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The map label for this gene is thcD [H]

Identifier: 77456409

GI number: 77456409

Start: 219220

End: 220749

Strand: Reverse

Name: thcD [H]

Synonym: Pfl01_0181

Alternate gene names: 77456409

Gene position: 220749-219220 (Counterclockwise)

Preceding gene: 77456418

Following gene: 77456395

Centisome position: 3.43

GC content: 65.23

Gene sequence:

>1530_bases
ATGACCCTGCACCGTGTCGCCCGTTTCGCCGATGTGCCTGAAGACCGTGGCCTGGAGGTAAAGATCGGCGACTGCAAGAT
CGTGCTGCTGCGCGTTGCCGGCCAATTGCGCGCCTTTCAGGGGCAATGCCCGCACGCCGGCGCGCCGCTGGCGGAAGGCG
CGTTGTGCCACGGCCGGCTGATCTGCCCTTGGCACAAGGCAGCCTTTCGCGCCGAGGATGGCGCCCTGTGCGAGCCTCCG
GCGCTGGACAGCCTCAAGCGTTACCCGCTGGAGCTGCGCGGCGACGAGGTGTGGGTCGATGACCAGCCGCTGCCGGATCC
GCACACCCCGCCGCAGGACGATCCGCGCACCTTCGTCATCGTCGGCGCCGGTGCCGGGGGAACGGCCTGCGCCGCCGCCC
TGCGGGAAAAAGGCTTCGGCGGGCGAATAGTGCTGATCGACCGCGAGCCGGACGCCGGCTACGACCGAACGGTGCTGAGC
AAATTCGTGCTGTCCGGGGAAATGCCGGCGGCGGAAACCCCGCCGCTGCGCGATGAAGACTTTTACCGCGAGCAGCACAT
CAACCGGGTTTCAGGCGAGGTCAGCGGGCTCGATGCCGATACCAAGACTCTTCACCTGAGCGATGGCCGCACACTGAATT
ACAGCGCGGCGGTACTGGCCACCGGCGCAACGCCCAATGCGCTGGAACTGCCCGGCGTCGACCTGCCCCAGGTGTTTGTC
CTGCGCTCCAAAGCCCAGGCGCAATACATCATGGACGCCGCCCAATCCGGTCAGCGGGCGGTGATCATCGGTGACAGTTT
CATCGCGCTCGAATGCGCCTCGGCCCTGCGCCAGCACGGTCTCGAGGTGACGGTGCTGGCGCGTCACGCAATTCCCTTCG
CGGTGCAATTCGGTGAAGCCGTCGGCAAGGCGATCCGGGCACTGCATGAAGAAAACGGCGTGAAATTCATCACCGATCAC
GAAGCCAGGGAGATCACCGGCGACGGCAAGGTCGAGGCGGTGTTGCTCGACAACGGCCTGCGCTTGTCGGCGGATCTGGT
GTTGGCGGGAATCGGCGTGCATCCGGCCACCGAAGCGTTCGGCGATCTTCCGCGCGAGGACGATCAATCATTGTTGGTCG
ATGCCAGCCTGCGCGTCACCGAGAGCCTGTGGGCCATTGGCGATATCGCCACCTTCCCGTTGAACGGCAAGCCTCAACGT
ATCGAACACTGGCGTCTGGCCCAGCAGCACGCGCGGATCGCCGCCGCCAACCTGCTCGGTGGCGAGGAGCATTACCTCGA
CGTGCCGTATTTCTGGACCTGGCATTTCGGCCGCAACTACGACTATCTCGGCCACGCCGAGGGCTGGGATGCGGTCGAAT
TTATCGGCAAACCCGAACAACCGCCGTTTATCGGTCTGTTCGGGAGCAAGGGCGTTGTAGTGGCAGCGGTCGCCTGCGAA
GAGGAACGTGCAATGGCGCTGCTGGCCGAGCGCATGAAGCAACCGCTGCCGATGGATGAAGCCTGGCGGCTGATTCGCGC
CGTCAGGTGA

Upstream 100 bases:

>100_bases
CAAACTATAGCCGTACCGTTCGTGCCGTGGCAGTGGCAAATGGCTGAACCTCGCAATGCGGCCCAAACTCGTAAGAGAAA
TGCCTGTGAGGAGCACGACC

Downstream 100 bases:

>100_bases
TCCGGTTAAGCGGCTCGCGGTTGTCGTCGTCCTCGGCGTGCAGAAAGATCACCCGAGGATGTTCCAGCGGGGCCAGCGGT
GATGGCAGCTCCTGCGGCTG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTLHRVARFADVPEDRGLEVKIGDCKIVLLRVAGQLRAFQGQCPHAGAPLAEGALCHGRLICPWHKAAFRAEDGALCEPP
ALDSLKRYPLELRGDEVWVDDQPLPDPHTPPQDDPRTFVIVGAGAGGTACAAALREKGFGGRIVLIDREPDAGYDRTVLS
KFVLSGEMPAAETPPLRDEDFYREQHINRVSGEVSGLDADTKTLHLSDGRTLNYSAAVLATGATPNALELPGVDLPQVFV
LRSKAQAQYIMDAAQSGQRAVIIGDSFIALECASALRQHGLEVTVLARHAIPFAVQFGEAVGKAIRALHEENGVKFITDH
EAREITGDGKVEAVLLDNGLRLSADLVLAGIGVHPATEAFGDLPREDDQSLLVDASLRVTESLWAIGDIATFPLNGKPQR
IEHWRLAQQHARIAAANLLGGEEHYLDVPYFWTWHFGRNYDYLGHAEGWDAVEFIGKPEQPPFIGLFGSKGVVVAAVACE
EERAMALLAERMKQPLPMDEAWRLIRAVR

Sequences:

>Translated_509_residues
MTLHRVARFADVPEDRGLEVKIGDCKIVLLRVAGQLRAFQGQCPHAGAPLAEGALCHGRLICPWHKAAFRAEDGALCEPP
ALDSLKRYPLELRGDEVWVDDQPLPDPHTPPQDDPRTFVIVGAGAGGTACAAALREKGFGGRIVLIDREPDAGYDRTVLS
KFVLSGEMPAAETPPLRDEDFYREQHINRVSGEVSGLDADTKTLHLSDGRTLNYSAAVLATGATPNALELPGVDLPQVFV
LRSKAQAQYIMDAAQSGQRAVIIGDSFIALECASALRQHGLEVTVLARHAIPFAVQFGEAVGKAIRALHEENGVKFITDH
EAREITGDGKVEAVLLDNGLRLSADLVLAGIGVHPATEAFGDLPREDDQSLLVDASLRVTESLWAIGDIATFPLNGKPQR
IEHWRLAQQHARIAAANLLGGEEHYLDVPYFWTWHFGRNYDYLGHAEGWDAVEFIGKPEQPPFIGLFGSKGVVVAAVACE
EERAMALLAERMKQPLPMDEAWRLIRAVR
>Mature_508_residues
TLHRVARFADVPEDRGLEVKIGDCKIVLLRVAGQLRAFQGQCPHAGAPLAEGALCHGRLICPWHKAAFRAEDGALCEPPA
LDSLKRYPLELRGDEVWVDDQPLPDPHTPPQDDPRTFVIVGAGAGGTACAAALREKGFGGRIVLIDREPDAGYDRTVLSK
FVLSGEMPAAETPPLRDEDFYREQHINRVSGEVSGLDADTKTLHLSDGRTLNYSAAVLATGATPNALELPGVDLPQVFVL
RSKAQAQYIMDAAQSGQRAVIIGDSFIALECASALRQHGLEVTVLARHAIPFAVQFGEAVGKAIRALHEENGVKFITDHE
AREITGDGKVEAVLLDNGLRLSADLVLAGIGVHPATEAFGDLPREDDQSLLVDASLRVTESLWAIGDIATFPLNGKPQRI
EHWRLAQQHARIAAANLLGGEEHYLDVPYFWTWHFGRNYDYLGHAEGWDAVEFIGKPEQPPFIGLFGSKGVVVAAVACEE
ERAMALLAERMKQPLPMDEAWRLIRAVR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=497, Percent_Identity=33.1991951710262, Blast_Score=252, Evalue=7e-67,
Organism=Homo sapiens, GI65787454, Length=497, Percent_Identity=33.1991951710262, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI226437568, Length=497, Percent_Identity=33.1991951710262, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI22202629, Length=410, Percent_Identity=23.6585365853659, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4757732, Length=410, Percent_Identity=23.1707317073171, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788892, Length=373, Percent_Identity=28.686327077748, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789765, Length=320, Percent_Identity=26.875, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=510, Percent_Identity=30.7843137254902, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI281359715, Length=510, Percent_Identity=33.1372549019608, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI281359713, Length=510, Percent_Identity=33.1372549019608, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24639250, Length=510, Percent_Identity=33.1372549019608, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI18543267, Length=510, Percent_Identity=33.1372549019608, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24639252, Length=510, Percent_Identity=33.1372549019608, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24639257, Length=510, Percent_Identity=33.1372549019608, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24585130, Length=488, Percent_Identity=32.9918032786885, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24581020, Length=370, Percent_Identity=24.0540540540541, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573993, Length=370, Percent_Identity=24.0540540540541, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 55449; Mature: 55317

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHRVARFADVPEDRGLEVKIGDCKIVLLRVAGQLRAFQGQCPHAGAPLAEGALCHGRL
CCHHHHHHHHCCCCCCCCEEEECCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
ICPWHKAAFRAEDGALCEPPALDSLKRYPLELRGDEVWVDDQPLPDPHTPPQDDPRTFVI
ECCHHHHHEECCCCCCCCCCCHHHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCEEEE
VGAGAGGTACAAALREKGFGGRIVLIDREPDAGYDRTVLSKFVLSGEMPAAETPPLRDED
EECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHH
FYREQHINRVSGEVSGLDADTKTLHLSDGRTLNYSAAVLATGATPNALELPGVDLPQVFV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCEECCCCCCCHHHE
LRSKAQAQYIMDAAQSGQRAVIIGDSFIALECASALRQHGLEVTVLARHAIPFAVQFGEA
EHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
VGKAIRALHEENGVKFITDHEAREITGDGKVEAVLLDNGLRLSADLVLAGIGVHPATEAF
HHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEEEECCCCEEEEEEEEEECCCCCCHHHH
GDLPREDDQSLLVDASLRVTESLWAIGDIATFPLNGKPQRIEHWRLAQQHARIAAANLLG
CCCCCCCCCEEEEECHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GEEHYLDVPYFWTWHFGRNYDYLGHAEGWDAVEFIGKPEQPPFIGLFGSKGVVVAAVACE
CCCCEEECCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEEECC
EERAMALLAERMKQPLPMDEAWRLIRAVR
HHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TLHRVARFADVPEDRGLEVKIGDCKIVLLRVAGQLRAFQGQCPHAGAPLAEGALCHGRL
CHHHHHHHHCCCCCCCCEEEECCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
ICPWHKAAFRAEDGALCEPPALDSLKRYPLELRGDEVWVDDQPLPDPHTPPQDDPRTFVI
ECCHHHHHEECCCCCCCCCCCHHHHHCCCCEECCCEEEECCCCCCCCCCCCCCCCCEEEE
VGAGAGGTACAAALREKGFGGRIVLIDREPDAGYDRTVLSKFVLSGEMPAAETPPLRDED
EECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHH
FYREQHINRVSGEVSGLDADTKTLHLSDGRTLNYSAAVLATGATPNALELPGVDLPQVFV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEEEEEECCCCCCEECCCCCCCHHHE
LRSKAQAQYIMDAAQSGQRAVIIGDSFIALECASALRQHGLEVTVLARHAIPFAVQFGEA
EHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
VGKAIRALHEENGVKFITDHEAREITGDGKVEAVLLDNGLRLSADLVLAGIGVHPATEAF
HHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEEEECCCCEEEEEEEEEECCCCCCHHHH
GDLPREDDQSLLVDASLRVTESLWAIGDIATFPLNGKPQRIEHWRLAQQHARIAAANLLG
CCCCCCCCCEEEEECHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
GEEHYLDVPYFWTWHFGRNYDYLGHAEGWDAVEFIGKPEQPPFIGLFGSKGVVVAAVACE
CCCCEEECCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCEEEEEEECC
EERAMALLAERMKQPLPMDEAWRLIRAVR
HHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]