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The map label for this gene is smf [H]

Identifier: 77456247

GI number: 77456247

Start: 21282

End: 22382

Strand: Direct

Name: smf [H]

Synonym: Pfl01_0019

Alternate gene names: 77456247

Gene position: 21282-22382 (Clockwise)

Preceding gene: 77456246

Following gene: 77456248

Centisome position: 0.33

GC content: 65.3

Gene sequence:

>1101_bases
ATGATGATGCCTGTCTCTGCGTCGGTTTCCCCGGCGGAACTGGAAGCGCGCCTGCGCCTGCACCGCTTGCCGGAAGTCGG
TCCCAAACGTTTTGCCAAGCTGCTCGAAGCCTTCGGCTCTGCCTCCAAGGCAATCAGCGCGCCGGCCAGCGCCTGGCGCT
CGCTGGGTTTGCCGGCGGCTTGTGCCGAGGCCCGGCGCTGTCCTGAAGTTCGCGACGGCGCCAGCCACGCATTGCGCTGG
CTAGAGCGTCCGGATCAACATTTGCTGATGTGGGATCACCCGGATTATCCGGCCCTGCTGGCGCAGATTCCCGATCCACC
GCCCCTGCTGTTTGTCGCTGGCGACCCACGGATTCTGGAAAAACCGCAACTGGCGGTGATCGGCAGTCGCCGCGCTTCCC
GCCCGGGCATGGACACTGCCGCTGCGTTTTCCCGCAGTCTGGCCGGGGCCGGATTTGTCATCACCAGTGGGCTGGCGCTG
GGCATCGATGCGGCGGCGCATCAAGCTGCTGTCGACGTCGGCGGGCAAACGGTCGGCGTGCTCGGTACCGGTCTGGAAAA
CTTTTATCCACAGCGCAATCGGCGGTTGGCGGACGTGATGATTGCGTCCGGCAGTGCGGTGGTTTCGGAATTTCCTCTGG
ACACCGGGCCGACGGCCAGCAACTTCCCAAGACGCAATCGGATCATCAGCGGTTTGTCCCTCGGCGTGCTGGTGGTCGAG
GCCAGCGTGGCCAGCGGTTCGTTGATCACCGCGCGGCTGGCGGCGGAACAGGGTCGCGAGGTGTACGCCATTCCGGGTTC
GATCCACCACCCCGGCGCCCGGGGCTGTCATCAACTGATCCGTGACGGCGCCGTGCTGGTGGAAACCATCGAGCACATTC
TCGAAGCCCTGCGCGGCTGGCAACATCTGCCGGTATCCACAGCCGCAGCGAATCCGGACAACCCGTTGTTGCGTCTATTG
CACGCCGCGCCGCACACCAGCGAAGGGCTGGCCGACAGCAGTGGCTGGGCGTTACCCAAAGTCCTGGCGGCATTGACCGA
ACTGGAGATGGATGGCCGCGCGGTGTGCGAAAACGGCCGCTGGTTTGCGCGCGTGAGCTAG

Upstream 100 bases:

>100_bases
GACAAGGTGCGAAATCCTTAGCTGGCTTCACAAGTTACCAACAGAGTTATCCACAGCTTGTTCCGAAAGGTTCGGGCCTT
ATAACGATCAAGGATGATCC

Downstream 100 bases:

>100_bases
GTTTTACATTGAAGATCGGTAAACTGCGCGAAACCTGTTTGCGGAGAGTTTTTCATGGTCAACAGTTGGCGTGTGCAACA
AGCCGCACGAGAGATTCGCG

Product: SMF protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MMMPVSASVSPAELEARLRLHRLPEVGPKRFAKLLEAFGSASKAISAPASAWRSLGLPAACAEARRCPEVRDGASHALRW
LERPDQHLLMWDHPDYPALLAQIPDPPPLLFVAGDPRILEKPQLAVIGSRRASRPGMDTAAAFSRSLAGAGFVITSGLAL
GIDAAAHQAAVDVGGQTVGVLGTGLENFYPQRNRRLADVMIASGSAVVSEFPLDTGPTASNFPRRNRIISGLSLGVLVVE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAVLVETIEHILEALRGWQHLPVSTAAANPDNPLLRLL
HAAPHTSEGLADSSGWALPKVLAALTELEMDGRAVCENGRWFARVS

Sequences:

>Translated_366_residues
MMMPVSASVSPAELEARLRLHRLPEVGPKRFAKLLEAFGSASKAISAPASAWRSLGLPAACAEARRCPEVRDGASHALRW
LERPDQHLLMWDHPDYPALLAQIPDPPPLLFVAGDPRILEKPQLAVIGSRRASRPGMDTAAAFSRSLAGAGFVITSGLAL
GIDAAAHQAAVDVGGQTVGVLGTGLENFYPQRNRRLADVMIASGSAVVSEFPLDTGPTASNFPRRNRIISGLSLGVLVVE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAVLVETIEHILEALRGWQHLPVSTAAANPDNPLLRLL
HAAPHTSEGLADSSGWALPKVLAALTELEMDGRAVCENGRWFARVS
>Mature_366_residues
MMMPVSASVSPAELEARLRLHRLPEVGPKRFAKLLEAFGSASKAISAPASAWRSLGLPAACAEARRCPEVRDGASHALRW
LERPDQHLLMWDHPDYPALLAQIPDPPPLLFVAGDPRILEKPQLAVIGSRRASRPGMDTAAAFSRSLAGAGFVITSGLAL
GIDAAAHQAAVDVGGQTVGVLGTGLENFYPQRNRRLADVMIASGSAVVSEFPLDTGPTASNFPRRNRIISGLSLGVLVVE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAVLVETIEHILEALRGWQHLPVSTAAANPDNPLLRLL
HAAPHTSEGLADSSGWALPKVLAALTELEMDGRAVCENGRWFARVS

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=293, Percent_Identity=47.098976109215, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 38774; Mature: 38774

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMPVSASVSPAELEARLRLHRLPEVGPKRFAKLLEAFGSASKAISAPASAWRSLGLPAA
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHCCCCHH
CAEARRCPEVRDGASHALRWLERPDQHLLMWDHPDYPALLAQIPDPPPLLFVAGDPRILE
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCEEEECCCCCCCC
KPQLAVIGSRRASRPGMDTAAAFSRSLAGAGFVITSGLALGIDAAAHQAAVDVGGQTVGV
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEH
LGTGLENFYPQRNRRLADVMIASGSAVVSEFPLDTGPTASNFPRRNRIISGLSLGVLVVE
HHCCHHHHCCCCCCEEEHHEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAVLVETIEHILEALRGW
ECCCCCCHHEEHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC
QHLPVSTAAANPDNPLLRLLHAAPHTSEGLADSSGWALPKVLAALTELEMDGRAVCENGR
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCC
WFARVS
EEEEEC
>Mature Secondary Structure
MMMPVSASVSPAELEARLRLHRLPEVGPKRFAKLLEAFGSASKAISAPASAWRSLGLPAA
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHCHHHHHHHCCCCHH
CAEARRCPEVRDGASHALRWLERPDQHLLMWDHPDYPALLAQIPDPPPLLFVAGDPRILE
HHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCEEEECCCCCCCC
KPQLAVIGSRRASRPGMDTAAAFSRSLAGAGFVITSGLALGIDAAAHQAAVDVGGQTVGV
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCHHHCCHHHHHHHHHHCCCCEEEH
LGTGLENFYPQRNRRLADVMIASGSAVVSEFPLDTGPTASNFPRRNRIISGLSLGVLVVE
HHCCHHHHCCCCCCEEEHHEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEEE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRDGAVLVETIEHILEALRGW
ECCCCCCHHEEHHHHHCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC
QHLPVSTAAANPDNPLLRLLHAAPHTSEGLADSSGWALPKVLAALTELEMDGRAVCENGR
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCC
WFARVS
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]