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The map label for this gene is shiA [H]

Identifier: 76817825

GI number: 76817825

Start: 1537859

End: 1539193

Strand: Direct

Name: shiA [H]

Synonym: BURPS1710b_A1226

Alternate gene names: 76817825

Gene position: 1537859-1539193 (Clockwise)

Preceding gene: 76818462

Following gene: 76818080

Centisome position: 48.33

GC content: 68.09

Gene sequence:

>1335_bases
ATGCCAACCCCGACGCCCGGCACGCCCCGCCCTCCCCGCACGCCCCAACCCGTGCGCGCCGCGCTGGCCGCCTTCATCGG
CACCACCGTCGAGTTCTACGATTACTACACGTACGCCACCGCCGCCGCGCTCGTGCTCGGCGACGTGTTCTTCCCGAGCG
ACAACCCTTTCGTCAGCACGATGGCATCGTTCGCCACGTTCTTCGTCGGCTTCGTCGCGCGCCCACTGAGCGGTGCGGTG
TTCGGCCATCTCGGCGACCGGATCGGCCGCAAGAAAATGCTCGTCCTGACGATGTTCCTGATGGGCGCCGCGACAACCGG
CATCGGCCTGCTGCCCGGCTACGCGACGATCGGCGTCTGGGCCCCCGTCCTGCTCGTGCTGCTGCGCATCCTCCAGGGCA
TCGCCGTCGGCGGCGAATGGGGCGGCGCGGTGCTGATGGCAAGCGAGCACGCGAGCGCCGGGCGCAGGACGCTCTTCGCG
TCGTTTCCGCAAATGGGCAGCCCCGCCGGGCTGATCCTCTCGCTGCTCGCGTTCCGCTACGTCACGTCGCTCGATCACGC
CCGCTTCGTCGCGTGGGGCTGGCGCCTGCCGTTTCTCGCAAGCTTCGTGCTGTTGCTGATCGGCGTCGCGGTACGGCTCG
GCGTCAACGAATCGCCGGAGTTCGAGCGCGTGCGCGACAGCCACGCGGTCGCCCGATTTCCGGTCGCCGAAGTGCTGCGC
ACCGCGTGGTATCCGATCGTGCTCGCCGCGGCCGCGACGACGATCGGCTCCGCCGGCTTCTTCTTCACGAACACGTTCAT
GATCTCGTACGTGACGACGTATCTCGGGATGTCGAAATCGTTCATCCTCGATTGCCTGTTCGCCGTCACGCTCATCCAGT
TGCTGTCGCAGCCCGTCTCGGCGCTGCTCGCGCAACGTTTCGGCGAAACGCGTTTCCTCGTCTGCGCGGCGCTGCTGTCG
ATGCTCACGCCGTATCCGATGTTCGTGCTCGTGCGCACGCACGATCCGGTTGCGATCGTCGCGGGCATCGCGTTCGCGGT
CGTCGCGCTCTCCGCCGTCTACGCGGTGATCGCCGGCTTCATGTCCGCCGCGTTCCCCACGCGCGTGCGCTATTCGGGCA
TCTCGATCGCCTACCAGCTATGCGCGCTAGTCGCGGGCGGCACCACGCCGCTCGTCGGCACGATGATCGCGCAGCGCTAT
CGCGGAGAATGGCTGCCGCTCGCGCTGTTCTTCTCGCTGCTGGCGTTCGTGTCGCTCGTCGGCATCGTGGGGCTCGGGCG
GTATCGCCGGAATCTGCACGCGGCCGCGCCGCGGGCATGGGCGGTGAATCCGTGA

Upstream 100 bases:

>100_bases
CAAAGCCAAAACGACCGACCGCCTCCCCGCCCGCCGCCCACGCCGCCCGATCATCCCGAAGCCCCGGCATCACGCTGCAT
CCCTACCCAAAGGACCGCCC

Downstream 100 bases:

>100_bases
GAGCGCGCGCCGCACGGCCGCGCGCACGCCGACTTCGCGCGACGCGCGCCGTGGCCGCTCGGCGCGTGCTCGCCCTTCGC
CCTTCGCCCTTCGCCCTTCG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPTPTPGTPRPPRTPQPVRAALAAFIGTTVEFYDYYTYATAAALVLGDVFFPSDNPFVSTMASFATFFVGFVARPLSGAV
FGHLGDRIGRKKMLVLTMFLMGAATTGIGLLPGYATIGVWAPVLLVLLRILQGIAVGGEWGGAVLMASEHASAGRRTLFA
SFPQMGSPAGLILSLLAFRYVTSLDHARFVAWGWRLPFLASFVLLLIGVAVRLGVNESPEFERVRDSHAVARFPVAEVLR
TAWYPIVLAAAATTIGSAGFFFTNTFMISYVTTYLGMSKSFILDCLFAVTLIQLLSQPVSALLAQRFGETRFLVCAALLS
MLTPYPMFVLVRTHDPVAIVAGIAFAVVALSAVYAVIAGFMSAAFPTRVRYSGISIAYQLCALVAGGTTPLVGTMIAQRY
RGEWLPLALFFSLLAFVSLVGIVGLGRYRRNLHAAAPRAWAVNP

Sequences:

>Translated_444_residues
MPTPTPGTPRPPRTPQPVRAALAAFIGTTVEFYDYYTYATAAALVLGDVFFPSDNPFVSTMASFATFFVGFVARPLSGAV
FGHLGDRIGRKKMLVLTMFLMGAATTGIGLLPGYATIGVWAPVLLVLLRILQGIAVGGEWGGAVLMASEHASAGRRTLFA
SFPQMGSPAGLILSLLAFRYVTSLDHARFVAWGWRLPFLASFVLLLIGVAVRLGVNESPEFERVRDSHAVARFPVAEVLR
TAWYPIVLAAAATTIGSAGFFFTNTFMISYVTTYLGMSKSFILDCLFAVTLIQLLSQPVSALLAQRFGETRFLVCAALLS
MLTPYPMFVLVRTHDPVAIVAGIAFAVVALSAVYAVIAGFMSAAFPTRVRYSGISIAYQLCALVAGGTTPLVGTMIAQRY
RGEWLPLALFFSLLAFVSLVGIVGLGRYRRNLHAAAPRAWAVNP
>Mature_443_residues
PTPTPGTPRPPRTPQPVRAALAAFIGTTVEFYDYYTYATAAALVLGDVFFPSDNPFVSTMASFATFFVGFVARPLSGAVF
GHLGDRIGRKKMLVLTMFLMGAATTGIGLLPGYATIGVWAPVLLVLLRILQGIAVGGEWGGAVLMASEHASAGRRTLFAS
FPQMGSPAGLILSLLAFRYVTSLDHARFVAWGWRLPFLASFVLLLIGVAVRLGVNESPEFERVRDSHAVARFPVAEVLRT
AWYPIVLAAAATTIGSAGFFFTNTFMISYVTTYLGMSKSFILDCLFAVTLIQLLSQPVSALLAQRFGETRFLVCAALLSM
LTPYPMFVLVRTHDPVAIVAGIAFAVVALSAVYAVIAGFMSAAFPTRVRYSGISIAYQLCALVAGGTTPLVGTMIAQRYR
GEWLPLALFFSLLAFVSLVGIVGLGRYRRNLHAAAPRAWAVNP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=412, Percent_Identity=38.5922330097087, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1790550, Length=432, Percent_Identity=31.712962962963, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1789941, Length=390, Percent_Identity=35.8974358974359, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788942, Length=387, Percent_Identity=28.6821705426357, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87082231, Length=501, Percent_Identity=23.3532934131737, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47660; Mature: 47529

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPTPGTPRPPRTPQPVRAALAAFIGTTVEFYDYYTYATAAALVLGDVFFPSDNPFVST
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MASFATFFVGFVARPLSGAVFGHLGDRIGRKKMLVLTMFLMGAATTGIGLLPGYATIGVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
APVLLVLLRILQGIAVGGEWGGAVLMASEHASAGRRTLFASFPQMGSPAGLILSLLAFRY
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
VTSLDHARFVAWGWRLPFLASFVLLLIGVAVRLGVNESPEFERVRDSHAVARFPVAEVLR
HHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHH
TAWYPIVLAAAATTIGSAGFFFTNTFMISYVTTYLGMSKSFILDCLFAVTLIQLLSQPVS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALLAQRFGETRFLVCAALLSMLTPYPMFVLVRTHDPVAIVAGIAFAVVALSAVYAVIAGF
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
MSAAFPTRVRYSGISIAYQLCALVAGGTTPLVGTMIAQRYRGEWLPLALFFSLLAFVSLV
HHHHCCCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GIVGLGRYRRNLHAAAPRAWAVNP
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PTPTPGTPRPPRTPQPVRAALAAFIGTTVEFYDYYTYATAAALVLGDVFFPSDNPFVST
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
MASFATFFVGFVARPLSGAVFGHLGDRIGRKKMLVLTMFLMGAATTGIGLLPGYATIGVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
APVLLVLLRILQGIAVGGEWGGAVLMASEHASAGRRTLFASFPQMGSPAGLILSLLAFRY
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
VTSLDHARFVAWGWRLPFLASFVLLLIGVAVRLGVNESPEFERVRDSHAVARFPVAEVLR
HHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHH
TAWYPIVLAAAATTIGSAGFFFTNTFMISYVTTYLGMSKSFILDCLFAVTLIQLLSQPVS
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ALLAQRFGETRFLVCAALLSMLTPYPMFVLVRTHDPVAIVAGIAFAVVALSAVYAVIAGF
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
MSAAFPTRVRYSGISIAYQLCALVAGGTTPLVGTMIAQRYRGEWLPLALFFSLLAFVSLV
HHHHCCCHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GIVGLGRYRRNLHAAAPRAWAVNP
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]