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The map label for this gene is topA [H]

Identifier: 75909988

GI number: 75909988

Start: 4716879

End: 4719065

Strand: Direct

Name: topA [H]

Synonym: Ava_3784

Alternate gene names: 75909988

Gene position: 4716879-4719065 (Clockwise)

Preceding gene: 75909987

Following gene: 75909989

Centisome position: 74.1

GC content: 46.23

Gene sequence:

>2187_bases
ATGCCGAAACGCTTGCTAGTGGTTGAATCTCCGGGTAAAGTGAAAAAGCTCAGTCAGATTTTGGGTTCTGAGTGGATTGT
CCGTGCTTCTTGTGGACACATTCGGGAACTGAGTGATGAGGGTGAGGATGCTTTGGGGTTCAGCATGGATGGTGGTAGTG
TGCAGTGTCACTACGTACCGCGTGACCAACGTTCAAAAGAAACTATCCAGCAGTTAAAGAGTGCGGCGAAGCAGGTGAGT
GAAGTTGTGTTAGCGACTGACCCGGATAGGGAAGGCGAAACCATTGCTTGGCATCTCAAGGAAGTACTGGGATTGAGAGA
ACCGAAACGAGTTGTCTATACAGAGATTACAGCCTCGGCGGTACAGAGTGCGATCGCTCATCCACGCAAACTGGACTTAA
ATTTAGTCGGGGCGGGATTGTGTCGAGATTGTTTAGATAAGTTGGTCGGGTATAAGGGTAGTCCTCTGGTTTGGGCGTTA
AATAATGGGGCAAAGAGTGTGGGGAGAGTGCAGAGTTCTACACTCCACCTGATTTGTCAACGGGAGAGAGAAATTCAAAG
TTTTGTACCTCAAGATTATTGGAGTGTTTGGGTTGATTATGTTGAAGGTTTCCGCGCTTTCTATAAAGGTGCAGTCAACA
CTCGCTCAGACTCTCCAGAAACAGAAGTAGAAATTCATGATGATGCGGCGGGAAATAGCACAACTGCGCCTGAGTCTACC
CGCGTGCTGGCGGAAGCAGAAGCTAATCGTTTAGTAGAAGAAGCACGCCGTCATCCCCACCAAATTGTGCAGTATGAGGG
AAAAATTGCCAACCGTCAACCACCCCCACCTTTTATTACTTCCACCTTGCAACAAGCGGCTGGTTCTAAGTTGAAGTTCG
CGCCAGAGAAGACGATGCAGCTAGCCCAAAAGTTGTATGAGGCTGGGTTGATTACATATATGCGGACAGACTCGGTAATG
TTGAGTCCAGAGTTTTGTGAAAGTGCGCGTCAGTGGTTGGAGCAAAATGACCCCCAGAATGTACCGAAGCAGGTTGCAAG
ACATCGCAGTAGTAAGACAGCCCAACAAGGACACGAAGCGATTCGCCCAACTGATGTGTTTCGTCCTTCCGCCCAGTTGC
GAATAGAACTATCCACAGATGAGTTTAACTTGTATGTGATGATTTGGAAACGGGCGATCGCTTCCCAATGTCGTCCAGCC
CAACTACGAAAAACGGTTGTAATTACCCAGTCTGGTCAAATTCTCTGGCAAGCAAGAGGACAAGTAGTAGAATTTCTCGG
TTACACCCGTTACTGGCCTAACCTCAGCAAGGATACCCTCTTACCCACCTTGCAACAAGGGCAAATGGTCACTTTAGAAA
ATGCTGGTCACGAAAAGAAACAAACCCAACCCCCACCACGCTACAGCGAACCCAAGTTAGTGCAACTGATGGAACGTAAA
GGTATTGGTCGTCCCAGTACCTATTCTCCCACCATTGCCACCCTGAAGAAACGGGGTTATGTGGAATTGACCAAAGACAA
CTTACACCCAACAAATCTAGGGTTAGAAGTTGATACTTTCTTACAAAAAGCCCTACCGGATTTACTAGAAGCGGAATTTA
CTGCCAAAATGGAGAATGCCCTAGATGCGATCGCTGAAGGTAAACAACCTTGGCAAATATACTTAACCACTTGGAATCAG
AATTATTTTGCCCCTGCTTTAGCCAAAGCCAAAACCATAGCTGTCGATTCAGGTAATCCGACAAAAACCTTTCCCCCGCG
TCAATACGATACTAGCCGGACTCGTTGCCCTGATTGTAATAACTTTCTCAGCAAAATTCCCAGCAGTAAACTGAAGAAAA
AATACTTCCTCAAATGCACTAGCGGTTGTGAAAACACAGTCTTATTCTGGAGTGAATTTAGTAAGACTTGGCAAGCGCCC
CGAACAAAAGATGACAAGATGGCAGAAAATGGGCAAAAGAATCATCTCTCACCTGCAACGAAACTTCCTACTCAGTTGAC
AGCATACCCCTGTCCAGTATGTAAAAGACCTTTGGAGGAGTATACTTACACCAAAGATGGACAGAAAAAAACTATGCTGC
GTTGCTCTGCATCCTCATCTCGCACTGATAAAAAACATCAAGATGTGGCTTATTTCCACACTGCTAAAGGTTGGTGGAGT
CCTAAGTTTGGGGAAATAAATAAATGA

Upstream 100 bases:

>100_bases
AAAAACAACTAATATCAATTCTCCAAAATTCAGTAATAGATCCATTCCTCAGAAACCCTTGTGATATCTGATTTCCTTAC
TGTTGAATTTGGAATTGTTT

Downstream 100 bases:

>100_bases
AAGACTATCCCTCTCAACGTAAATAGGGATAGTCTAGTTTTACCAATCGTGGGGGATAAATTTTGAATATACTAGAATTT
TCTCTACTAGTTTGGCTTGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGGSVQCHYVPRDQRSKETIQQLKSAAKQVS
EVVLATDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWAL
NNGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSDSPETEVEIHDDAAGNSTTAPEST
RVLAEAEANRLVEEARRHPHQIVQYEGKIANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVM
LSPEFCESARQWLEQNDPQNVPKQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPA
QLRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQ
NYFAPALAKAKTIAVDSGNPTKTFPPRQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAP
RTKDDKMAENGQKNHLSPATKLPTQLTAYPCPVCKRPLEEYTYTKDGQKKTMLRCSASSSRTDKKHQDVAYFHTAKGWWS
PKFGEINK

Sequences:

>Translated_728_residues
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGGSVQCHYVPRDQRSKETIQQLKSAAKQVS
EVVLATDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWAL
NNGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSDSPETEVEIHDDAAGNSTTAPEST
RVLAEAEANRLVEEARRHPHQIVQYEGKIANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVM
LSPEFCESARQWLEQNDPQNVPKQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPA
QLRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQ
NYFAPALAKAKTIAVDSGNPTKTFPPRQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAP
RTKDDKMAENGQKNHLSPATKLPTQLTAYPCPVCKRPLEEYTYTKDGQKKTMLRCSASSSRTDKKHQDVAYFHTAKGWWS
PKFGEINK
>Mature_727_residues
PKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGGSVQCHYVPRDQRSKETIQQLKSAAKQVSE
VVLATDPDREGETIAWHLKEVLGLREPKRVVYTEITASAVQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALN
NGAKSVGRVQSSTLHLICQREREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSDSPETEVEIHDDAAGNSTTAPESTR
VLAEAEANRLVEEARRHPHQIVQYEGKIANRQPPPPFITSTLQQAAGSKLKFAPEKTMQLAQKLYEAGLITYMRTDSVML
SPEFCESARQWLEQNDPQNVPKQVARHRSSKTAQQGHEAIRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQ
LRKTVVITQSGQILWQARGQVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERKG
IGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENALDAIAEGKQPWQIYLTTWNQN
YFAPALAKAKTIAVDSGNPTKTFPPRQYDTSRTRCPDCNNFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPR
TKDDKMAENGQKNHLSPATKLPTQLTAYPCPVCKRPLEEYTYTKDGQKKTMLRCSASSSRTDKKHQDVAYFHTAKGWWSP
KFGEINK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=602, Percent_Identity=24.9169435215947, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI4507635, Length=576, Percent_Identity=24.3055555555556, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI1787529, Length=652, Percent_Identity=34.2024539877301, Blast_Score=357, Evalue=2e-99,
Organism=Escherichia coli, GI1788061, Length=544, Percent_Identity=25.9191176470588, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=545, Percent_Identity=25.1376146788991, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=685, Percent_Identity=22.7737226277372, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585251, Length=623, Percent_Identity=23.7560192616372, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640096, Length=574, Percent_Identity=23.6933797909408, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82174; Mature: 82043

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGGSVQCHYVP
CCCCEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEECCCCEEEEECC
RDQRSKETIQQLKSAAKQVSEVVLATDPDREGETIAWHLKEVLGLREPKRVVYTEITASA
CCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHH
VQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALNNGAKSVGRVQSSTLHLICQ
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
REREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSDSPETEVEIHDDAAGNSTTAPEST
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
RVLAEAEANRLVEEARRHPHQIVQYEGKIANRQPPPPFITSTLQQAAGSKLKFAPEKTMQ
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCHHHHHH
LAQKLYEAGLITYMRTDSVMLSPEFCESARQWLEQNDPQNVPKQVARHRSSKTAQQGHEA
HHHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQLRKTVVITQSGQILWQARG
CCCHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCHHHCEEEEEECCCCEEEHHHH
QVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
HHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHC
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENA
CCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDAIAEGKQPWQIYLTTWNQNYFAPALAKAKTIAVDSGNPTKTFPPRQYDTSRTRCPDCN
HHHHHCCCCCEEEEEEEECCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
NFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPRTKDDKMAENGQKNHLSPAT
HHHHHCCHHHHHHHHHEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHH
KLPTQLTAYPCPVCKRPLEEYTYTKDGQKKTMLRCSASSSRTDKKHQDVAYFHTAKGWWS
HCCCHHCCCCCHHHHCCHHHCCCCCCCCHHHEEEECCCCCCCCHHHCCEEEEEECCCCCC
PKFGEINK
CCCCCCCC
>Mature Secondary Structure 
PKRLLVVESPGKVKKLSQILGSEWIVRASCGHIRELSDEGEDALGFSMDGGSVQCHYVP
CCCEEEEECCCHHHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCEEEECCCCEEEEECC
RDQRSKETIQQLKSAAKQVSEVVLATDPDREGETIAWHLKEVLGLREPKRVVYTEITASA
CCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHH
VQSAIAHPRKLDLNLVGAGLCRDCLDKLVGYKGSPLVWALNNGAKSVGRVQSSTLHLICQ
HHHHHCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
REREIQSFVPQDYWSVWVDYVEGFRAFYKGAVNTRSDSPETEVEIHDDAAGNSTTAPEST
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
RVLAEAEANRLVEEARRHPHQIVQYEGKIANRQPPPPFITSTLQQAAGSKLKFAPEKTMQ
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEECCHHHHHH
LAQKLYEAGLITYMRTDSVMLSPEFCESARQWLEQNDPQNVPKQVARHRSSKTAQQGHEA
HHHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IRPTDVFRPSAQLRIELSTDEFNLYVMIWKRAIASQCRPAQLRKTVVITQSGQILWQARG
CCCHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCHHHCEEEEEECCCCEEEHHHH
QVVEFLGYTRYWPNLSKDTLLPTLQQGQMVTLENAGHEKKQTQPPPRYSEPKLVQLMERK
HHHHHHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHC
GIGRPSTYSPTIATLKKRGYVELTKDNLHPTNLGLEVDTFLQKALPDLLEAEFTAKMENA
CCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LDAIAEGKQPWQIYLTTWNQNYFAPALAKAKTIAVDSGNPTKTFPPRQYDTSRTRCPDCN
HHHHHCCCCCEEEEEEEECCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
NFLSKIPSSKLKKKYFLKCTSGCENTVLFWSEFSKTWQAPRTKDDKMAENGQKNHLSPAT
HHHHHCCHHHHHHHHHEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHH
KLPTQLTAYPCPVCKRPLEEYTYTKDGQKKTMLRCSASSSRTDKKHQDVAYFHTAKGWWS
HCCCHHCCCCCHHHHCCHHHCCCCCCCCHHHEEEECCCCCCCCHHHCCEEEEEECCCCCC
PKFGEINK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]