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The map label for this gene is gshA [H]

Identifier: 77456485

GI number: 77456485

Start: 302709

End: 304307

Strand: Reverse

Name: gshA [H]

Synonym: Pfl01_0257

Alternate gene names: 77456485

Gene position: 304307-302709 (Counterclockwise)

Preceding gene: 77456486

Following gene: 77456484

Centisome position: 4.73

GC content: 61.91

Gene sequence:

>1599_bases
GTGAAGGAATCCAAATTGAGCGAACTTCTCAACCGCCGCCTGGCTCTGCTCGGCGAGCGCGCTAACCTCTCTCTGCTTGA
GCAGTGCCTTCACGGTATCGAACGTGAATGCCTGCGCGTGACCGGCGAAGGCCGCCTGGCCCAAACGCCGCACCCTGAAG
CCCTGGGTTCCGCGCTGACCAACGAACAGATCACGACCGACTATTCCGAGTCGCTGCTGGAGTTCATCACGCCCGCCCTG
CCAGACCCGGCCGACACCCTGGCGAGCCTGGACAAGATTCATCGCTTCGCCTACAGCAAGCTCGGCAACGAGTACCTGTG
GAGTCCATCGATGCCGTGCCCGTTGCCGGCCGAGGAAGACATCCCGATCGCCTATTACGGTACCTCCAACATCGGTCAGC
TCAAGTACGTCTACCGCAAGGGTCTGGCCCTGCGTTACGGCAAGACCATGCAGTGCATCGCCGGGATTCACTACAACTTC
TCCCTGCCGGAAAAGCTCTGGCCGCTGCTCAAGGAAGCCGAAGGCTTCGTCGGCACCGACCGGGATTTCCAGTCGTCGTC
CTATATCGCGCTGATCCGTAACTTCCGCCGCTACAGCTGGCTGCTGATGTACCTGTTCGGTGCTTCGCCGGCGCTGGACG
CGGGTTTCCTGCGCGGTCGTGCGCACCAGTTGGAACAGCTGGATCCGGACACCCTGTACCTGCCCTACGCCACCAGCCTG
CGCATGAGCGATCTCGGTTACCAGAGCAACGCCCAGGCCGGTCTGACCCCTTGCTACAACGACCTGGCGAGCTACACCGA
CAGCCTGCGCAAAGCGGTGGCGACGCCGTACGCGCCGTATGTCGAAGTCGGCACGCACCAGGATGGCGAGTGGGTGCAAC
TGAACACCAACATCCTGCAGATCGAAAACGAGTACTACTCGAACATCCGCCCGAAACGCGTGACCTACACCGGCGAGCGT
CCGATCCAGGCCCTGATGGCCCGTGGCATCCAGTACGTCGAAGTGCGCTGCCTGGACATCAACCCGTTCCTGCCAATGGG
CATCGACCTCACCGAGTCGCGCTTCCTCGACGCGTTCCTGCTGTACTGCGCGCTGAACGAAAGCCCGCTGCTGACCAACA
ACTCCTGCGGCAACGCCACCTCCAACTTCCTCAGCGTGGTCAAGGAAGGTCGTCGTCCGGGCCTGCAATTGCAGCGCGAC
GGCCAACCGGTCGAGCTGAAGGAATGGGCCGCCGAACTGCTGGAAAAAATCGCTCCGCTGGCGGCGCTGCTGGATCAAAG
CCACGGCGGCGATGCCCACAGCAAGGCACTCGATGCGCAACTGGCCAAGGTTAAGGATTCGTCCCTGACGCCTTCGGCCC
AGGTGCTTGCGGCGATGGCAGCGCACAAGGAAAGCTTCGCGCAATTCTCCCTGCGCCAGAGCCAGGCCCATGCCGAGTTC
TTCCGCAGCGAGCCGTTGGCGGCTGAGGAACAGGCGAAGTTTGAAGAACTGGCGCGGTCTTCGCTGGCGCAGCAAGCTGA
GCTGGAGCAAAACGAAGTTGGCGATTTCGACGTGTTTGTCGGGTCGTATCAGGCGAGCATTCTGGCGATCAGTAACTGA

Upstream 100 bases:

>100_bases
CCAATGGGGCTTTCATGGCCCGAAAACCAGGCGAAAACGCCAGTTTCAACTTCACCCTTGTAGACCGTCCTGCCCACCCC
CATATTGGGGCGACTGACGC

Downstream 100 bases:

>100_bases
TCGCTTCCATCCGTGATCTTTGATCGTTCCCACGCAGAGCGTGGGAACGATCACCTTCTCTCCCAACTATCACGTTCTCT
CCCTTAGATTTTTCCGCTCA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKESKLSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALTNEQITTDYSESLLEFITPAL
PDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEEDIPIAYYGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEKLWPLLKEAEGFVGTDRDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATSL
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQDGEWVQLNTNILQIENEYYSNIRPKRVTYTGER
PIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFLLYCALNESPLLTNNSCGNATSNFLSVVKEGRRPGLQLQRD
GQPVELKEWAAELLEKIAPLAALLDQSHGGDAHSKALDAQLAKVKDSSLTPSAQVLAAMAAHKESFAQFSLRQSQAHAEF
FRSEPLAAEEQAKFEELARSSLAQQAELEQNEVGDFDVFVGSYQASILAISN

Sequences:

>Translated_532_residues
MKESKLSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALTNEQITTDYSESLLEFITPAL
PDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEEDIPIAYYGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEKLWPLLKEAEGFVGTDRDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATSL
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQDGEWVQLNTNILQIENEYYSNIRPKRVTYTGER
PIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFLLYCALNESPLLTNNSCGNATSNFLSVVKEGRRPGLQLQRD
GQPVELKEWAAELLEKIAPLAALLDQSHGGDAHSKALDAQLAKVKDSSLTPSAQVLAAMAAHKESFAQFSLRQSQAHAEF
FRSEPLAAEEQAKFEELARSSLAQQAELEQNEVGDFDVFVGSYQASILAISN
>Mature_532_residues
MKESKLSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALTNEQITTDYSESLLEFITPAL
PDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEEDIPIAYYGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEKLWPLLKEAEGFVGTDRDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATSL
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQDGEWVQLNTNILQIENEYYSNIRPKRVTYTGER
PIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFLLYCALNESPLLTNNSCGNATSNFLSVVKEGRRPGLQLQRD
GQPVELKEWAAELLEKIAPLAALLDQSHGGDAHSKALDAQLAKVKDSSLTPSAQVLAAMAAHKESFAQFSLRQSQAHAEF
FRSEPLAAEEQAKFEELARSSLAQQAELEQNEVGDFDVFVGSYQASILAISN

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=505, Percent_Identity=41.5841584158416, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59303; Mature: 59303

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESKLSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALT
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHC
NEQITTDYSESLLEFITPALPDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEED
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IPIAYYGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEKLWPLLKEAEGFVGTD
CCEEEECCCCCHHHHHHHHCCCCEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
RDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATSL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQDGEWVQLNTNILQ
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECCCEEE
IENEYYSNIRPKRVTYTGERPIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFL
ECHHHHHCCCCCEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
LYCALNESPLLTNNSCGNATSNFLSVVKEGRRPGLQLQRDGQPVELKEWAAELLEKIAPL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
AALLDQSHGGDAHSKALDAQLAKVKDSSLTPSAQVLAAMAAHKESFAQFSLRQSQAHAEF
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRSEPLAAEEQAKFEELARSSLAQQAELEQNEVGDFDVFVGSYQASILAISN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECC
>Mature Secondary Structure
MKESKLSELLNRRLALLGERANLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGSALT
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHC
NEQITTDYSESLLEFITPALPDPADTLASLDKIHRFAYSKLGNEYLWSPSMPCPLPAEED
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IPIAYYGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEKLWPLLKEAEGFVGTD
CCEEEECCCCCHHHHHHHHCCCCEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
RDFQSSSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRAHQLEQLDPDTLYLPYATSL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQDGEWVQLNTNILQ
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECCCEEE
IENEYYSNIRPKRVTYTGERPIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFL
ECHHHHHCCCCCEEEECCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
LYCALNESPLLTNNSCGNATSNFLSVVKEGRRPGLQLQRDGQPVELKEWAAELLEKIAPL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHH
AALLDQSHGGDAHSKALDAQLAKVKDSSLTPSAQVLAAMAAHKESFAQFSLRQSQAHAEF
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRSEPLAAEEQAKFEELARSSLAQQAELEQNEVGDFDVFVGSYQASILAISN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA