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The map label for this gene is dnaK [H]

Identifier: 76819769

GI number: 76819769

Start: 71798

End: 73645

Strand: Direct

Name: dnaK [H]

Synonym: BURPS1710b_A0045

Alternate gene names: 76819769

Gene position: 71798-73645 (Clockwise)

Preceding gene: 76818106

Following gene: 76818939

Centisome position: 2.26

GC content: 72.94

Gene sequence:

>1848_bases
ATGAGCGACGCGCGTTATTCGATCGGCATCGATCTGGGCACCACCCACTGCGCGCTGTCGTACGTGGATCTCGCGGCGAG
CGACGGCGAGAAGACGAGCCTTCGCGTGCTGCCGATCGCGCAGCTCACCGCGCCGGGCGCAATCGACGCGCCGAATCTGC
TGCCGTCGTTTCTCTATCTGCCGCATCCGAGCGAGCTCGCGCCGGGCGAGCTTGCGCTGCCGTGGGCGGCCGAGCGCGAC
TTCGCGGTCGGCGAGCTCGCGCGCAGCCGCGGCGCGGGCACGCCGATCCGCCTCGTGTCGAGCGCGAAGAGCTGGCTGTG
CCATCCGGGCGTCGATCGCCGCGCCGGCATCCTGCCGAGCGACGCGCCGCCGGAAGTCGCGCGCGTGTCGCCGCTCGACG
CGTCGGTGCGCTATCTGTCCCATTTGCGCGAGGCGTGGAACCACGCGCATCCGGACGCGCCGTTCGATCGGCAGGACGTC
ACCGTCACGATTCCCGCGTCGTTCGATCCGGCCGCGCGCGAGCTGACGGCCGAGGCCGCGCAGGCGGCCGGCTACGCGCA
TATGACGCTGCTCGAGGAGCCGCAGGCGGCGCTCTACAGCTGGATCGAGAAAAGCGGCGGCGCGTGGCGCAAGGACGTCC
GGGTCGGCGACATCATCCTCGTCGTCGACGTCGGCGGCGGCACGACCGACCTGTCGCTGATCGCGGTCGTCGAGCGCGAC
GGCAACCTCGAGCTGCATCGCATCGCGGTCGGCGAGCATATCCTGCTCGGCGGCGACAACATGGATCTCGCGCTCGCGCA
CGTGGTCGCGCGCAAGCTCGCCGCGCAAGGCACGCAGGCCGACCCCTGGCAATTGCGCGCGCTCACCTACGCGTGCCGCG
GCGCGAAGGAGACGCTGCTCGGCGATCCGTCGGCGCAGGCCGTGCCGCTCGTCGTGCCGAGCCGCGGCGCGAAGCTGATC
GGCGGCTCGATCCGCACCGAGCTCACGCGCGCCGAGCTGACGCAGACGATCCTCGAAGGCTTCTTCCCGGCCGTCGACGC
GTCGGCGCGGCCGGTGAGCCGCACGCGCGCGGGCCTCATGCAGCTCGGCCTGCCGTACGCGCAGGACGCCGGCATCACGC
GCCATCTCGCCGCGTTCCTCGGCCGGCAGGCCGGAGCGCTCGCGGAGCTCGACGGGCTGCGGCACGCGCATGACGCGTCG
GCGAGCTTCCTGCATCCGAGCGCGGTGCTCTTCAACGGCGGCGTGTTCAAGTCGCCGCTGCTCGTCGAGCGCATGCTCGA
CACGCTCAACGGCTGGCTCGCGGCCGAGGGCGCGGCGCCCGCGCGTCTGCTCGGCGGCGCCGATCTCGATCTCGCGGTCG
CGCGCGGCGCGGCCTACTACGGCTACGTGAAGCGCGGCCGCGGCGTGCGCATTCGCGGCGGCACGGCGCGCGCGTATTAC
ATCGCGGTCGAATCGGCGATGCCGGCCGTGCCCGGCCTCGAGCCGCCGATCCAGGCGCTGTGCGTCGCGCCGTTCGGGAT
GGAGGAAGGCACCGACGCCGCGCTGCCGCCGCAGGAGTTCGGCCTCGTCGTCGGCGAGCCCGTCCGCTTCCGCTTCTTCG
GTTCGTCGGTGCGCCGGCAGGACCAGGTCGGCACGCTGCTCGACTTCTGGTCGCCCGACGAGCTGCAGGAGCTCGACGCG
ATCGAGGCGACGCTGCCGACCGCGGGCCGCACGCCGGGCGAGGTCGTGCCGGTGAGGCTGCACGCGCGCGTGACGGAGGC
CGGCACGCTCGAGCTCGAAGCCGTGCAAGGCGGCACGCACGAGCGCTGGAAGGTGGAGTTCGACGTGCGCGGCGGCGCGA
ACGCGTAA

Upstream 100 bases:

>100_bases
GCAGCATCAGCCATCGCGGCTGGCGCGTCGACGCCGTCCGGCTGCCGAAGCTGAACCCGGGCCACGACGCGTCGATCATC
GCCCCGGCCGAGGTGGCGCT

Downstream 100 bases:

>100_bases
CCGATGAAACGATTCATCGTCGGCATCGATCTCGGGACGAGCAATACCGTCGTGGCGTACGTCGAGGCGGGCGGCGACGC
GATCCGCGTGTTCGACGTCG

Product: heat shock protein 70

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSDARYSIGIDLGTTHCALSYVDLAASDGEKTSLRVLPIAQLTAPGAIDAPNLLPSFLYLPHPSELAPGELALPWAAERD
FAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAGILPSDAPPEVARVSPLDASVRYLSHLREAWNHAHPDAPFDRQDV
TVTIPASFDPAARELTAEAAQAAGYAHMTLLEEPQAALYSWIEKSGGAWRKDVRVGDIILVVDVGGGTTDLSLIAVVERD
GNLELHRIAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKETLLGDPSAQAVPLVVPSRGAKLI
GGSIRTELTRAELTQTILEGFFPAVDASARPVSRTRAGLMQLGLPYAQDAGITRHLAAFLGRQAGALAELDGLRHAHDAS
ASFLHPSAVLFNGGVFKSPLLVERMLDTLNGWLAAEGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
IAVESAMPAVPGLEPPIQALCVAPFGMEEGTDAALPPQEFGLVVGEPVRFRFFGSSVRRQDQVGTLLDFWSPDELQELDA
IEATLPTAGRTPGEVVPVRLHARVTEAGTLELEAVQGGTHERWKVEFDVRGGANA

Sequences:

>Translated_615_residues
MSDARYSIGIDLGTTHCALSYVDLAASDGEKTSLRVLPIAQLTAPGAIDAPNLLPSFLYLPHPSELAPGELALPWAAERD
FAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAGILPSDAPPEVARVSPLDASVRYLSHLREAWNHAHPDAPFDRQDV
TVTIPASFDPAARELTAEAAQAAGYAHMTLLEEPQAALYSWIEKSGGAWRKDVRVGDIILVVDVGGGTTDLSLIAVVERD
GNLELHRIAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKETLLGDPSAQAVPLVVPSRGAKLI
GGSIRTELTRAELTQTILEGFFPAVDASARPVSRTRAGLMQLGLPYAQDAGITRHLAAFLGRQAGALAELDGLRHAHDAS
ASFLHPSAVLFNGGVFKSPLLVERMLDTLNGWLAAEGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
IAVESAMPAVPGLEPPIQALCVAPFGMEEGTDAALPPQEFGLVVGEPVRFRFFGSSVRRQDQVGTLLDFWSPDELQELDA
IEATLPTAGRTPGEVVPVRLHARVTEAGTLELEAVQGGTHERWKVEFDVRGGANA
>Mature_614_residues
SDARYSIGIDLGTTHCALSYVDLAASDGEKTSLRVLPIAQLTAPGAIDAPNLLPSFLYLPHPSELAPGELALPWAAERDF
AVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAGILPSDAPPEVARVSPLDASVRYLSHLREAWNHAHPDAPFDRQDVT
VTIPASFDPAARELTAEAAQAAGYAHMTLLEEPQAALYSWIEKSGGAWRKDVRVGDIILVVDVGGGTTDLSLIAVVERDG
NLELHRIAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKETLLGDPSAQAVPLVVPSRGAKLIG
GSIRTELTRAELTQTILEGFFPAVDASARPVSRTRAGLMQLGLPYAQDAGITRHLAAFLGRQAGALAELDGLRHAHDASA
SFLHPSAVLFNGGVFKSPLLVERMLDTLNGWLAAEGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYYI
AVESAMPAVPGLEPPIQALCVAPFGMEEGTDAALPPQEFGLVVGEPVRFRFFGSSVRRQDQVGTLLDFWSPDELQELDAI
EATLPTAGRTPGEVVPVRLHARVTEAGTLELEAVQGGTHERWKVEFDVRGGANA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=408, Percent_Identity=24.7549019607843, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17541098, Length=202, Percent_Identity=29.2079207920792, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 65294; Mature: 65163

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDARYSIGIDLGTTHCALSYVDLAASDGEKTSLRVLPIAQLTAPGAIDAPNLLPSFLYL
CCCCEEEEEEECCCHHHHHEEHEEECCCCCCCEEEEEEEHHCCCCCCCCCCHHHHHHEEC
PHPSELAPGELALPWAAERDFAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAGILPS
CCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
DAPPEVARVSPLDASVRYLSHLREAWNHAHPDAPFDRQDVTVTIPASFDPAARELTAEAA
CCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
QAAGYAHMTLLEEPQAALYSWIEKSGGAWRKDVRVGDIILVVDVGGGTTDLSLIAVVERD
HHCCCEEEEECCCHHHHHHHHHHHCCCCEECCCEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRIAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHC
GDPSAQAVPLVVPSRGAKLIGGSIRTELTRAELTQTILEGFFPAVDASARPVSRTRAGLM
CCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QLGLPYAQDAGITRHLAAFLGRQAGALAELDGLRHAHDASASFLHPSAVLFNGGVFKSPL
HHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCHH
LVERMLDTLNGWLAAEGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHCCEEEECCCCCHHHCCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCCEEEE
IAVESAMPAVPGLEPPIQALCVAPFGMEEGTDAALPPQEFGLVVGEPVRFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEEECCHHHHH
DQVGTLLDFWSPDELQELDAIEATLPTAGRTPGEVVPVRLHARVTEAGTLELEAVQGGTH
HHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCC
ERWKVEFDVRGGANA
CEEEEEEEECCCCCC
>Mature Secondary Structure 
SDARYSIGIDLGTTHCALSYVDLAASDGEKTSLRVLPIAQLTAPGAIDAPNLLPSFLYL
CCCEEEEEEECCCHHHHHEEHEEECCCCCCCEEEEEEEHHCCCCCCCCCCHHHHHHEEC
PHPSELAPGELALPWAAERDFAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAGILPS
CCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
DAPPEVARVSPLDASVRYLSHLREAWNHAHPDAPFDRQDVTVTIPASFDPAARELTAEAA
CCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
QAAGYAHMTLLEEPQAALYSWIEKSGGAWRKDVRVGDIILVVDVGGGTTDLSLIAVVERD
HHCCCEEEEECCCHHHHHHHHHHHCCCCEECCCEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRIAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHC
GDPSAQAVPLVVPSRGAKLIGGSIRTELTRAELTQTILEGFFPAVDASARPVSRTRAGLM
CCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
QLGLPYAQDAGITRHLAAFLGRQAGALAELDGLRHAHDASASFLHPSAVLFNGGVFKSPL
HHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCHH
LVERMLDTLNGWLAAEGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHCCEEEECCCCCHHHCCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCCEEEE
IAVESAMPAVPGLEPPIQALCVAPFGMEEGTDAALPPQEFGLVVGEPVRFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEEECCHHHHH
DQVGTLLDFWSPDELQELDAIEATLPTAGRTPGEVVPVRLHARVTEAGTLELEAVQGGTH
HHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCC
ERWKVEFDVRGGANA
CEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]