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The map label for this gene is yibK [C]

Identifier: 75909401

GI number: 75909401

Start: 3968996

End: 3969457

Strand: Direct

Name: yibK [C]

Synonym: Ava_3194

Alternate gene names: 75909401

Gene position: 3968996-3969457 (Clockwise)

Preceding gene: 75909400

Following gene: 75909402

Centisome position: 62.35

GC content: 43.51

Gene sequence:

>462_bases
ATGCCTCAGGTAGTTTTAGTTAATCCGCAAATACCCCCTAATACGGGCAATATTGCTCGTACTTGTGCTGCTACAGGTAC
AGAGTTACATTTAGTAGGACCATTGGGATTTGAAATTAGCGATCGCTACCTCAAAAGAGCAGGTTTAGACTACTGGCCTT
ACGTTAAACTGCACTACCACAAAACCATAGAAGCCTTTGAAAATGTACATCAAGAGCGTGGCGGTAGATTGCTAGGTTTT
TCCGTTAGCGGCAGTTCTAGTTATATTCAGTTTCCATATCAAGCTGATGATTGGTTACTATTCGGTAGTGAAACCACAGG
CTTACCTCCCACCGTCCTCTCAGCTTGTGATGCCACCTTATACATTCCTATGGCTGAACCAGGTGTCCGCAGCTTAAATC
TTTCTGTTAGCGTGGCAGTAGGCTTATTTGAAGTCCGTCGTCAGTTAGGATATCTACTATAA

Upstream 100 bases:

>100_bases
TAATAGAGAATTATTAACAAAACCTATGGTTGCCCTCTGTCTACTGGTAGAAATTGCTTAAATAATATATATTTTTATAC
AAACAGAAATTATACGGACA

Downstream 100 bases:

>100_bases
TCAGTTACAAATTTTATTTAAGCAATAATACTTATAGACAAACCATTATACAGATAATCTGCAAGGGTTTGAAGAAAATC
ATAACTATGTTATCAATTTA

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPQVVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFEISDRYLKRAGLDYWPYVKLHYHKTIEAFENVHQERGGRLLGF
SVSGSSSYIQFPYQADDWLLFGSETTGLPPTVLSACDATLYIPMAEPGVRSLNLSVSVAVGLFEVRRQLGYLL

Sequences:

>Translated_153_residues
MPQVVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFEISDRYLKRAGLDYWPYVKLHYHKTIEAFENVHQERGGRLLGF
SVSGSSSYIQFPYQADDWLLFGSETTGLPPTVLSACDATLYIPMAEPGVRSLNLSVSVAVGLFEVRRQLGYLL
>Mature_152_residues
PQVVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFEISDRYLKRAGLDYWPYVKLHYHKTIEAFENVHQERGGRLLGFS
VSGSSSYIQFPYQADDWLLFGSETTGLPPTVLSACDATLYIPMAEPGVRSLNLSVSVAVGLFEVRRQLGYLL

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=43.7908496732026, Blast_Score=127, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16854; Mature: 16723

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFEISDRYLKRAGLDYWPYVKLHYH
CCCEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEH
KTIEAFENVHQERGGRLLGFSVSGSSSYIQFPYQADDWLLFGSETTGLPPTVLSACDATL
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCHHHHHHCCCEE
YIPMAEPGVRSLNLSVSVAVGLFEVRRQLGYLL
EEECCCCCCEEEEEEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQVVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFEISDRYLKRAGLDYWPYVKLHYH
CCEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEH
KTIEAFENVHQERGGRLLGFSVSGSSSYIQFPYQADDWLLFGSETTGLPPTVLSACDATL
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCHHHHHHCCCEE
YIPMAEPGVRSLNLSVSVAVGLFEVRRQLGYLL
EEECCCCCCEEEEEEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]