (Clockwise)

Preceding gene: 77456488

Following gene: 77456493

Centisome position: 4.79

GC content: 63.24

Gene sequence:

>1314_bases
ATGAAAACCCCCGTCTGGTTCCCCCAAAGTTTCTTCTCCCGCACCCTGTGGCTGGTGCTGATCGTCGTATTGTTTTCCAA
GGCATTGACCCTGGTTTATCTGTTGATGAACGAGGACGTGCTGGTGGACCGCCAATACAGCCATGGCGTCGCCCTGACGC
TGCGCGCCTACTGGGCTGCCGACGAAGAAAACCGCGCGAAAATCGCCGATGCTGCCACGCTGATCCGGGTGGTGGGCGCC
GGTGTGCCGGAAGGCGAACAGCACTGGCCGTACAGCGAAATCTACCAGCGGCAGATGCAGGCGGAGCTGGGGGCCGACAC
CGAAGTGCGCTTGCGCATGCACTCGCCGCCGGCCTTGTGGGTTCGTGCGCCGAGCCTCGGTGACGGCTGGCTGAAAGTGC
CACTGTATCCACACCCGTTGCGTGGCCAGAAAATCTGGAACGTGCTCGGCTGGTTCCTGGCGATCGGTTTGCTGTCCACG
GCGTCCGCCTGGATTTTCGTCAGCCAGCTCAACCAACCACTGAAGCGTCTGGTCTACGCCGCCCGGCAACTCGGCCAGGG
CCGCAGCGTGCGCCTGCCGATCAGTGACACCCCCAGCGAGATGGCCGAGGTGTATCGCGCCTTCAACCAGATGGCCGAAG
ACGTCGAGCAGGCCGGTCGCGAGCGTGAGCTGATGCTGGCGGGGGTGTCCCATGACTTGCGGACGCCGCTGACTCGTTTG
CGGTTGTCGCTGGAGTTGATGGGCGACCGCACCGACCTGACCGACGACATGGTGCGCGACATCGAAGACATGGACGCGAT
TCTCGATCAGTTCCTCGCGTTCATTCGCGATGGTCGGGACGAGTCGGTGGAGGAGGTGGATCTCAGCGATCTGGTGCGTG
AAGTGGCGGCGCCCTACAACCAGACGGAAGAGAAAGTGCGCCTTCGCCTGGAGCCGATCCAGCCGTTCCCGTTGCGTCGG
GTGTCGATGAAGCGCTTGCTGAACAACCTGATTGGCAACGCGTTGAATCATGCCGGCGGCAGCGTGGACGTCGCGGCCTA
TGTGTCCGGTGACACCAGTGCGCCGTATGTGGTGCTGAGCGTGATGGACCGGGGGGCAGGTATCGATCCTTCCGAACTTG
AGGCGATCTTCAACCCGTTCACCCGTGGCGATCGTGCGCGGGGCGGGAAGGGCACCGGACTGGGCTTGGCGATCGTCAAG
CGGATTGCGTCGATGCACGGCGGCAATGTCGAGCTGCGCAACCGCTCTGGTGGCGGGCTGGAAGCGCGGGTGCGGTTGCC
GCTGGGATTGATGCTGCCAAGAGATGCCGTTTAA

Upstream 100 bases:

>100_bases
GAGCGGGTCTGCGGGAAACGGTCGGAAACGCCGTCCGCGCAGACTCGCAATCCGCAATATGCGAGCATTGCTCGCTCCTG
CAAGTGTGTAGCGGTTATCC

Downstream 100 bases:

>100_bases
CGGCAGCCTCAAGCGGCGAGCTTCAAGCTACAAGCAAAAGCGGCTTTTACTCGCCACTTGCAGCTTGTAGCTCGCCGCTG
TTTTTTATCCCTTGCCTTTT

Product: osmolarity sensor protein envZ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKTPVWFPQSFFSRTLWLVLIVVLFSKALTLVYLLMNEDVLVDRQYSHGVALTLRAYWAADEENRAKIADAATLIRVVGA
GVPEGEQHWPYSEIYQRQMQAELGADTEVRLRMHSPPALWVRAPSLGDGWLKVPLYPHPLRGQKIWNVLGWFLAIGLLST
ASAWIFVSQLNQPLKRLVYAARQLGQGRSVRLPISDTPSEMAEVYRAFNQMAEDVEQAGRERELMLAGVSHDLRTPLTRL
RLSLELMGDRTDLTDDMVRDIEDMDAILDQFLAFIRDGRDESVEEVDLSDLVREVAAPYNQTEEKVRLRLEPIQPFPLRR
VSMKRLLNNLIGNALNHAGGSVDVAAYVSGDTSAPYVVLSVMDRGAGIDPSELEAIFNPFTRGDRARGGKGTGLGLAIVK
RIASMHGGNVELRNRSGGGLEARVRLPLGLMLPRDAV

Sequences:

>Translated_437_residues
MKTPVWFPQSFFSRTLWLVLIVVLFSKALTLVYLLMNEDVLVDRQYSHGVALTLRAYWAADEENRAKIADAATLIRVVGA
GVPEGEQHWPYSEIYQRQMQAELGADTEVRLRMHSPPALWVRAPSLGDGWLKVPLYPHPLRGQKIWNVLGWFLAIGLLST
ASAWIFVSQLNQPLKRLVYAARQLGQGRSVRLPISDTPSEMAEVYRAFNQMAEDVEQAGRERELMLAGVSHDLRTPLTRL
RLSLELMGDRTDLTDDMVRDIEDMDAILDQFLAFIRDGRDESVEEVDLSDLVREVAAPYNQTEEKVRLRLEPIQPFPLRR
VSMKRLLNNLIGNALNHAGGSVDVAAYVSGDTSAPYVVLSVMDRGAGIDPSELEAIFNPFTRGDRARGGKGTGLGLAIVK
RIASMHGGNVELRNRSGGGLEARVRLPLGLMLPRDAV
>Mature_437_residues
MKTPVWFPQSFFSRTLWLVLIVVLFSKALTLVYLLMNEDVLVDRQYSHGVALTLRAYWAADEENRAKIADAATLIRVVGA
GVPEGEQHWPYSEIYQRQMQAELGADTEVRLRMHSPPALWVRAPSLGDGWLKVPLYPHPLRGQKIWNVLGWFLAIGLLST
ASAWIFVSQLNQPLKRLVYAARQLGQGRSVRLPISDTPSEMAEVYRAFNQMAEDVEQAGRERELMLAGVSHDLRTPLTRL
RLSLELMGDRTDLTDDMVRDIEDMDAILDQFLAFIRDGRDESVEEVDLSDLVREVAAPYNQTEEKVRLRLEPIQPFPLRR
VSMKRLLNNLIGNALNHAGGSVDVAAYVSGDTSAPYVVLSVMDRGAGIDPSELEAIFNPFTRGDRARGGKGTGLGLAIVK
RIASMHGGNVELRNRSGGGLEARVRLPLGLMLPRDAV

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=449, Percent_Identity=33.8530066815145, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=32.475884244373, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790346, Length=286, Percent_Identity=30.0699300699301, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788393, Length=282, Percent_Identity=28.3687943262411, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=27.5974025974026, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790861, Length=270, Percent_Identity=28.8888888888889, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=31.404958677686, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788279, Length=309, Percent_Identity=25.5663430420712, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788549, Length=271, Percent_Identity=25.830258302583, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=28.2511210762332, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790551, Length=247, Percent_Identity=29.5546558704453, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786783, Length=249, Percent_Identity=28.1124497991968, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789403, Length=278, Percent_Identity=29.4964028776978, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=255, Percent_Identity=28.6274509803922, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=26.1224489795918, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=23.2758620689655, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=25.2212389380531, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48774; Mature: 48774

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPVWFPQSFFSRTLWLVLIVVLFSKALTLVYLLMNEDVLVDRQYSHGVALTLRAYWAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEECC
DEENRAKIADAATLIRVVGAGVPEGEQHWPYSEIYQRQMQAELGADTEVRLRMHSPPALW
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
VRAPSLGDGWLKVPLYPHPLRGQKIWNVLGWFLAIGLLSTASAWIFVSQLNQPLKRLVYA
EECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQLGQGRSVRLPISDTPSEMAEVYRAFNQMAEDVEQAGRERELMLAGVSHDLRTPLTRL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
RLSLELMGDRTDLTDDMVRDIEDMDAILDQFLAFIRDGRDESVEEVDLSDLVREVAAPYN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCC
QTEEKVRLRLEPIQPFPLRRVSMKRLLNNLIGNALNHAGGSVDVAAYVSGDTSAPYVVLS
CCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEE
VMDRGAGIDPSELEAIFNPFTRGDRARGGKGTGLGLAIVKRIASMHGGNVELRNRSGGGL
EHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
EARVRLPLGLMLPRDAV
EEEEECCEEECCCCCCC
>Mature Secondary Structure
MKTPVWFPQSFFSRTLWLVLIVVLFSKALTLVYLLMNEDVLVDRQYSHGVALTLRAYWAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEEECC
DEENRAKIADAATLIRVVGAGVPEGEQHWPYSEIYQRQMQAELGADTEVRLRMHSPPALW
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
VRAPSLGDGWLKVPLYPHPLRGQKIWNVLGWFLAIGLLSTASAWIFVSQLNQPLKRLVYA
EECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQLGQGRSVRLPISDTPSEMAEVYRAFNQMAEDVEQAGRERELMLAGVSHDLRTPLTRL
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
RLSLELMGDRTDLTDDMVRDIEDMDAILDQFLAFIRDGRDESVEEVDLSDLVREVAAPYN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCC
QTEEKVRLRLEPIQPFPLRRVSMKRLLNNLIGNALNHAGGSVDVAAYVSGDTSAPYVVLS
CCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEE
VMDRGAGIDPSELEAIFNPFTRGDRARGGKGTGLGLAIVKRIASMHGGNVELRNRSGGGL
EHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCC
EARVRLPLGLMLPRDAV
EEEEECCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]