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The map label for this gene is amsH [H]

Identifier: 75909494

GI number: 75909494

Start: 4100694

End: 4102172

Strand: Direct

Name: amsH [H]

Synonym: Ava_3287

Alternate gene names: 75909494

Gene position: 4100694-4102172 (Clockwise)

Preceding gene: 75909493

Following gene: 75909495

Centisome position: 64.42

GC content: 45.77

Gene sequence:

>1479_bases
ATGCTTAACACAGGTTCGCTGAAATTTCTATCACAGCCAGCTGTGGGTGCAGCTTTGCTAACTGCTGCTCATCTCGTTGT
GCCGTTTGCCAGCGTAGCTCAGGGACAACAAGTATTTCCTAATTCGCAAACAGTACCTCCTAATAACCAACGAGTATCAC
CACCAGTACAAGTAGATACAAAATACTTGTTGGGAGGCGGCGATCTAATTCGTGTAAATGTATTTGAAGTTCCCGAATAT
GGTGGTGAATACCAAATTCCTCCAGGTGGAGCTATCAACCTACCTTTAATCGGCAGTGTTTCAGTTCAAGGGTTCACGAC
AGAACAAGCAGCCGATGTGATCACTGACAGATATCGCAGGTATCTCAAACGTCCCCAGATTTCCGTCAATCTTTTATCCC
CTCGCCCCATCAATATCTTAGTTGCTGGAGAAGTCACACGCCCAGGCTCTTACACTTTGAGCTTGCAAGGCGGTGCAGGT
AATAATCCAGGGGTACAATATCCCACTGTATTGGCTGCACTCACAACAGCCCAGGGTGTTACCTTAGCAGCAGATGTCAC
TCAAGTGAAGCTGAGACGTAAAACCAGTAGTTCTACAGAGCAAATCGTGAGCCTAAATTTGAAGGAACTCATCCAAACTG
GTAGCTCTTCACAAGATATTACTTTAAGAGATGGAGATGTTATAGTCGTGCCAACTGCTACTAGCTTCAACGTAGCAGAA
GCACGTAATTTATTTGCTGCTAACTTTGCTGCTAGCCAAACAGCACCGCGCACAGTAGCCATTATTGGTCAAGTCAATCG
TCCTGGCTCATATCTTGTGAGTGCGGGGGCGACAGAAGCTCAGGCTGGTGGGACTGGGGCTAGTGGCAGTGGTTTACCCA
CGGTGACACGAGCAATTCAATTAGCTGGCGGTATTACCTCCCAAGCAGATATTACCAAGATCAAATTACGCCGACCCACA
AGGACTAATGGCGAGCAAGGTATAGACCTAAATCTTTTGGAATTATTACGTAGTGGTGATGTTAATCAAGACGTAATTGT
CCAAGATGGAGACACAATTGTCATTCCTACTGCTACTGAGGTTAACCCAGCACTAGCGACGGAATTAGCAACTACTACCT
TGTCGCCTAATACAATTCAGGTTGGTGTGGTGGGTGAAGTTAAACGCCCAGGAGCCGTAGACATTAGACCAAATAGCTCC
TTAAACCAAGCACTACTCGCGGCTGGTGGATTTAATGACGCTAGAGCGCGTAGCGCCAGTGTGGAGTTGATTCGTCTCAA
CCCCAATGGTTCAGTGACAAAGCGTGAAGTAAAAGTTGACTTCAACCAAGGAATTAACGAAGAGACTAATCCCATACTCC
GGAATAATGATGTTGTGGTAGTTGGTCGCTCTAATATAGCTAGAACGGGTGATACGCTGAATATAGTAGCCGGCCCTCTT
GGTATAATCTTTAACTTCTTAAGATTTTTCGGACTTTAA

Upstream 100 bases:

>100_bases
GGAAAATGAGAATTGTCGGGAAACTTCAATACTTAAGTATAGTCAAGATTAAAATGTTTAGACAATACTTTACCGATGGT
AGTCTCAGAGTTTAAAAAAT

Downstream 100 bases:

>100_bases
TCTTTCTCTGGGGACGTAAGTAATTAGTTTCGTCCCCGGATTTCCTTTGAATTTATCAGAGCAGTTGGGCGATAGTGCGA
TCGCCATTTATTTTTTAATA

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLNTGSLKFLSQPAVGAALLTAAHLVVPFASVAQGQQVFPNSQTVPPNNQRVSPPVQVDTKYLLGGGDLIRVNVFEVPEY
GGEYQIPPGGAINLPLIGSVSVQGFTTEQAADVITDRYRRYLKRPQISVNLLSPRPINILVAGEVTRPGSYTLSLQGGAG
NNPGVQYPTVLAALTTAQGVTLAADVTQVKLRRKTSSSTEQIVSLNLKELIQTGSSSQDITLRDGDVIVVPTATSFNVAE
ARNLFAANFAASQTAPRTVAIIGQVNRPGSYLVSAGATEAQAGGTGASGSGLPTVTRAIQLAGGITSQADITKIKLRRPT
RTNGEQGIDLNLLELLRSGDVNQDVIVQDGDTIVIPTATEVNPALATELATTTLSPNTIQVGVVGEVKRPGAVDIRPNSS
LNQALLAAGGFNDARARSASVELIRLNPNGSVTKREVKVDFNQGINEETNPILRNNDVVVVGRSNIARTGDTLNIVAGPL
GIIFNFLRFFGL

Sequences:

>Translated_492_residues
MLNTGSLKFLSQPAVGAALLTAAHLVVPFASVAQGQQVFPNSQTVPPNNQRVSPPVQVDTKYLLGGGDLIRVNVFEVPEY
GGEYQIPPGGAINLPLIGSVSVQGFTTEQAADVITDRYRRYLKRPQISVNLLSPRPINILVAGEVTRPGSYTLSLQGGAG
NNPGVQYPTVLAALTTAQGVTLAADVTQVKLRRKTSSSTEQIVSLNLKELIQTGSSSQDITLRDGDVIVVPTATSFNVAE
ARNLFAANFAASQTAPRTVAIIGQVNRPGSYLVSAGATEAQAGGTGASGSGLPTVTRAIQLAGGITSQADITKIKLRRPT
RTNGEQGIDLNLLELLRSGDVNQDVIVQDGDTIVIPTATEVNPALATELATTTLSPNTIQVGVVGEVKRPGAVDIRPNSS
LNQALLAAGGFNDARARSASVELIRLNPNGSVTKREVKVDFNQGINEETNPILRNNDVVVVGRSNIARTGDTLNIVAGPL
GIIFNFLRFFGL
>Mature_492_residues
MLNTGSLKFLSQPAVGAALLTAAHLVVPFASVAQGQQVFPNSQTVPPNNQRVSPPVQVDTKYLLGGGDLIRVNVFEVPEY
GGEYQIPPGGAINLPLIGSVSVQGFTTEQAADVITDRYRRYLKRPQISVNLLSPRPINILVAGEVTRPGSYTLSLQGGAG
NNPGVQYPTVLAALTTAQGVTLAADVTQVKLRRKTSSSTEQIVSLNLKELIQTGSSSQDITLRDGDVIVVPTATSFNVAE
ARNLFAANFAASQTAPRTVAIIGQVNRPGSYLVSAGATEAQAGGTGASGSGLPTVTRAIQLAGGITSQADITKIKLRRPT
RTNGEQGIDLNLLELLRSGDVNQDVIVQDGDTIVIPTATEVNPALATELATTTLSPNTIQVGVVGEVKRPGAVDIRPNSS
LNQALLAAGGFNDARARSASVELIRLNPNGSVTKREVKVDFNQGINEETNPILRNNDVVVVGRSNIARTGDTLNIVAGPL
GIIFNFLRFFGL

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=189, Percent_Identity=30.6878306878307, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787218, Length=209, Percent_Identity=27.7511961722488, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 51635; Mature: 51635

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTGSLKFLSQPAVGAALLTAAHLVVPFASVAQGQQVFPNSQTVPPNNQRVSPPVQVDT
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCEECC
KYLLGGGDLIRVNVFEVPEYGGEYQIPPGGAINLPLIGSVSVQGFTTEQAADVITDRYRR
EEEECCCCEEEEEEEECCCCCCEEECCCCCEECCCEEECEEECCCCHHHHHHHHHHHHHH
YLKRPQISVNLLSPRPINILVAGEVTRPGSYTLSLQGGAGNNPGVQYPTVLAALTTAQGV
HHCCCCEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCE
TLAADVTQVKLRRKTSSSTEQIVSLNLKELIQTGSSSQDITLRDGDVIVVPTATSFNVAE
EEEECHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHH
ARNLFAANFAASQTAPRTVAIIGQVNRPGSYLVSAGATEAQAGGTGASGSGLPTVTRAIQ
HHHHEEECCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
LAGGITSQADITKIKLRRPTRTNGEQGIDLNLLELLRSGDVNQDVIVQDGDTIVIPTATE
HHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
VNPALATELATTTLSPNTIQVGVVGEVKRPGAVDIRPNSSLNQALLAAGGFNDARARSAS
CCHHHHHHHHHEECCCCEEEEEEEECCCCCCEEEECCCCCCCHHHEECCCCCHHHCCCCE
VELIRLNPNGSVTKREVKVDFNQGINEETNPILRNNDVVVVGRSNIARTGDTLNIVAGPL
EEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCEEEEEHHHH
GIIFNFLRFFGL
HHHHHHHHHHCC
>Mature Secondary Structure
MLNTGSLKFLSQPAVGAALLTAAHLVVPFASVAQGQQVFPNSQTVPPNNQRVSPPVQVDT
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCEECC
KYLLGGGDLIRVNVFEVPEYGGEYQIPPGGAINLPLIGSVSVQGFTTEQAADVITDRYRR
EEEECCCCEEEEEEEECCCCCCEEECCCCCEECCCEEECEEECCCCHHHHHHHHHHHHHH
YLKRPQISVNLLSPRPINILVAGEVTRPGSYTLSLQGGAGNNPGVQYPTVLAALTTAQGV
HHCCCCEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCE
TLAADVTQVKLRRKTSSSTEQIVSLNLKELIQTGSSSQDITLRDGDVIVVPTATSFNVAE
EEEECHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHH
ARNLFAANFAASQTAPRTVAIIGQVNRPGSYLVSAGATEAQAGGTGASGSGLPTVTRAIQ
HHHHEEECCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
LAGGITSQADITKIKLRRPTRTNGEQGIDLNLLELLRSGDVNQDVIVQDGDTIVIPTATE
HHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
VNPALATELATTTLSPNTIQVGVVGEVKRPGAVDIRPNSSLNQALLAAGGFNDARARSAS
CCHHHHHHHHHEECCCCEEEEEEEECCCCCCEEEECCCCCCCHHHEECCCCCHHHCCCCE
VELIRLNPNGSVTKREVKVDFNQGINEETNPILRNNDVVVVGRSNIARTGDTLNIVAGPL
EEEEEECCCCCEEEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCCCCCCEEEEEHHHH
GIIFNFLRFFGL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]