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The map label for this gene is kinB [H]

Identifier: 77456275

GI number: 77456275

Start: 50798

End: 52588

Strand: Direct

Name: kinB [H]

Synonym: Pfl01_0047

Alternate gene names: 77456275

Gene position: 50798-52588 (Clockwise)

Preceding gene: 77456274

Following gene: 77456283

Centisome position: 0.79

GC content: 61.36

Gene sequence:

>1791_bases
ATGAAACTGGCGATGAAGTTGCGGACTCGCTTGTTCCTGAGCATTTCCGCGCTGATCACCGTGGCCTTGCTCGGGTTGAT
GCTCGGGCTGGTCAGTGTGATGCAGATGGCCGGCACTCAGGAGGCGCTGATCCGCAACAACTTTGTCACCCTGGATCTGG
GGCTCAAGCTGCGCCAGACACTCGGCGATCAACTGATCATGATGCTCGCCGAAAAGCCCGACCCCGTGGCGTTCGAAGCG
TCCAAACAGCATTACTTCCAGTTGCTCGACGAGGGCATCGCCCAAGAGCAGAAAGACGACGATCGCCAGTATGGATTCAG
TCAGGCCAAAGCCGATTACCTGAGCTTCCTCCAGGCGTTCGACATCTCCCGCGACCCGGCTGCTGCCCTGAGCGGCGGTG
GCGACTTGCGTGAGCGCTTCAACACCTTGCGCAACGGGTTGATCACCGAACACAAACACGCGCTGAACAACATCAATTCG
GTCCAGCACGCCGCTCGCGACCGCGCGTTGCTGATCGCTGGCCTGTTGGGACTGGTGGGGCTGGCGGTGCTGATCATTGG
CTTCGTTACCGCCCAGGGCATTGCCCGGCGTTTTGGTGCGCCGATCGAAGCGCTGGCCAAAGCGGCGGACAATATCGGCC
AAGGCAATTTCGAAGTGACCCTGCCAGTGTCCTCGGCGATGGAGATCAACCTCCTGACCCGGCGCTTCGGGCTCATGGCC
GAAGCCCTGCGCGAACATCAGGCGACCAACGTCGACGAATTGCTTGCCGGCCAGCAGCGTCTGCAAGCGGTACTCGACAG
CATCGATGACGGCTTGTTGATGATCGACCGTCAGGGTCGACTGGAACACTTGAACCCGGTGGCCCAGCGGCAACTGGGCT
GGGACAGCGATCGCTTCGGCGAGGGCCTCGGCACGGCGCTTCAGCGACCCGAGCTGGATGCACAACTGCAATTGGTACTG
CGCGGCGGTACCCTTGAACGGGCACCGGAAGATTTGAGCATCGAGGTTGATGGCGAATCGCGCCTGCTGACCTACAGCCT
GACGCCCGTCAGTCATACCCAGGGACATATCCTCGGTGCTGTGATGGTTTTGCATGACGTCACCGAGCAACGCGCCTTCG
AACGGGTACGCAGCGAGTTTGTCCTGCGTGCGTCTCACGAATTGCGCACACCGGTCACGGGCATGCACATGGCGTTCGGG
CTGTTTCGCGAACGGGCCAAGTTTCCTGAGGAGTCTCGGGAGTCCGACCTGCTCGACACGGTTAATGAGGAAATGCAGCG
CCTGATGCAGCTGATCAACGATCTGCTGAACTTCTCGCGCTATCAGAGTGGTTTGCAAAAACTTACGTTGGCGCCTTGCT
CCATCGAGGAACTGCTGGAGCGGGCGCGTTTGCGTTTCGCCGATTCAGCAGCGCAGAAGGGCGTTGCCCTGAATGTCGAA
GCGCAGGGCTCGCTGCCACGCTTGCAGGCGGATCAGGCGCAGCTTGATCGAGTCCTCGACAACCTGATCGACAACGCCTT
GCGCCACACCGCGCGCGACGGGCTGATCCGCTTGCAGGCCCGGCGCCATGGCGAGCGGGTGATCATCAGCGTCGAAGACA
ACGGTGAAGGGATTGCCTACGGTCAGCAGGGGCGGATCTTCGAACCCTTCGTCCAGGTCGGCAGGAAAAAGGGCGGGGCC
GGGCTCGGTTTGGCGCTGTGCAAGGAAATCGTCCAGCTGCACGGTGGGCGAATGGGTGTTTACTCGCGGCCGGGGCAGGG
CACGCAGTTCTACATGGCGCTGGCGGTCTAG

Upstream 100 bases:

>100_bases
GCGACCGCCGGGACCCTCGATCAGGCTGCCAAGACTCTGGGGATCGACGCCTCGACCCTGTATCGCAAGCGCAAGCAGTA
CAACCTGTGAGCATCCGCCG

Downstream 100 bases:

>100_bases
GCCTCATCGTCCAACCGGCGCCCGGCGAGTCGCCGTCCACGTGTTATCAGCTCGATGAACTGCACCGCGCTCAAGGCGTG
GGCGAACAGCCAGCCTTGGC

Product: alginate biosynthesis sensor protein KinB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKLAMKLRTRLFLSISALITVALLGLMLGLVSVMQMAGTQEALIRNNFVTLDLGLKLRQTLGDQLIMMLAEKPDPVAFEA
SKQHYFQLLDEGIAQEQKDDDRQYGFSQAKADYLSFLQAFDISRDPAAALSGGGDLRERFNTLRNGLITEHKHALNNINS
VQHAARDRALLIAGLLGLVGLAVLIIGFVTAQGIARRFGAPIEALAKAADNIGQGNFEVTLPVSSAMEINLLTRRFGLMA
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGRLEHLNPVAQRQLGWDSDRFGEGLGTALQRPELDAQLQLVL
RGGTLERAPEDLSIEVDGESRLLTYSLTPVSHTQGHILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFG
LFRERAKFPEESRESDLLDTVNEEMQRLMQLINDLLNFSRYQSGLQKLTLAPCSIEELLERARLRFADSAAQKGVALNVE
AQGSLPRLQADQAQLDRVLDNLIDNALRHTARDGLIRLQARRHGERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGA
GLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALAV

Sequences:

>Translated_596_residues
MKLAMKLRTRLFLSISALITVALLGLMLGLVSVMQMAGTQEALIRNNFVTLDLGLKLRQTLGDQLIMMLAEKPDPVAFEA
SKQHYFQLLDEGIAQEQKDDDRQYGFSQAKADYLSFLQAFDISRDPAAALSGGGDLRERFNTLRNGLITEHKHALNNINS
VQHAARDRALLIAGLLGLVGLAVLIIGFVTAQGIARRFGAPIEALAKAADNIGQGNFEVTLPVSSAMEINLLTRRFGLMA
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGRLEHLNPVAQRQLGWDSDRFGEGLGTALQRPELDAQLQLVL
RGGTLERAPEDLSIEVDGESRLLTYSLTPVSHTQGHILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFG
LFRERAKFPEESRESDLLDTVNEEMQRLMQLINDLLNFSRYQSGLQKLTLAPCSIEELLERARLRFADSAAQKGVALNVE
AQGSLPRLQADQAQLDRVLDNLIDNALRHTARDGLIRLQARRHGERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGA
GLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALAV
>Mature_596_residues
MKLAMKLRTRLFLSISALITVALLGLMLGLVSVMQMAGTQEALIRNNFVTLDLGLKLRQTLGDQLIMMLAEKPDPVAFEA
SKQHYFQLLDEGIAQEQKDDDRQYGFSQAKADYLSFLQAFDISRDPAAALSGGGDLRERFNTLRNGLITEHKHALNNINS
VQHAARDRALLIAGLLGLVGLAVLIIGFVTAQGIARRFGAPIEALAKAADNIGQGNFEVTLPVSSAMEINLLTRRFGLMA
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGRLEHLNPVAQRQLGWDSDRFGEGLGTALQRPELDAQLQLVL
RGGTLERAPEDLSIEVDGESRLLTYSLTPVSHTQGHILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFG
LFRERAKFPEESRESDLLDTVNEEMQRLMQLINDLLNFSRYQSGLQKLTLAPCSIEELLERARLRFADSAAQKGVALNVE
AQGSLPRLQADQAQLDRVLDNLIDNALRHTARDGLIRLQARRHGERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGA
GLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALAV

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=423, Percent_Identity=27.4231678486998, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1786600, Length=346, Percent_Identity=28.6127167630058, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=30.379746835443, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081816, Length=225, Percent_Identity=31.1111111111111, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI145693157, Length=220, Percent_Identity=32.2727272727273, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790436, Length=212, Percent_Identity=32.5471698113208, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=33.1838565022422, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788713, Length=293, Percent_Identity=26.6211604095563, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=28.936170212766, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788393, Length=274, Percent_Identity=28.4671532846715, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI48994928, Length=243, Percent_Identity=27.9835390946502, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=28.125, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790861, Length=202, Percent_Identity=30.6930693069307, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787894, Length=210, Percent_Identity=29.5238095238095, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790567, Length=348, Percent_Identity=28.1609195402299, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=29.4605809128631, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790551, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788381, Length=192, Percent_Identity=31.7708333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65828; Mature: 65828

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAMKLRTRLFLSISALITVALLGLMLGLVSVMQMAGTQEALIRNNFVTLDLGLKLRQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCHHHHHH
LGDQLIMMLAEKPDPVAFEASKQHYFQLLDEGIAQEQKDDDRQYGFSQAKADYLSFLQAF
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHH
DISRDPAAALSGGGDLRERFNTLRNGLITEHKHALNNINSVQHAARDRALLIAGLLGLVG
CCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVLIIGFVTAQGIARRFGAPIEALAKAADNIGQGNFEVTLPVSSAMEINLLTRRFGLMA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGRLEHLNPVAQRQLGWDSDRFG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHCCCCCHHHC
EGLGTALQRPELDAQLQLVLRGGTLERAPEDLSIEVDGESRLLTYSLTPVSHTQGHILGA
CHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHH
VMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLFRERAKFPEESRESDLLDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VNEEMQRLMQLINDLLNFSRYQSGLQKLTLAPCSIEELLERARLRFADSAAQKGVALNVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
AQGSLPRLQADQAQLDRVLDNLIDNALRHTARDGLIRLQARRHGERVIISVEDNGEGIAY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCEEEEEEECCCCEEEE
GQQGRIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALAV
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MKLAMKLRTRLFLSISALITVALLGLMLGLVSVMQMAGTQEALIRNNFVTLDLGLKLRQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCHHHHHH
LGDQLIMMLAEKPDPVAFEASKQHYFQLLDEGIAQEQKDDDRQYGFSQAKADYLSFLQAF
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHH
DISRDPAAALSGGGDLRERFNTLRNGLITEHKHALNNINSVQHAARDRALLIAGLLGLVG
CCCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVLIIGFVTAQGIARRFGAPIEALAKAADNIGQGNFEVTLPVSSAMEINLLTRRFGLMA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
EALREHQATNVDELLAGQQRLQAVLDSIDDGLLMIDRQGRLEHLNPVAQRQLGWDSDRFG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHCCCCCHHHC
EGLGTALQRPELDAQLQLVLRGGTLERAPEDLSIEVDGESRLLTYSLTPVSHTQGHILGA
CHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHH
VMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLFRERAKFPEESRESDLLDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VNEEMQRLMQLINDLLNFSRYQSGLQKLTLAPCSIEELLERARLRFADSAAQKGVALNVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEE
AQGSLPRLQADQAQLDRVLDNLIDNALRHTARDGLIRLQARRHGERVIISVEDNGEGIAY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCEEEEEEECCCCEEEE
GQQGRIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVYSRPGQGTQFYMALAV
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]