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The map label for this gene is citN [H]

Identifier: 77456259

GI number: 77456259

Start: 32899

End: 34206

Strand: Direct

Name: citN [H]

Synonym: Pfl01_0031

Alternate gene names: 77456259

Gene position: 32899-34206 (Clockwise)

Preceding gene: 77456257

Following gene: 77456260

Centisome position: 0.51

GC content: 61.24

Gene sequence:

>1308_bases
ATGCTGACTTTCCTTGGCTTCGCCATGGTCATCACGTTCATGTTCCTGATCATGACCAAGCGCCTGTCTGCGCTGATCGC
TCTGATCATCATCCCGATCCTGTTCGCCCTGTTCGGTGGTTTCGCGCCGAAGATCGGCCCGATGATGCTCGAAGGTATTA
CCAAGCTTGCGCCGACCGGCGTGATGCTGATGTTCGCGATTCTGTATTTCGCCCTGATGATCGACTCCGGACTGTTCGAC
CCGGCCGTGCGCAAGATCCTCAAACTGGTCAAGGGCGACCCGCTGAAAGTTTCGGTCGGCACCGCCGTACTGGCGCTCGT
CGTTTCCCTCGATGGTGACGGCGCGACCACTTACATGATCTGCGTGGCCGCGATGCTGCCGCTGTACAGCCGCATCGGCA
TGAGCCCGCGGATCATGGCCGGTCTGATCATCCTCGCCGGCGGCGTGATGAACATGACCCCATGGGGTGGCCCGACCGCC
CGTGCGGCGAGCGCGCTGCACGTGGACCCGTCGGACATCTTCGTACCGATGATCCCGGCGATGGCTGCCGGTGTGGTAGC
GATCCTGATCATTGCCTACTTCTATGGCAAACGTGAGCGTGCGCGTCTGGGCGAGCTGCACCTGATCGGCGACGAAATCG
ATCACAGCGAAATCAGCGTCTCGCAATTCCCGGATGCCCGCCGTCCGAAACTGATCTGGTTCAACGGCCTGCTGACCCTG
GCGCTGATGTGCACCCTGATCGCCGGCCTGCTGCCGCTGCCGGTGCTGTTCATGGTGGCGTTCAGTATTGCCATGATCGT
CAACTACCCTTGCCTGCAAATGCAGAAGGATCGCGTCGCGGCCCACGCCGGCAGCGTGCTGTCGGTGGTCAGTCTGATCT
TCGCGGCGGGCATTTTCACCGGTATCCTGTCGGGCACCGGCATGGTCGACGCGATGTCGAAAAGCCTGCTGGCGGTGATC
CCTGATTTCCTCGGCCCGTACCTGGCGGTGATCACGGCGCTGGTGAGCATGCCGTTCACGTTCTTCATGTCGAACGACGC
GTTCTACTACGGTGTGTTGCCGGTGTTGTCCGAAGCGGCCAGCCATTACGGCATCACCGCAGTGGAAATGGCCCGTGCCT
CGATCGTCGGTCAGCCGGTTCACCTGCTGAGCCCACTGGTGCCGTCGACATACCTGCTGGTGGCACTGGCGGGGATCGAG
TTCGGCGATCATCAGCGTTTCACCCTGAAGTGGGCAGTGCTGGTCTGCCTGTGCATACTGGTCGCTGCCTTGCTGTTGGG
CACTTTCCCGCTGTTCAGCACGTTGTAA

Upstream 100 bases:

>100_bases
CTTCGCTGCAACTCCGCCCGTACGCCTCGTGCTTCCCGTTCTGCGCTGATCCCGCCCGATCCGCTCACTTACCTAGCCGC
GCTACGCCTGGAGTATTCCC

Downstream 100 bases:

>100_bases
CGGCACCAAACTCACCAGGACGCCAGCTGATTGCTGGCGTCCTGGTTTAACACTCGCTCAAAGGAATACACATGGAATGG
CTGACCAACCCTGAGATCTG

Product: citrate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLTFLGFAMVITFMFLIMTKRLSALIALIIIPILFALFGGFAPKIGPMMLEGITKLAPTGVMLMFAILYFALMIDSGLFD
PAVRKILKLVKGDPLKVSVGTAVLALVVSLDGDGATTYMICVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTA
RAASALHVDPSDIFVPMIPAMAAGVVAILIIAYFYGKRERARLGELHLIGDEIDHSEISVSQFPDARRPKLIWFNGLLTL
ALMCTLIAGLLPLPVLFMVAFSIAMIVNYPCLQMQKDRVAAHAGSVLSVVSLIFAAGIFTGILSGTGMVDAMSKSLLAVI
PDFLGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAASHYGITAVEMARASIVGQPVHLLSPLVPSTYLLVALAGIE
FGDHQRFTLKWAVLVCLCILVAALLLGTFPLFSTL

Sequences:

>Translated_435_residues
MLTFLGFAMVITFMFLIMTKRLSALIALIIIPILFALFGGFAPKIGPMMLEGITKLAPTGVMLMFAILYFALMIDSGLFD
PAVRKILKLVKGDPLKVSVGTAVLALVVSLDGDGATTYMICVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTA
RAASALHVDPSDIFVPMIPAMAAGVVAILIIAYFYGKRERARLGELHLIGDEIDHSEISVSQFPDARRPKLIWFNGLLTL
ALMCTLIAGLLPLPVLFMVAFSIAMIVNYPCLQMQKDRVAAHAGSVLSVVSLIFAAGIFTGILSGTGMVDAMSKSLLAVI
PDFLGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAASHYGITAVEMARASIVGQPVHLLSPLVPSTYLLVALAGIE
FGDHQRFTLKWAVLVCLCILVAALLLGTFPLFSTL
>Mature_435_residues
MLTFLGFAMVITFMFLIMTKRLSALIALIIIPILFALFGGFAPKIGPMMLEGITKLAPTGVMLMFAILYFALMIDSGLFD
PAVRKILKLVKGDPLKVSVGTAVLALVVSLDGDGATTYMICVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTA
RAASALHVDPSDIFVPMIPAMAAGVVAILIIAYFYGKRERARLGELHLIGDEIDHSEISVSQFPDARRPKLIWFNGLLTL
ALMCTLIAGLLPLPVLFMVAFSIAMIVNYPCLQMQKDRVAAHAGSVLSVVSLIFAAGIFTGILSGTGMVDAMSKSLLAVI
PDFLGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAASHYGITAVEMARASIVGQPVHLLSPLVPSTYLLVALAGIE
FGDHQRFTLKWAVLVCLCILVAALLLGTFPLFSTL

Specific function: Transports the free citrate anion. Probably cotransports citrate and at least three or four protons. The citrate uptake is inhibited by the presence of magnesium ions [H]

COG id: COG2851

COG function: function code C; H+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014738
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 46515; Mature: 46515

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFLGFAMVITFMFLIMTKRLSALIALIIIPILFALFGGFAPKIGPMMLEGITKLAPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
VMLMFAILYFALMIDSGLFDPAVRKILKLVKGDPLKVSVGTAVLALVVSLDGDGATTYMI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHHH
CVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTARAASALHVDPSDIFVPMIPA
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCEEECCHHHHHHHHHHH
MAAGVVAILIIAYFYGKRERARLGELHLIGDEIDHSEISVSQFPDARRPKLIWFNGLLTL
HHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCCCCCCCCHHHCCCCCCCEEEEHHHHHHH
ALMCTLIAGLLPLPVLFMVAFSIAMIVNYPCLQMQKDRVAAHAGSVLSVVSLIFAAGIFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILSGTGMVDAMSKSLLAVIPDFLGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHH
SHYGITAVEMARASIVGQPVHLLSPLVPSTYLLVALAGIEFGDHQRFTLKWAVLVCLCIL
HHCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VAALLLGTFPLFSTL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTFLGFAMVITFMFLIMTKRLSALIALIIIPILFALFGGFAPKIGPMMLEGITKLAPTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
VMLMFAILYFALMIDSGLFDPAVRKILKLVKGDPLKVSVGTAVLALVVSLDGDGATTYMI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCHHHHHH
CVAAMLPLYSRIGMSPRIMAGLIILAGGVMNMTPWGGPTARAASALHVDPSDIFVPMIPA
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCEEECCHHHHHHHHHHH
MAAGVVAILIIAYFYGKRERARLGELHLIGDEIDHSEISVSQFPDARRPKLIWFNGLLTL
HHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCCCCCCCCHHHCCCCCCCEEEEHHHHHHH
ALMCTLIAGLLPLPVLFMVAFSIAMIVNYPCLQMQKDRVAAHAGSVLSVVSLIFAAGIFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILSGTGMVDAMSKSLLAVIPDFLGPYLAVITALVSMPFTFFMSNDAFYYGVLPVLSEAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHHHHHHH
SHYGITAVEMARASIVGQPVHLLSPLVPSTYLLVALAGIEFGDHQRFTLKWAVLVCLCIL
HHCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VAALLLGTFPLFSTL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377; 7704256; 8892821 [H]