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The map label for this gene is nrdE [H]

Identifier: 76818060

GI number: 76818060

Start: 1733199

End: 1735118

Strand: Direct

Name: nrdE [H]

Synonym: BURPS1710b_A1422

Alternate gene names: 76818060

Gene position: 1733199-1735118 (Clockwise)

Preceding gene: 76817511

Following gene: 76819494

Centisome position: 54.47

GC content: 74.27

Gene sequence:

>1920_bases
TTGCCGCGCCGCTCGTCGTACCGCTCTTCGTACCGCTCGTCGTGCCGCCCGCCGCACCGCGCGGCTCGCGCATGCCGCTT
GCGGACGATCAACAAACGCACGCGCACCATTGCCCACGCCGATTCGTTCGTCCGCATGATCGGCGACATGGACGACCAAC
CGATCTGCGACGCCGTATTCGCCGACCGCTACGCGCTGCCCGGCGAGCATTCGCGCGCTCAGGCGTGCGCGCGGGTCGCG
CGTGCGCTCGCGCTCGCGGAGCCGATGGCGACCCGTTCGGGCGCGGCGCGGCGCTTCTACCGGAACCTGCTGAACGGCGC
GTTCGGCGCGGGCCGCATCATGGCGCGCGCGGGCGCCGCGCCGGATCAGACGATGGCGAGCTGCTTCGTGCATCCGATCC
GCGCCCCCGCCGCGCTCACGCGCTTTCATCCGAACCTCGACGAGGCGCTCGACGAAGCGCGCCTCGCGCTCGCGATGGGC
GCCGACATCGGCTACGACTTCTCGGCCATCCCGCCCGCCGGCGCGCGGCCCGACGCGGACCATCCCGATTCGCCCGGCGT
GTGCGCGGCGCTCGACCGGTTCGACCGGATCGGCACGCAGGCGGGCGAGCGCGACGGCCGCCGCCGCGCGCAGCTCGCGG
TGCTGCGCTGCGATCACCCGGATCTGCTCGCGTTCGCCGCCGCGAAGCACCGGCATGCGGGCAGGCATGCGCGCTGGACG
ACGCTCGAGCTCGCGGTGGCCGCCACCGACGCATTCATGCAGGCGGTCGAGCAGGATCTGCCGTGGACGCTGCGGCACAC
GGCGCCGCCGCGCGACGCGCCGGGCGGCGCGCTGCCCGGCGCGGACGGCGCATGGACGTACGCGAGCGTGCCCGCGCGCC
ACGTGTGGCGCGAGATCGTGTCGGCCGCGCGCGACGGCGCGGGGCCCGGCCTCGTATTCGTCGATGCGATCGACGCCGCC
GATCCGCTGCGCGGCCGCGAGCGGATCGACGCGACGAGCCCGTGCGGCGCGCAGCCGCTGCCGCCCTACGGCAGCGCGAT
GCTCGGCGCGATCGATCTGTCGCGCTTCGTGCGCAATCCGTTCGGCGCCGGCGGCGAGCCGCGCTTCGATTTCGCCGCGT
TCGACACGGCCGTGCGCATCGAGGTGCGCCTGCTCGACAACGCGCTCGACGTCACGCGCTGGCCGCTCGCCGCGCATGCG
CGCGAGTCGTATCAGAAGCGGCGGATCGGCGTCGGCGTGACGGGGCTCGCGGACATGCTCGCGATGATGCGGCTGCGCTA
CGATTCGCCGGCCGCGCGCGAGATGGCGCGCTACATCGCGTCCGACCTGCGCCACCATGCGTACGCGGCGTCGGCCGAGC
TCGCCGCCGAGCGCGGCGCGTATCCGCTGTGCGACCGCCACGCGCATCTCGACGCGCTGCGCGCGGGGCCGCCGCTGCCG
CACGCGGTCTGCCACGCGATCGAGCGCGACGGGCTGCGCCACAGTCATCTGACGTCGTTCGCGCCCGTCATCGGCGTGAG
CCTCGCGTTCGGCGATCACTGCTCGCCCGGCATCGAGCCGGCCCGCGCGTGGATCGAGCACCGCCCGGTGCGCGCGGGCG
CCGCCGGCGCGCCGGGCATGCGCGCGGAAAACCACGCGCACCGGCTGTTCCGGTCGCTGCGCGGCGAGCGCGCCGCGCTG
CCGGACTACTTCGCGACCGATGCGGACGTCGCGCCGGGCGAGCGCCTCGCGATGCGCGTGGCGCTGCAGCCGTATGTCGA
CGCGGGAATCGACAATACGCTGACCCTCGCGAACCATTACTCGCTCGAAGAGGTCAACGCATTGCTGTTCGCCGCGTGGC
GCGCGAAGCTGAAGAGCGTGGCGATACGGCGCGCGGATCTCGCGCGCGACGCGCAGCCGAGCGACGACGACGGCGTTTAG

Upstream 100 bases:

>100_bases
CCGCTCGCCGCGCTGCCCGTCATGCCGCTCGCCGCGCCGCTCGCCGCACCACCCGCCATGTCGCTCGCCGTGCCGCTCAC
CGCACCACCCGCCATGTCGC

Downstream 100 bases:

>100_bases
GCAGGCCCGCGGCCCACGCGACGCGCGCGGGCGCTTGACCCACGTCAAGCCGGCGCCCGGCGCGATCGGGTCGAATGACG
ACGGGCGCCGTCGCGGCGCG

Product: ribonucleotide reductase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MPRRSSYRSSYRSSCRPPHRAARACRLRTINKRTRTIAHADSFVRMIGDMDDQPICDAVFADRYALPGEHSRAQACARVA
RALALAEPMATRSGAARRFYRNLLNGAFGAGRIMARAGAAPDQTMASCFVHPIRAPAALTRFHPNLDEALDEARLALAMG
ADIGYDFSAIPPAGARPDADHPDSPGVCAALDRFDRIGTQAGERDGRRRAQLAVLRCDHPDLLAFAAAKHRHAGRHARWT
TLELAVAATDAFMQAVEQDLPWTLRHTAPPRDAPGGALPGADGAWTYASVPARHVWREIVSAARDGAGPGLVFVDAIDAA
DPLRGRERIDATSPCGAQPLPPYGSAMLGAIDLSRFVRNPFGAGGEPRFDFAAFDTAVRIEVRLLDNALDVTRWPLAAHA
RESYQKRRIGVGVTGLADMLAMMRLRYDSPAAREMARYIASDLRHHAYAASAELAAERGAYPLCDRHAHLDALRAGPPLP
HAVCHAIERDGLRHSHLTSFAPVIGVSLAFGDHCSPGIEPARAWIEHRPVRAGAAGAPGMRAENHAHRLFRSLRGERAAL
PDYFATDADVAPGERLAMRVALQPYVDAGIDNTLTLANHYSLEEVNALLFAAWRAKLKSVAIRRADLARDAQPSDDDGV

Sequences:

>Translated_639_residues
MPRRSSYRSSYRSSCRPPHRAARACRLRTINKRTRTIAHADSFVRMIGDMDDQPICDAVFADRYALPGEHSRAQACARVA
RALALAEPMATRSGAARRFYRNLLNGAFGAGRIMARAGAAPDQTMASCFVHPIRAPAALTRFHPNLDEALDEARLALAMG
ADIGYDFSAIPPAGARPDADHPDSPGVCAALDRFDRIGTQAGERDGRRRAQLAVLRCDHPDLLAFAAAKHRHAGRHARWT
TLELAVAATDAFMQAVEQDLPWTLRHTAPPRDAPGGALPGADGAWTYASVPARHVWREIVSAARDGAGPGLVFVDAIDAA
DPLRGRERIDATSPCGAQPLPPYGSAMLGAIDLSRFVRNPFGAGGEPRFDFAAFDTAVRIEVRLLDNALDVTRWPLAAHA
RESYQKRRIGVGVTGLADMLAMMRLRYDSPAAREMARYIASDLRHHAYAASAELAAERGAYPLCDRHAHLDALRAGPPLP
HAVCHAIERDGLRHSHLTSFAPVIGVSLAFGDHCSPGIEPARAWIEHRPVRAGAAGAPGMRAENHAHRLFRSLRGERAAL
PDYFATDADVAPGERLAMRVALQPYVDAGIDNTLTLANHYSLEEVNALLFAAWRAKLKSVAIRRADLARDAQPSDDDGV
>Mature_638_residues
PRRSSYRSSYRSSCRPPHRAARACRLRTINKRTRTIAHADSFVRMIGDMDDQPICDAVFADRYALPGEHSRAQACARVAR
ALALAEPMATRSGAARRFYRNLLNGAFGAGRIMARAGAAPDQTMASCFVHPIRAPAALTRFHPNLDEALDEARLALAMGA
DIGYDFSAIPPAGARPDADHPDSPGVCAALDRFDRIGTQAGERDGRRRAQLAVLRCDHPDLLAFAAAKHRHAGRHARWTT
LELAVAATDAFMQAVEQDLPWTLRHTAPPRDAPGGALPGADGAWTYASVPARHVWREIVSAARDGAGPGLVFVDAIDAAD
PLRGRERIDATSPCGAQPLPPYGSAMLGAIDLSRFVRNPFGAGGEPRFDFAAFDTAVRIEVRLLDNALDVTRWPLAAHAR
ESYQKRRIGVGVTGLADMLAMMRLRYDSPAAREMARYIASDLRHHAYAASAELAAERGAYPLCDRHAHLDALRAGPPLPH
AVCHAIERDGLRHSHLTSFAPVIGVSLAFGDHCSPGIEPARAWIEHRPVRAGAAGAPGMRAENHAHRLFRSLRGERAALP
DYFATDADVAPGERLAMRVALQPYVDAGIDNTLTLANHYSLEEVNALLFAAWRAKLKSVAIRRADLARDAQPSDDDGV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=431, Percent_Identity=22.5058004640371, Blast_Score=100, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17555242, Length=544, Percent_Identity=22.4264705882353, Blast_Score=105, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322123, Length=478, Percent_Identity=23.0125523012552, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320914, Length=357, Percent_Identity=23.249299719888, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17352481, Length=433, Percent_Identity=22.8637413394919, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013344
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 69274; Mature: 69143

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRSSYRSSYRSSCRPPHRAARACRLRTINKRTRTIAHADSFVRMIGDMDDQPICDAVF
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ADRYALPGEHSRAQACARVARALALAEPMATRSGAARRFYRNLLNGAFGAGRIMARAGAA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
PDQTMASCFVHPIRAPAALTRFHPNLDEALDEARLALAMGADIGYDFSAIPPAGARPDAD
CHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HPDSPGVCAALDRFDRIGTQAGERDGRRRAQLAVLRCDHPDLLAFAAAKHRHAGRHARWT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCEE
TLELAVAATDAFMQAVEQDLPWTLRHTAPPRDAPGGALPGADGAWTYASVPARHVWREIV
EEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHH
SAARDGAGPGLVFVDAIDAADPLRGRERIDATSPCGAQPLPPYGSAMLGAIDLSRFVRNP
HHHCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGAGGEPRFDFAAFDTAVRIEVRLLDNALDVTRWPLAAHARESYQKRRIGVGVTGLADML
CCCCCCCCCCHHHHCCEEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
AMMRLRYDSPAAREMARYIASDLRHHAYAASAELAAERGAYPLCDRHAHLDALRAGPPLP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCH
HAVCHAIERDGLRHSHLTSFAPVIGVSLAFGDHCSPGIEPARAWIEHRPVRAGAAGAPGM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
RAENHAHRLFRSLRGERAALPDYFATDADVAPGERLAMRVALQPYVDAGIDNTLTLANHY
CCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCC
SLEEVNALLFAAWRAKLKSVAIRRADLARDAQPSDDDGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PRRSSYRSSYRSSCRPPHRAARACRLRTINKRTRTIAHADSFVRMIGDMDDQPICDAVF
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ADRYALPGEHSRAQACARVARALALAEPMATRSGAARRFYRNLLNGAFGAGRIMARAGAA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
PDQTMASCFVHPIRAPAALTRFHPNLDEALDEARLALAMGADIGYDFSAIPPAGARPDAD
CHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HPDSPGVCAALDRFDRIGTQAGERDGRRRAQLAVLRCDHPDLLAFAAAKHRHAGRHARWT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCEE
TLELAVAATDAFMQAVEQDLPWTLRHTAPPRDAPGGALPGADGAWTYASVPARHVWREIV
EEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHH
SAARDGAGPGLVFVDAIDAADPLRGRERIDATSPCGAQPLPPYGSAMLGAIDLSRFVRNP
HHHCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGAGGEPRFDFAAFDTAVRIEVRLLDNALDVTRWPLAAHARESYQKRRIGVGVTGLADML
CCCCCCCCCCHHHHCCEEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
AMMRLRYDSPAAREMARYIASDLRHHAYAASAELAAERGAYPLCDRHAHLDALRAGPPLP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCH
HAVCHAIERDGLRHSHLTSFAPVIGVSLAFGDHCSPGIEPARAWIEHRPVRAGAAGAPGM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
RAENHAHRLFRSLRGERAALPDYFATDADVAPGERLAMRVALQPYVDAGIDNTLTLANHY
CCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCC
SLEEVNALLFAAWRAKLKSVAIRRADLARDAQPSDDDGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]