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The map label for this gene is rspA [H]

Identifier: 76818053

GI number: 76818053

Start: 696610

End: 697818

Strand: Direct

Name: rspA [H]

Synonym: BURPS1710b_A0510

Alternate gene names: 76818053

Gene position: 696610-697818 (Clockwise)

Preceding gene: 76818707

Following gene: 76817562

Centisome position: 21.89

GC content: 68.49

Gene sequence:

>1209_bases
GTGAAAATCGAACGGCTGAACATCATCGTCACCTGCCCCGGCCGGAATTTCGTGACGGTCAAGATCGTCACCGACGAAGG
CGTCCACGGCCTCGGCGACGCGACGCTCAACGGGCGCGAGCTCGCGGTGCGCGCGTATCTGGAGGATCACGTGTTCCCGT
GCCTGATCGGCCGGGACCCGCGCGACATCGAGGACATCTGGCAATACCTGTATCGCGGCGCGTACTGGCGGCGCGGGCCC
GTGACGATGACGGCGATCGCCGCGATCGACATGGCGCTCTGGGACATCAAGGGCAAGCTCGCCGGCATGCCCGTCTATCA
GTTGCTCGGCGGCAAGAGCCGCGAAGGGCTGATGGTCTATGGCCACGCGAACGGGCGCGATCACGACGAGGCGATCGACG
CCGCGCACCGGCACATCGCCGCGGGCTACCGCGCGATCCGCGTGCAGTCGGGCGTGCCGGGGCTGGCGAAGGTGTACGGC
GTCGGCAAGACGGCGGGCGCGTACGAGCCCGCCGAAAAAGGCCTGCCGCCCGAGGAGCCGTGGGACACCGCGCTCTACCT
GCGCCATACGCCCGAGCTGTTCCGCAAGGTGCGCGACGCGCTCGGCGACGCGCCGCACCTGCTGCACGACGCGCATCACC
GGCTCACGCCGATCGAGGCGGCGCGCCTCGGCCGCGATCTCGAGCCGTACCGGCTGTTCTGGCTCGAGGACGCGACGCCC
GCCGAGAACCAGGACGCATTCCGGCTGATCCGCAGCCACACGACGACGCCGCTCGCGGTCGGCGAGGTCTTCAATTCGAT
CTGGGATTGCAAGGACCTGATCCGCGATCAGCTGATCGACTACATCCGCGCGACGATCGTGCACGCGGGCGGCATCACGC
ACGTGCGCCGCATCGCCGATTACGCGGCGATGTACCAGGTCCGCACGGGCTTTCACGGCGCGACCGACCTGTCGCCCGTG
TGCATGGCGGCCGCGGTGAACTTCGGACTGTGGGCGCCGAACTTCGGCATCCAGGAGCTGATGCCGCACACGGCGCTGAC
CGACGAGGTGTTCCCGCACAACTATCGCTTCGACGACGGCTATCTCGTGATGGACGACGTGCCCGGCCTCGGCGTCGACA
TCGACGAGACGCTCGCCGCGAACTATCCGTACGAGCGCGCCTACCTGCCCGTCGCCCGCCTGCGCGACGGCTCGATGTGG
AACTGGTGA

Upstream 100 bases:

>100_bases
GGTCGCGGTGCACGTCGACCGCGTGAACGCCCACTTGCCCGAGGTGCGCGAGCGCGCGCCCCACTACTTTGTCGACTGAC
CCCCTCACCGGAGAAACCGC

Downstream 100 bases:

>100_bases
TCGCGCGCCGCCCCACCGTCTAGACGAAACACGAAGGAGAATCAGGACATGCTGAGCGTCGTCGTCGACCAGCCGCATAG
CATGGGCGTGCGCGAAATGC

Product: starvation sensing protein RspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKGKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTPLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW

Sequences:

>Translated_402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKGKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTPLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW
>Mature_402_residues
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDPRDIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDIKGKLAGMPVYQLLGGKSREGLMVYGHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYG
VGKTAGAYEPAEKGLPPEEPWDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
AENQDAFRLIRSHTTTPLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDGYLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMW
NW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=65.8415841584158, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI48994953, Length=413, Percent_Identity=30.0242130750605, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI226510960, Length=348, Percent_Identity=24.4252873563218, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45063; Mature: 45063

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDP
CCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEHHHHCCEEEEECCCC
RDIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDIKGKLAGMPVYQLLGGKSREGLMVY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEE
GHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYGVGKTAGAYEPAEKGLPPEEP
ECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCCCCCCC
WDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCC
AENQDAFRLIRSHTTTPLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCEECCCC
YLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMWNW
EEEEECCCCCCCCHHHHHHCCCCCHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MKIERLNIIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEDHVFPCLIGRDP
CCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHEEEEEHHHHCCEEEEECCCC
RDIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDIKGKLAGMPVYQLLGGKSREGLMVY
CCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEE
GHANGRDHDEAIDAAHRHIAAGYRAIRVQSGVPGLAKVYGVGKTAGAYEPAEKGLPPEEP
ECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHCCCCCCCC
WDTALYLRHTPELFRKVRDALGDAPHLLHDAHHRLTPIEAARLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCC
AENQDAFRLIRSHTTTPLAVGEVFNSIWDCKDLIRDQLIDYIRATIVHAGGITHVRRIAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHTALTDEVFPHNYRFDDG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCEECCCC
YLVMDDVPGLGVDIDETLAANYPYERAYLPVARLRDGSMWNW
EEEEECCCCCCCCHHHHHHCCCCCHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]