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The map label for this gene is ampR [H]

Identifier: 77456255

GI number: 77456255

Start: 30147

End: 31097

Strand: Direct

Name: ampR [H]

Synonym: Pfl01_0027

Alternate gene names: 77456255

Gene position: 30147-31097 (Clockwise)

Preceding gene: 77456251

Following gene: 77456257

Centisome position: 0.47

GC content: 60.88

Gene sequence:

>951_bases
ATGTATGACGCCCTCGGTGATTTGTCGCTGGACCTGCTGCGCGCCTTCGAGGCGGCGGCCCGTCATCGCAGTTTTACCGC
TGCGGCGGTGGAGCTCGGCACGACCCAACCGGCCATCAGCCAGCAGATCAAGCGGCTTGAAGAACAACTCGGCACGCGGC
TGTTCGACCGCATCTACCGGGGCATCGAACTGACCGAGGTCGGATCGATCCTGTTCGAGCAGGTTACCCTCGGTTTGCAG
AATATCGACGCAGGATTGAGCGCGATCAGCACGCAGCAGCAACATGAAGTACTGCAGGTCGCCACCGATTTCGCCTTCGC
CGCGTATTGGCTGATGCCGCGCCTGCACCGCTTCCATGAAGCTTATCCACAGGTGGACGTGAGCCTGGTCACCAGCGAGC
GCAATCACAACATGCTGCGTCCGGATATCGACGTTGCCGTGCTGTTCGGCGACGGTCGCTTCAAGCAGGGCGAAAGTCAC
TGGTTGTTCAGCGAAGAAGTGTTTCCGGTGTGCAGTCCGCAATTGCTCAAGGATTGCTCCCTGCCCTTGCCGGCCCAGGC
ATTGATGGAGTTTCCGCTGCTGCACCTGCGCGGGGAAAACAGCAGTAACTGGTTCGACTGGAGCGGCCTGTTCCGCGCGC
TGAACATCAGTACGCCGCCGGCCCCGGGCCAGTTGCGCTTCGACAATTACACTTTGTTGATTCAAGCGGCGATTGGCGGT
CAGGGCGTGGCAATTGGCTGGCGACACCTTGTGGATAACTTGCTCACTCAGGGTCTGTTGTGCCGGCCGATCGCTGAGAC
CACCCTGTCGCGCCTCGGTTATTACGTGGTGCTGCCGCAACGCAAACGCCGGGGCGCGTTGATCCAGACGTTCGTGGATT
GGCTGATGGCCGAACAGGCGAGCAGCGCCGAATCCCTGGCCGGGCTACCGTTGCCGTCGATTGCTGTGTAA

Upstream 100 bases:

>100_bases
CCATAGTGAATATTTATGCGACATTGCTGCGATCGAGCATGGATTCCCCGCGATATCCGGTAAAGCCCGCGCCGGACAAT
GACTAGGATAAGCACAGCTT

Downstream 100 bases:

>100_bases
CGGATCAAGAAGCGGCGACAAAATCCACGTGCTGGCGCAGATGCAAATTCAGCCGCAGCTCATCGGCAATGCCCACCGCC
ACCCGGTTCAGGCGCTCCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MYDALGDLSLDLLRAFEAAARHRSFTAAAVELGTTQPAISQQIKRLEEQLGTRLFDRIYRGIELTEVGSILFEQVTLGLQ
NIDAGLSAISTQQQHEVLQVATDFAFAAYWLMPRLHRFHEAYPQVDVSLVTSERNHNMLRPDIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLKDCSLPLPAQALMEFPLLHLRGENSSNWFDWSGLFRALNISTPPAPGQLRFDNYTLLIQAAIGG
QGVAIGWRHLVDNLLTQGLLCRPIAETTLSRLGYYVVLPQRKRRGALIQTFVDWLMAEQASSAESLAGLPLPSIAV

Sequences:

>Translated_316_residues
MYDALGDLSLDLLRAFEAAARHRSFTAAAVELGTTQPAISQQIKRLEEQLGTRLFDRIYRGIELTEVGSILFEQVTLGLQ
NIDAGLSAISTQQQHEVLQVATDFAFAAYWLMPRLHRFHEAYPQVDVSLVTSERNHNMLRPDIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLKDCSLPLPAQALMEFPLLHLRGENSSNWFDWSGLFRALNISTPPAPGQLRFDNYTLLIQAAIGG
QGVAIGWRHLVDNLLTQGLLCRPIAETTLSRLGYYVVLPQRKRRGALIQTFVDWLMAEQASSAESLAGLPLPSIAV
>Mature_316_residues
MYDALGDLSLDLLRAFEAAARHRSFTAAAVELGTTQPAISQQIKRLEEQLGTRLFDRIYRGIELTEVGSILFEQVTLGLQ
NIDAGLSAISTQQQHEVLQVATDFAFAAYWLMPRLHRFHEAYPQVDVSLVTSERNHNMLRPDIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLKDCSLPLPAQALMEFPLLHLRGENSSNWFDWSGLFRALNISTPPAPGQLRFDNYTLLIQAAIGG
QGVAIGWRHLVDNLLTQGLLCRPIAETTLSRLGYYVVLPQRKRRGALIQTFVDWLMAEQASSAESLAGLPLPSIAV

Specific function: This protein is a positive regulator of gene expression of cephalosporinase (AmpC) [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=34.7972972972973, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=32.996632996633, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1788706, Length=304, Percent_Identity=31.5789473684211, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=27.3648648648649, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=35.8024691358025, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=26.3513513513513, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=120, Percent_Identity=38.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=24.124513618677, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=123, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=131, Percent_Identity=29.7709923664122, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789440, Length=225, Percent_Identity=22.6666666666667, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35243; Mature: 35243

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDALGDLSLDLLRAFEAAARHRSFTAAAVELGTTQPAISQQIKRLEEQLGTRLFDRIYR
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GIELTEVGSILFEQVTLGLQNIDAGLSAISTQQQHEVLQVATDFAFAAYWLMPRLHRFHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYPQVDVSLVTSERNHNMLRPDIDVAVLFGDGRFKQGESHWLFSEEVFPVCSPQLLKDCS
HCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHCC
LPLPAQALMEFPLLHLRGENSSNWFDWSGLFRALNISTPPAPGQLRFDNYTLLIQAAIGG
CCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHEEECCCCCCCCEEEECCEEEEEEEECCC
QGVAIGWRHLVDNLLTQGLLCRPIAETTLSRLGYYVVLPQRKRRGALIQTFVDWLMAEQA
CCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHC
SSAESLAGLPLPSIAV
CCHHHHCCCCCCCCCC
>Mature Secondary Structure
MYDALGDLSLDLLRAFEAAARHRSFTAAAVELGTTQPAISQQIKRLEEQLGTRLFDRIYR
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GIELTEVGSILFEQVTLGLQNIDAGLSAISTQQQHEVLQVATDFAFAAYWLMPRLHRFHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYPQVDVSLVTSERNHNMLRPDIDVAVLFGDGRFKQGESHWLFSEEVFPVCSPQLLKDCS
HCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHCC
LPLPAQALMEFPLLHLRGENSSNWFDWSGLFRALNISTPPAPGQLRFDNYTLLIQAAIGG
CCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHEEECCCCCCCCEEEECCEEEEEEEECCC
QGVAIGWRHLVDNLLTQGLLCRPIAETTLSRLGYYVVLPQRKRRGALIQTFVDWLMAEQA
CCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHC
SSAESLAGLPLPSIAV
CCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2788410 [H]