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The map label for this gene is rfbD [H]

Identifier: 75909561

GI number: 75909561

Start: 4174988

End: 4175878

Strand: Direct

Name: rfbD [H]

Synonym: Ava_3355

Alternate gene names: 75909561

Gene position: 4174988-4175878 (Clockwise)

Preceding gene: 75909560

Following gene: 75909562

Centisome position: 65.59

GC content: 45.57

Gene sequence:

>891_bases
ATGAATGAATCGATCCTACTAATAGGTGGTAACGGTCAAGTAGGTCAAGAAATCCACCAAATCCTTGCACCTAAATACAA
AGTTATCTCAGCCGCACGACCCAGGATAGACCTAACTCAAGCTGATAGTCTGCGTCAAATCATCCAAGAAGTCCAACCGC
AGATAATCATTAACGCTGCCGCTTACACTGCTGTAGACAAAGCCGAAACTGAACCCGAAATAGCCACAGCTATCAACGCT
ACAGCACCCCAAATTATCGCCGAAGAAAGCCAAAAACTCGGCTCATTCTTAATTCATATCTCCACAGATTACGTTTTTGA
TGGACAGCAAAGTCACCCTTACCAAGAAACAGACCCTACTAATCCCTTAAGTGTTTACGGTAAAACCAAACTAGCCGGAG
AAATAGCCATTCAACAAACCCACCCCCAGCACCTCATTCTCCGTACAGCTTGGGTTTATGGCAGTTTTGGTAAAAGTAAC
TTTGTCAAAACCATGCTACGGTTGGGTGCAGAACGCCAAGAAATTCGGGTAGTTAAAGATCAAATTGGCTCCCCTACTTG
GGCGCAAGATATCGCTGACACCATAGCCCAGGTGATACCCCAAATACCGGAAATTTCTGGGACTTACCACTACACAAACA
CTGGGGTAATTAGCTGGTATGACTTTGCCGTGGCTATTTTTGCCGAATCCCAAAAGCTAGGCTTTCCGCTCACAGTCCAA
CAAATTATTCCTATCACCACCGCCGAATATCCGACTTTAGCTCCCCGCCCTGCCTATTCTGTCCTAGCTTGTGAGAAAAT
GTCACAAGTTTTGGGAACTGCTCCCCCCCATTGGCAAGAAAGACTGCGGTTGATGCTTCAAGACTGGCTCTCCAAATATT
CTTCATTATGA

Upstream 100 bases:

>100_bases
TGGAATGATCCTGATTTAGCGATAAATTGGCCTCTCAAAGAACCACCAATTTTATCAGCTAAAGATAGCCAGGGTCAGCC
ATTGAAAAGCGCGGAAGTAT

Downstream 100 bases:

>100_bases
AAGCACTAATTCTCTCTGGCGGTAGAGGTACACGTCTACGTCCACTCACCTATACTGGAGCAAAGCAACTTGTCCCAGTT
GCGAACAAACCTATTCTATG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNESILLIGGNGQVGQEIHQILAPKYKVISAARPRIDLTQADSLRQIIQEVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPQHLILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYDFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQERLRLMLQDWLSKYSSL

Sequences:

>Translated_296_residues
MNESILLIGGNGQVGQEIHQILAPKYKVISAARPRIDLTQADSLRQIIQEVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPQHLILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYDFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQERLRLMLQDWLSKYSSL
>Mature_296_residues
MNESILLIGGNGQVGQEIHQILAPKYKVISAARPRIDLTQADSLRQIIQEVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPQHLILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYDFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQERLRLMLQDWLSKYSSL

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=31.1188811188811, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=31.1188811188811, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=44.1780821917808, Blast_Score=253, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32853; Mature: 32853

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESILLIGGNGQVGQEIHQILAPKYKVISAARPRIDLTQADSLRQIIQEVQPQIIINAA
CCCCEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEHH
AYTAVDKAETEPEIATAINATAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPT
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCCCCC
NPLSVYGKTKLAGEIAIQQTHPQHLILRTAWVYGSFGKSNFVKTMLRLGAERQEIRVVKD
CCEEECCCCHHHEEEEEECCCCHHHHEEHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
QIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYDFAVAIFAESQKLGFPLTVQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHEEEEEECCCCCCCCEEHH
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQERLRLMLQDWLSKYSSL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNESILLIGGNGQVGQEIHQILAPKYKVISAARPRIDLTQADSLRQIIQEVQPQIIINAA
CCCCEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEHH
AYTAVDKAETEPEIATAINATAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPT
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCCCCC
NPLSVYGKTKLAGEIAIQQTHPQHLILRTAWVYGSFGKSNFVKTMLRLGAERQEIRVVKD
CCEEECCCCHHHEEEEEECCCCHHHHEEHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
QIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYDFAVAIFAESQKLGFPLTVQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEHHHHEEEEEECCCCCCCCEEHH
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQERLRLMLQDWLSKYSSL
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]