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The map label for this gene is fliY [H]

Identifier: 77456472

GI number: 77456472

Start: 287000

End: 287800

Strand: Reverse

Name: fliY [H]

Synonym: Pfl01_0244

Alternate gene names: 77456472

Gene position: 287800-287000 (Counterclockwise)

Preceding gene: 77456473

Following gene: 77456471

Centisome position: 4.47

GC content: 59.43

Gene sequence:

>801_bases
ATGATTCTTTCCGCACTACGTCGAAATCTGCTGGTAGGTTCGCTGGGCCTGGCACTGAGCGCCGGTCTGCTGGGGCAGGC
GGTTGCCGGTGAGCAACTGCAACAGATCAAGGACAAAGGCGTTATCAACGTCGGCCTGGAAGGCACTTACCCACCGTTCA
GCTTCGTCGATGCCGACGGCAAGCTGTCGGGTTTCGAAGTCGAACTGTCCGAAGCCCTGGCCAAGAAGCTGGGCGTGAAG
GCCAAGATTCAGCCGACCAAATGGGACGGCATCCTCGCAGCCCTGGAATCCAAGCGTCTGGACGTCGTGGTTAACCAAGT
GACCATCTCCGAAGAGCGTAAGAAGAAGTACGACTTCTCCGAGCCGTACACCGTTTCCGGGATTCAGGCACTGGTACTGA
AAGACAAGGCAGCCGCGCTGAACATCAAATCCGCCGCCGACCTGTCCGGCAAGAAAGTCGGCGTCGGTCTGGGCACCAAC
TATGAACAGTGGGTCCGCGCCAACGTGCCGGGTGCCGACGTGCGCACCTACGACGATGATCCGACCAAGTTCGCCGACCT
GAACAACGGCCGTACCGACGCGATCCTGATCGACCGTCTGGCTGCGCTGGAATACGCCCAGAAAGCGCCAAAAACCGTCG
CTGCCGGTGAAGCCTTCTCCCGTCAGGAAGCGGGTATTGCCCTGCGCAAAGGCGAACCTGAACTGCTGGCCGCCGTGAAC
AAGGCCCTCGACGAACTGCGTGCCGACGGCACCCTTGAGAAGCTGTCCAAGAAATACTTCAACGCTGACGTCACCCAATA
A

Upstream 100 bases:

>100_bases
GACGCATAAGCTGCTTTAAGGCAGGATAGCGCTGTCGTCCAATTCCCGGATTCGCCAACTTTCCTTAAGCGTCTTCCATA
AGAAAACACAGGGGCTTGTC

Downstream 100 bases:

>100_bases
TGGAAGAAGCTTTCCAACTCGCTCTGGACTCCGCGCCCTTCCTGTTGAAGGGCGCGTACTACACGGTAATCCTCAGCCTC
GGCGGCATGTTTTTCGGCCT

Product: cystine transporter subunit

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MILSALRRNLLVGSLGLALSAGLLGQAVAGEQLQQIKDKGVINVGLEGTYPPFSFVDADGKLSGFEVELSEALAKKLGVK
AKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFSEPYTVSGIQALVLKDKAAALNIKSAADLSGKKVGVGLGTN
YEQWVRANVPGADVRTYDDDPTKFADLNNGRTDAILIDRLAALEYAQKAPKTVAAGEAFSRQEAGIALRKGEPELLAAVN
KALDELRADGTLEKLSKKYFNADVTQ

Sequences:

>Translated_266_residues
MILSALRRNLLVGSLGLALSAGLLGQAVAGEQLQQIKDKGVINVGLEGTYPPFSFVDADGKLSGFEVELSEALAKKLGVK
AKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFSEPYTVSGIQALVLKDKAAALNIKSAADLSGKKVGVGLGTN
YEQWVRANVPGADVRTYDDDPTKFADLNNGRTDAILIDRLAALEYAQKAPKTVAAGEAFSRQEAGIALRKGEPELLAAVN
KALDELRADGTLEKLSKKYFNADVTQ
>Mature_266_residues
MILSALRRNLLVGSLGLALSAGLLGQAVAGEQLQQIKDKGVINVGLEGTYPPFSFVDADGKLSGFEVELSEALAKKLGVK
AKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFSEPYTVSGIQALVLKDKAAALNIKSAADLSGKKVGVGLGTN
YEQWVRANVPGADVRTYDDDPTKFADLNNGRTDAILIDRLAALEYAQKAPKTVAAGEAFSRQEAGIALRKGEPELLAAVN
KALDELRADGTLEKLSKKYFNADVTQ

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=268, Percent_Identity=62.6865671641791, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI1787085, Length=224, Percent_Identity=30.3571428571429, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=35.0806451612903, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788649, Length=269, Percent_Identity=31.5985130111524, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=30.6451612903226, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=32.618025751073, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1786876, Length=250, Percent_Identity=28, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28516; Mature: 28516

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSALRRNLLVGSLGLALSAGLLGQAVAGEQLQQIKDKGVINVGLEGTYPPFSFVDADG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCC
KLSGFEVELSEALAKKLGVKAKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFS
CCCCEEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCEEEEEEEEECCHHHHHCCCCC
EPYTVSGIQALVLKDKAAALNIKSAADLSGKKVGVGLGTNYEQWVRANVPGADVRTYDDD
CCCEECCEEEEEEECCHHEEECCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCEECCCC
PTKFADLNNGRTDAILIDRLAALEYAQKAPKTVAAGEAFSRQEAGIALRKGEPELLAAVN
CCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEECCCHHHHHHHH
KALDELRADGTLEKLSKKYFNADVTQ
HHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MILSALRRNLLVGSLGLALSAGLLGQAVAGEQLQQIKDKGVINVGLEGTYPPFSFVDADG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCC
KLSGFEVELSEALAKKLGVKAKIQPTKWDGILAALESKRLDVVVNQVTISEERKKKYDFS
CCCCEEEEHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCEEEEEEEEECCHHHHHCCCCC
EPYTVSGIQALVLKDKAAALNIKSAADLSGKKVGVGLGTNYEQWVRANVPGADVRTYDDD
CCCEECCEEEEEEECCHHEEECCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCEECCCC
PTKFADLNNGRTDAILIDRLAALEYAQKAPKTVAAGEAFSRQEAGIALRKGEPELLAAVN
CCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEECCCHHHHHHHH
KALDELRADGTLEKLSKKYFNADVTQ
HHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]