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The map label for this gene is purH [H]

Identifier: 75910022

GI number: 75910022

Start: 4750459

End: 4751979

Strand: Direct

Name: purH [H]

Synonym: Ava_3818

Alternate gene names: 75910022

Gene position: 4750459-4751979 (Clockwise)

Preceding gene: 75910013

Following gene: 75910027

Centisome position: 74.63

GC content: 48.13

Gene sequence:

>1521_bases
ATGGCGCGTCTAGCACTGCTGAGTGTATCTAACAAGACTGGTTTAATTGACTTAGCTCGTCGCTTGGTAGAAGAATTTGA
GTTTGATTTAATCAGCAGTGGGGGGACAGCCCAAGCCCTCAAGGATGCGGGTTTACCTGTGACGAAGGTTGCAGATTACA
CGGGTTCGCCAGAGATTTTGGGTGGACGGGTGAAAACTCTCCATCCCCGGATTCATGGGGGGATTTTGGCTAGGCGGGAT
GTACCTAGTGATTTGACGGATTTGGAAAATAACCAAATTCGCCCGATTGATTTGGTGGTGGTGAATCTTTACCCGTTTGA
GGAGACTATTGCTAAACCAGGGGTGACGTTGGCGGAAGCTGTGGAACAAATTGATATTGGCGGCCCGGCGATGTTACGGG
CATCATCAAAGAATTTTGCCCATCTAACAGTCTTATGTGATCCGGCGCAGTATGATGAATATCTGCAAGAATTACGACAA
AATAACGGAGTAGCTTCCTTAGAATTTCGGCAAAAGGCAGCTTTGAAGGGGTTTTTGCATACGGCGAGTTATGATAGTGC
CATTGCCTCTTACCTCTCAGGTACACAACAGCATACCCTCAACGGTACAGAATTACAATCTCTGCGTTACGGTGAGAATC
CCCATCAGCCCGCAGCTTGGTATCAAACTGGAACTACGCCAACAGGGTGGACGGCAGCCAAGAAACTGCAAGGCAAGGAA
CTCAGCTACAATAATTTGGTTGACTTAGAAGCCGCCCGCCGCATTATTGCAGAGTTCACTGATACGCCAGCCGCCACGAT
TATTAAACATACTAATCCCTGCGGTACGGCATTGGCAGATACCATCGTGGAAGCTTATCAAAAAGCTTTTAATGCTGACG
CTACTTCGGCATTTGGGGGGATTGTCGCCCTGAACCGCCCTATTGATGCAGCGACAGCCAGCGAGTTAACCAAGACGTTT
TTAGAATGTGTAGTTGCGCCTGATTGCGATGCAGAAGCGCAAAAAATTCTGGCGAAGAAATCTAATGTGCGGGTGTTGAC
TTTAGCAGATTTGAGTACAGGCCCCAAAACTCTGGTAAAACAAATTGCTGGCGGTTTCCTGGTGCAGGCTGCGGATGATA
TTGCTGCTGACACAATTCAATGGCAAGTAGTTACAGAACGCCAACCTACTGCTGATGAATTAGCAGAATTGTTATTTGCA
TGGAAAGTCTGCAAACACGTTAAATCTAATGCTATTGTTGTGACAAGCGATCGCACTACTCTTGGTGTAGGTGCAGGACA
AATGAACCGCATTGGTTCAACGAAAATTGCCCTAGAACAAGCAGGGGACAAAGCCAAAGGTGCAATCCTCGCCAGCGATG
GATTTTTCCCCTTTGATGATACCGTGAGAACCGCCGCCGCCGCCGGTATTAGCGCCATTGTCCAGCCAGGGGGAAGCCTG
CGCGATCAAGATTCTGTCAAGGCTGCCAATGAACTCGGTTTGTTAATGGTGCTGACTGGGGTGCGGCATTTTTTACATTA
G

Upstream 100 bases:

>100_bases
CAGGTCGCCACTACTGTATTTGAGAATTATTCCTCATGGTCTTGAAGTGAAGCCAGGGTTGTGTGAGAATTGGGGTCTTT
GGCATCTGAGGAATTGAGAC

Downstream 100 bases:

>100_bases
GAGGTAGGGAGCAGGGGAGGCAGGGGAGGCGGGGGGAGAAATTACTCCAAGATTTTCCCCCAATCCCCATTACCCCAGTC
CCCAATCCCTCACAATGGTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MARLALLSVSNKTGLIDLARRLVEEFEFDLISSGGTAQALKDAGLPVTKVADYTGSPEILGGRVKTLHPRIHGGILARRD
VPSDLTDLENNQIRPIDLVVVNLYPFEETIAKPGVTLAEAVEQIDIGGPAMLRASSKNFAHLTVLCDPAQYDEYLQELRQ
NNGVASLEFRQKAALKGFLHTASYDSAIASYLSGTQQHTLNGTELQSLRYGENPHQPAAWYQTGTTPTGWTAAKKLQGKE
LSYNNLVDLEAARRIIAEFTDTPAATIIKHTNPCGTALADTIVEAYQKAFNADATSAFGGIVALNRPIDAATASELTKTF
LECVVAPDCDAEAQKILAKKSNVRVLTLADLSTGPKTLVKQIAGGFLVQAADDIAADTIQWQVVTERQPTADELAELLFA
WKVCKHVKSNAIVVTSDRTTLGVGAGQMNRIGSTKIALEQAGDKAKGAILASDGFFPFDDTVRTAAAAGISAIVQPGGSL
RDQDSVKAANELGLLMVLTGVRHFLH

Sequences:

>Translated_506_residues
MARLALLSVSNKTGLIDLARRLVEEFEFDLISSGGTAQALKDAGLPVTKVADYTGSPEILGGRVKTLHPRIHGGILARRD
VPSDLTDLENNQIRPIDLVVVNLYPFEETIAKPGVTLAEAVEQIDIGGPAMLRASSKNFAHLTVLCDPAQYDEYLQELRQ
NNGVASLEFRQKAALKGFLHTASYDSAIASYLSGTQQHTLNGTELQSLRYGENPHQPAAWYQTGTTPTGWTAAKKLQGKE
LSYNNLVDLEAARRIIAEFTDTPAATIIKHTNPCGTALADTIVEAYQKAFNADATSAFGGIVALNRPIDAATASELTKTF
LECVVAPDCDAEAQKILAKKSNVRVLTLADLSTGPKTLVKQIAGGFLVQAADDIAADTIQWQVVTERQPTADELAELLFA
WKVCKHVKSNAIVVTSDRTTLGVGAGQMNRIGSTKIALEQAGDKAKGAILASDGFFPFDDTVRTAAAAGISAIVQPGGSL
RDQDSVKAANELGLLMVLTGVRHFLH
>Mature_505_residues
ARLALLSVSNKTGLIDLARRLVEEFEFDLISSGGTAQALKDAGLPVTKVADYTGSPEILGGRVKTLHPRIHGGILARRDV
PSDLTDLENNQIRPIDLVVVNLYPFEETIAKPGVTLAEAVEQIDIGGPAMLRASSKNFAHLTVLCDPAQYDEYLQELRQN
NGVASLEFRQKAALKGFLHTASYDSAIASYLSGTQQHTLNGTELQSLRYGENPHQPAAWYQTGTTPTGWTAAKKLQGKEL
SYNNLVDLEAARRIIAEFTDTPAATIIKHTNPCGTALADTIVEAYQKAFNADATSAFGGIVALNRPIDAATASELTKTFL
ECVVAPDCDAEAQKILAKKSNVRVLTLADLSTGPKTLVKQIAGGFLVQAADDIAADTIQWQVVTERQPTADELAELLFAW
KVCKHVKSNAIVVTSDRTTLGVGAGQMNRIGSTKIALEQAGDKAKGAILASDGFFPFDDTVRTAAAAGISAIVQPGGSLR
DQDSVKAANELGLLMVLTGVRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=471, Percent_Identity=38.6411889596603, Blast_Score=272, Evalue=6e-73,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=46.5779467680608, Blast_Score=458, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71985564, Length=473, Percent_Identity=37.8435517970402, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=301, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323768, Length=472, Percent_Identity=39.8305084745763, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6323056, Length=469, Percent_Identity=39.2324093816631, Blast_Score=272, Evalue=7e-74,
Organism=Drosophila melanogaster, GI24649832, Length=467, Percent_Identity=39.8286937901499, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54100; Mature: 53969

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLALLSVSNKTGLIDLARRLVEEFEFDLISSGGTAQALKDAGLPVTKVADYTGSPEIL
CCEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCC
GGRVKTLHPRIHGGILARRDVPSDLTDLENNQIRPIDLVVVNLYPFEETIAKPGVTLAEA
CCCCEEECCHHHCCEEECCCCCCHHHHCCCCCCCEEEEEEEEEECCHHHHHCCCCHHHHH
VEQIDIGGPAMLRASSKNFAHLTVLCDPAQYDEYLQELRQNNGVASLEFRQKAALKGFLH
HHHHCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
TASYDSAIASYLSGTQQHTLNGTELQSLRYGENPHQPAAWYQTGTTPTGWTAAKKLQGKE
HCCHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHCCCCC
LSYNNLVDLEAARRIIAEFTDTPAATIIKHTNPCGTALADTIVEAYQKAFNADATSAFGG
CCCCCEECHHHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCCC
IVALNRPIDAATASELTKTFLECVVAPDCDAEAQKILAKKSNVRVLTLADLSTGPKTLVK
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
QIAGGFLVQAADDIAADTIQWQVVTERQPTADELAELLFAWKVCKHVKSNAIVVTSDRTT
HHHCCEEEEEHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
LGVGAGQMNRIGSTKIALEQAGDKAKGAILASDGFFPFDDTVRTAAAAGISAIVQPGGSL
EEECCCCCCCCCCCEEEHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RDQDSVKAANELGLLMVLTGVRHFLH
CCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARLALLSVSNKTGLIDLARRLVEEFEFDLISSGGTAQALKDAGLPVTKVADYTGSPEIL
CEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCHHHHCCCCCCCCCC
GGRVKTLHPRIHGGILARRDVPSDLTDLENNQIRPIDLVVVNLYPFEETIAKPGVTLAEA
CCCCEEECCHHHCCEEECCCCCCHHHHCCCCCCCEEEEEEEEEECCHHHHHCCCCHHHHH
VEQIDIGGPAMLRASSKNFAHLTVLCDPAQYDEYLQELRQNNGVASLEFRQKAALKGFLH
HHHHCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHH
TASYDSAIASYLSGTQQHTLNGTELQSLRYGENPHQPAAWYQTGTTPTGWTAAKKLQGKE
HCCHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHCCCCC
LSYNNLVDLEAARRIIAEFTDTPAATIIKHTNPCGTALADTIVEAYQKAFNADATSAFGG
CCCCCEECHHHHHHHHHHHCCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHCCC
IVALNRPIDAATASELTKTFLECVVAPDCDAEAQKILAKKSNVRVLTLADLSTGPKTLVK
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
QIAGGFLVQAADDIAADTIQWQVVTERQPTADELAELLFAWKVCKHVKSNAIVVTSDRTT
HHHCCEEEEEHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
LGVGAGQMNRIGSTKIALEQAGDKAKGAILASDGFFPFDDTVRTAAAAGISAIVQPGGSL
EEECCCCCCCCCCCEEEHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
RDQDSVKAANELGLLMVLTGVRHFLH
CCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA