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The map label for this gene is oprM [H]

Identifier: 77456402

GI number: 77456402

Start: 208716

End: 210158

Strand: Direct

Name: oprM [H]

Synonym: Pfl01_0174

Alternate gene names: 77456402

Gene position: 208716-210158 (Clockwise)

Preceding gene: 77456401

Following gene: 77456403

Centisome position: 3.24

GC content: 67.78

Gene sequence:

>1443_bases
ATGAGCAGGGCTCTGATGATCGCCGGTTTGGGCATGATGCTGTCGGCCTGCTCGATGGTCGGGCCGGACTACCACGTGCC
CGAAGACGCGGCGATCCAGCGCAAGGATTTCCAGGGCGACCTGGCGGTGGCCGGCAAACCGGTGGTGTCGGCCCCGGTGC
CGGCGGACTGGTGGCGGCTGTATCAGGATCCGCGTCTGGATCAACTGGTGCAGCAGGCCATGGCGTCCAACACCGATCTG
CGGGTGGCGGCGGCGAACCTGTCGCGGGCCCGTGCGCAGGTCGATGAAGCCGAAGCGGCCGGTGGCTGGAGCGGCGGCGT
GAAGATGGGCGCGCAACGCTTGCAGGAATCCGGCGAGGCATTCCTGCTGCCGGAGAAAGTGCCGGTGGCCAACATCGGCG
ATATCGGCATCAGTGCGTCCTATCAGTTCGATCTGTGGGGCACGTTGCAACGCGGGATCGAAGCGGCGAAGGCCAACGCC
GACGCGACCCAGGCCGCGGCCGATACCGCGCGCATCACTCTGGTCGCCGACGTGGTGCGGGCCTACACCCAGGTCTGCGC
GGCCAACGAGGAACAGGAAATCGCCCAGCATTCCCTCGACCTGCAATCCCAGAGCACCAGCCTGATCCAGCGTCTGCGCG
ATGCCGGGCGCGGCGACGAAACCCAGGTGACCCGTTCGCAGACCCAATTCAAATCCTTGCGCGCCGACATGCCGCGTTAT
GAGGCGGCGCGGCAGGCCGGGTTGTTCCGGCTGTCGATGCTGCTGGCCAAGCCTGTCGATCAATTGCCGGCCGGCACCGC
GACCTGTGCCGAGCTGCCGAAAATCGCCCAACTGGTGCCAGTCGGTGACGGCGCCGCGCTGCTCAAGCGCCGGCCGGACA
TCCGCCAGGCCGAGCGGCGTCTGGCGGCCGCGACCGCCGGTATCGGCATCGCCACCGGCGAGCTGTACCCGGACATCAGT
TTCGGCGCGACGATCGGCACCGTCGGCATTCTCGACAACCTGGGCAAACCGTCCACCAACCGCTGGGGCTTCGGCCCGTC
GCTGAGCTGGAGCGTGCCGACCAACGGCGCCCGGGCCCGCATCCGTGAAGCCGAAGCCACGACCCAGGGCGCGCTGGCGC
ATTTCGACGGCGTGGTGCTCAACGCCATCCGCGAAACCCAGACAGGTCTGTCCCAGTACTCCGCGCTGCTGCAACGCCGC
GATGCACTGGCCGATGCCGAGCAGTCGGCAAAACTGGCGGCGGACCAGACGCACCGCTTCTTCCAGGCCGGTCGCGAGTC
GTTCCTTGCCGACCTGCAGGCCACCCGCACCTACACCGATGTCACCGCGCAACTGGCCGCCGCCAACACCCAGGTTGCCA
TGAGCCAGATCGATTTGTTCCTCGCCCTGGGCGGCGGTTGGGAAAGCGGACGAACGCAAGCCTCGAGCGCCAGCAAACCC
TGA

Upstream 100 bases:

>100_bases
TGCGCATTGCCTTCGACGATGTGCCGGATGACTTCCGCATGATCGCCGGGCGTACCGCCACTGTGTCGATCATCGACGAT
CAACCGCAGGAGCCCGCCAA

Downstream 100 bases:

>100_bases
GGCCGTTGCTATGCTTTGAAGTATTGAAAGGGCGCCCCGAACCAGGCGCCTTTTCAGTGCACATCGCTAGCGCAGGTCTA
GGGGAAACCAATAATGAAAA

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRALMIAGLGMMLSACSMVGPDYHVPEDAAIQRKDFQGDLAVAGKPVVSAPVPADWWRLYQDPRLDQLVQQAMASNTDL
RVAAANLSRARAQVDEAEAAGGWSGGVKMGAQRLQESGEAFLLPEKVPVANIGDIGISASYQFDLWGTLQRGIEAAKANA
DATQAAADTARITLVADVVRAYTQVCAANEEQEIAQHSLDLQSQSTSLIQRLRDAGRGDETQVTRSQTQFKSLRADMPRY
EAARQAGLFRLSMLLAKPVDQLPAGTATCAELPKIAQLVPVGDGAALLKRRPDIRQAERRLAAATAGIGIATGELYPDIS
FGATIGTVGILDNLGKPSTNRWGFGPSLSWSVPTNGARARIREAEATTQGALAHFDGVVLNAIRETQTGLSQYSALLQRR
DALADAEQSAKLAADQTHRFFQAGRESFLADLQATRTYTDVTAQLAAANTQVAMSQIDLFLALGGGWESGRTQASSASKP

Sequences:

>Translated_480_residues
MSRALMIAGLGMMLSACSMVGPDYHVPEDAAIQRKDFQGDLAVAGKPVVSAPVPADWWRLYQDPRLDQLVQQAMASNTDL
RVAAANLSRARAQVDEAEAAGGWSGGVKMGAQRLQESGEAFLLPEKVPVANIGDIGISASYQFDLWGTLQRGIEAAKANA
DATQAAADTARITLVADVVRAYTQVCAANEEQEIAQHSLDLQSQSTSLIQRLRDAGRGDETQVTRSQTQFKSLRADMPRY
EAARQAGLFRLSMLLAKPVDQLPAGTATCAELPKIAQLVPVGDGAALLKRRPDIRQAERRLAAATAGIGIATGELYPDIS
FGATIGTVGILDNLGKPSTNRWGFGPSLSWSVPTNGARARIREAEATTQGALAHFDGVVLNAIRETQTGLSQYSALLQRR
DALADAEQSAKLAADQTHRFFQAGRESFLADLQATRTYTDVTAQLAAANTQVAMSQIDLFLALGGGWESGRTQASSASKP
>Mature_479_residues
SRALMIAGLGMMLSACSMVGPDYHVPEDAAIQRKDFQGDLAVAGKPVVSAPVPADWWRLYQDPRLDQLVQQAMASNTDLR
VAAANLSRARAQVDEAEAAGGWSGGVKMGAQRLQESGEAFLLPEKVPVANIGDIGISASYQFDLWGTLQRGIEAAKANAD
ATQAAADTARITLVADVVRAYTQVCAANEEQEIAQHSLDLQSQSTSLIQRLRDAGRGDETQVTRSQTQFKSLRADMPRYE
AARQAGLFRLSMLLAKPVDQLPAGTATCAELPKIAQLVPVGDGAALLKRRPDIRQAERRLAAATAGIGIATGELYPDISF
GATIGTVGILDNLGKPSTNRWGFGPSLSWSVPTNGARARIREAEATTQGALAHFDGVVLNAIRETQTGLSQYSALLQRRD
ALADAEQSAKLAADQTHRFFQAGRESFLADLQATRTYTDVTAQLAAANTQVAMSQIDLFLALGGGWESGRTQASSASKP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=476, Percent_Identity=25.8403361344538, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI2367348, Length=493, Percent_Identity=22.920892494929, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51119; Mature: 50988

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRALMIAGLGMMLSACSMVGPDYHVPEDAAIQRKDFQGDLAVAGKPVVSAPVPADWWRL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCHHHHHH
YQDPRLDQLVQQAMASNTDLRVAAANLSRARAQVDEAEAAGGWSGGVKMGAQRLQESGEA
HCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCE
FLLPEKVPVANIGDIGISASYQFDLWGTLQRGIEAAKANADATQAAADTARITLVADVVR
EECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AYTQVCAANEEQEIAQHSLDLQSQSTSLIQRLRDAGRGDETQVTRSQTQFKSLRADMPRY
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
EAARQAGLFRLSMLLAKPVDQLPAGTATCAELPKIAQLVPVGDGAALLKRRPDIRQAERR
HHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHH
LAAATAGIGIATGELYPDISFGATIGTVGILDNLGKPSTNRWGFGPSLSWSVPTNGARAR
HHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCHHHH
IREAEATTQGALAHFDGVVLNAIRETQTGLSQYSALLQRRDALADAEQSAKLAADQTHRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQAGRESFLADLQATRTYTDVTAQLAAANTQVAMSQIDLFLALGGGWESGRTQASSASKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRALMIAGLGMMLSACSMVGPDYHVPEDAAIQRKDFQGDLAVAGKPVVSAPVPADWWRL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCHHHHHH
YQDPRLDQLVQQAMASNTDLRVAAANLSRARAQVDEAEAAGGWSGGVKMGAQRLQESGEA
HCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCE
FLLPEKVPVANIGDIGISASYQFDLWGTLQRGIEAAKANADATQAAADTARITLVADVVR
EECCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AYTQVCAANEEQEIAQHSLDLQSQSTSLIQRLRDAGRGDETQVTRSQTQFKSLRADMPRY
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
EAARQAGLFRLSMLLAKPVDQLPAGTATCAELPKIAQLVPVGDGAALLKRRPDIRQAERR
HHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHH
LAAATAGIGIATGELYPDISFGATIGTVGILDNLGKPSTNRWGFGPSLSWSVPTNGARAR
HHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCHHHH
IREAEATTQGALAHFDGVVLNAIRETQTGLSQYSALLQRRDALADAEQSAKLAADQTHRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQAGRESFLADLQATRTYTDVTAQLAAANTQVAMSQIDLFLALGGGWESGRTQASSASKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]