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The map label for this gene is iscS-2 [H]

Identifier: 76818462

GI number: 76818462

Start: 1535274

End: 1536518

Strand: Direct

Name: iscS-2 [H]

Synonym: BURPS1710b_A1225

Alternate gene names: 76818462

Gene position: 1535274-1536518 (Clockwise)

Preceding gene: 76817847

Following gene: 76817825

Centisome position: 48.25

GC content: 65.7

Gene sequence:

>1245_bases
ATGCGATCCTGTCGATCGCGGCCGTCACGTTCCTGTCAGGCGCCGTGGTGGCGATCGTCATGCACGCGCGCCACTGACGT
ATTCCGAAGCACGAAGCGAACATTTCTCACCCAGATCGACGACATGACGCATATCTACCTCGACTACAACGCAAGCACCC
CCATCGACCCGGCGGTCGCGGATGCGATGCGCCCGCTGCTGCCTGAAGCCTTCGGCAATCCGTCCAGCGGACATTGGGCA
AGCGTGCCGGCAAAGGTTGCACTGGATCGGGCGCGCGCCCAGGTCGCGACGCTGATCGGCGCCGCACCGGACGAAATTGT
CTTCACGAGCGGCGGCAGTGAAGCGAACAACCTTGCGCTGAAAGGAACTGTACTCGCATCGTCGAACGCAGGCGGCCACG
TGATCACCCAGTCGACCGAACATCCGGCAATCCTGAAGCCGCTGCAATTCCTTGAGCGACTGGGCACTAAAGTCACCTGC
GTTCCCGTGGACGGCAACGGCCGCGTCGATCCCGACGCCATCCGTCGCGCAATCACGCCGCGCACCACGCTCATCACGGT
CATGCATGCGAACAACGAAACCGGCACCATTCAGCCGATCGAGGATATCGGCACGATCGCCCGCGAGCACGGCATCCGGT
TTCACACGGACGCGGCCCAGTCGATCGGCAAGATCCCGGCCGACGTCGATGCGCTCGGCGTCGATCTGCTGTCGATCGCC
GGTCACAAGCTCTACGCGCCGAAGGGCATCGGCGCGCTCTACGTTCGAAAGGGAGTCGGGCTCGAGCCGCTGATTCACGG
CGCAGGCCATGAAAATGGCCGGCGTGCGGGGACGGAATCCGCACTCCTCGCGGCAGGGTTCGGCGAGGCCTGTGCGCTCG
CGCGCGATCTCGCGGCGATGGACGGCATTCGCGCGTTGCGCGACCGGCTGTGGCACGCGTTGCAGGATCGCTTCGACGAT
CGCGTTCGCCTGAACGGACATCTCGAGTACCGCTTGCCGAACACGGTGAACGTGTCGTTCGTCGGCCGCGTCGGCGCTGA
CGTGCTTGCCGCGCTCGACGGCGTCGCCGCGTCCACCGGTTCGGCCTGCCACGCCGGACGCATGACGCTGTCGCCGGTGC
TGGCCGCGATGGGCATCGCCGAGCAGGTCGGGATGGGGGCCATCCGATTCAGCCTCGGCCGGCACACGACAGCCGACGAG
ATCGATACCGTCGTCGCGAAATTGAGCGCGATCGCCCGTGGCTGA

Upstream 100 bases:

>100_bases
CGTGCCCGGGCGCTGAGCGTGTACCGGTTCTGGCGTGACCTCGGCTATGCGATCGGCGCGCTGTCGGCGGGTCTCATCGC
GGACCGCTTCGGCTTCGCCG

Downstream 100 bases:

>100_bases
AATCGGTGCTATGCGACGATGCCCGTGTAGTCGAATGAAGCGCGAGCAGCGTGATCCGCGGTGGCTTCGCCTGATCGCGG
GTGCCCCGACCGTTCGGGAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRSCRSRPSRSCQAPWWRSSCTRATDVFRSTKRTFLTQIDDMTHIYLDYNASTPIDPAVADAMRPLLPEAFGNPSSGHWA
SVPAKVALDRARAQVATLIGAAPDEIVFTSGGSEANNLALKGTVLASSNAGGHVITQSTEHPAILKPLQFLERLGTKVTC
VPVDGNGRVDPDAIRRAITPRTTLITVMHANNETGTIQPIEDIGTIAREHGIRFHTDAAQSIGKIPADVDALGVDLLSIA
GHKLYAPKGIGALYVRKGVGLEPLIHGAGHENGRRAGTESALLAAGFGEACALARDLAAMDGIRALRDRLWHALQDRFDD
RVRLNGHLEYRLPNTVNVSFVGRVGADVLAALDGVAASTGSACHAGRMTLSPVLAAMGIAEQVGMGAIRFSLGRHTTADE
IDTVVAKLSAIARG

Sequences:

>Translated_414_residues
MRSCRSRPSRSCQAPWWRSSCTRATDVFRSTKRTFLTQIDDMTHIYLDYNASTPIDPAVADAMRPLLPEAFGNPSSGHWA
SVPAKVALDRARAQVATLIGAAPDEIVFTSGGSEANNLALKGTVLASSNAGGHVITQSTEHPAILKPLQFLERLGTKVTC
VPVDGNGRVDPDAIRRAITPRTTLITVMHANNETGTIQPIEDIGTIAREHGIRFHTDAAQSIGKIPADVDALGVDLLSIA
GHKLYAPKGIGALYVRKGVGLEPLIHGAGHENGRRAGTESALLAAGFGEACALARDLAAMDGIRALRDRLWHALQDRFDD
RVRLNGHLEYRLPNTVNVSFVGRVGADVLAALDGVAASTGSACHAGRMTLSPVLAAMGIAEQVGMGAIRFSLGRHTTADE
IDTVVAKLSAIARG
>Mature_414_residues
MRSCRSRPSRSCQAPWWRSSCTRATDVFRSTKRTFLTQIDDMTHIYLDYNASTPIDPAVADAMRPLLPEAFGNPSSGHWA
SVPAKVALDRARAQVATLIGAAPDEIVFTSGGSEANNLALKGTVLASSNAGGHVITQSTEHPAILKPLQFLERLGTKVTC
VPVDGNGRVDPDAIRRAITPRTTLITVMHANNETGTIQPIEDIGTIAREHGIRFHTDAAQSIGKIPADVDALGVDLLSIA
GHKLYAPKGIGALYVRKGVGLEPLIHGAGHENGRRAGTESALLAAGFGEACALARDLAAMDGIRALRDRLWHALQDRFDD
RVRLNGHLEYRLPNTVNVSFVGRVGADVLAALDGVAASTGSACHAGRMTLSPVLAAMGIAEQVGMGAIRFSLGRHTTADE
IDTVVAKLSAIARG

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=368, Percent_Identity=43.4782608695652, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=40.3940886699507, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI48994898, Length=368, Percent_Identity=45.6521739130435, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787970, Length=412, Percent_Identity=25.4854368932039, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789175, Length=379, Percent_Identity=27.4406332453826, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=367, Percent_Identity=43.3242506811989, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17533177, Length=315, Percent_Identity=36.8253968253968, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=44.0540540540541, Blast_Score=289, Evalue=5e-79,
Organism=Drosophila melanogaster, GI20129463, Length=369, Percent_Identity=44.7154471544715, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43873; Mature: 43873

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSCRSRPSRSCQAPWWRSSCTRATDVFRSTKRTFLTQIDDMTHIYLDYNASTPIDPAVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
DAMRPLLPEAFGNPSSGHWASVPAKVALDRARAQVATLIGAAPDEIVFTSGGSEANNLAL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEE
KGTVLASSNAGGHVITQSTEHPAILKPLQFLERLGTKVTCVPVDGNGRVDPDAIRRAITP
EEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCC
RTTLITVMHANNETGTIQPIEDIGTIAREHGIRFHTDAAQSIGKIPADVDALGVDLLSIA
CEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHHCCCCCCHHHHHHHHHHHC
GHKLYAPKGIGALYVRKGVGLEPLIHGAGHENGRRAGTESALLAAGFGEACALARDLAAM
CCEEECCCCCCEEEEECCCCCCHHEECCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHH
DGIRALRDRLWHALQDRFDDRVRLNGHLEYRLPNTVNVSFVGRVGADVLAALDGVAASTG
HHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCC
SACHAGRMTLSPVLAAMGIAEQVGMGAIRFSLGRHTTADEIDTVVAKLSAIARG
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSCRSRPSRSCQAPWWRSSCTRATDVFRSTKRTFLTQIDDMTHIYLDYNASTPIDPAVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
DAMRPLLPEAFGNPSSGHWASVPAKVALDRARAQVATLIGAAPDEIVFTSGGSEANNLAL
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEE
KGTVLASSNAGGHVITQSTEHPAILKPLQFLERLGTKVTCVPVDGNGRVDPDAIRRAITP
EEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCC
RTTLITVMHANNETGTIQPIEDIGTIAREHGIRFHTDAAQSIGKIPADVDALGVDLLSIA
CEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECHHHHHHCCCCCCHHHHHHHHHHHC
GHKLYAPKGIGALYVRKGVGLEPLIHGAGHENGRRAGTESALLAAGFGEACALARDLAAM
CCEEECCCCCCEEEEECCCCCCHHEECCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHH
DGIRALRDRLWHALQDRFDDRVRLNGHLEYRLPNTVNVSFVGRVGADVLAALDGVAASTG
HHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCC
SACHAGRMTLSPVLAAMGIAEQVGMGAIRFSLGRHTTADEIDTVVAKLSAIARG
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA