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The map label for this gene is cobD [H]

Identifier: 75909334

GI number: 75909334

Start: 3882786

End: 3883748

Strand: Direct

Name: cobD [H]

Synonym: Ava_3127

Alternate gene names: 75909334

Gene position: 3882786-3883748 (Clockwise)

Preceding gene: 75909333

Following gene: 75909335

Centisome position: 61.0

GC content: 43.93

Gene sequence:

>963_bases
ATGACCAACAACATCTATGTCTTAATCGTTGCTGTAATTTTAGATTATTTAATAGGTGATCCTTGGGGTTGGCCTCATCC
GGTGCAAATTATGGGATGGGTAATTTCGTGGTTGAGCAAAATTTTTCTGCAACTGTGTCAAAAATCTCTAACACAACGCC
TAGCTGGGATTGTTCTCGCAATAATTTTGATTGTTGGTAGCGGTGGGGTTGGTTGGTTAATCGTGCAAATGACGAGATGG
CTAAATCCTGTTTTGGCGATCGCACTAGAAAGTATCATGTTAGCTAGTTGTTTTGCTTTAAAAAGTCTAGGCAAAGCAGC
TATTGATGTTTTACGACCTTTAAAAGCAGGGGATTTAACACTAGCTCGGCGGATTTTGAGTAATTATGTTGGTCGAGATA
CAGACAACCTACCAGAATCAGAAATCCTCCGAGCAGTTTTAGAAACAGTTACAGAGAATGCTACAGATGGGGTGATGGCT
CCATTGTTTTACGCTATTGTCGGTATCTGCATTCCCCATGTGGGGCCAGTTCCTCTGGCTTTGGCATATAAAGCCAGCAG
CACCCTCGATTCGATGGTGGGTTACAAAGAAGCACCATATACATATTTAGGATGGTTCAGCGCGCGACTAGAAGATTACT
TAACTTGGGTACCTTGTCGCTTGACAGTGATAACTTTAGGATTATTATCAGGGAAGCCTGGGGATGTGTGGCGAATTTGC
CGCCGTGATGCAATTAATGATCCTAGTCCCAACTCAGGGTGGAGTGAATGCGCCTATGCAGCCATTTTGGGTGTGCAGAT
GGGGGGAACAAATTGGTATCGTGGAATAGCCAAGCAAAAACCGCTTTTAGGAGATCCCACTTCTCCCATCAACCCCCAAC
ACATTCAAACAGCTTTGCAACTAACTAGATATAGCTTTTTGCTCTGGCTAGGTGTGGCGATCGCTATACTCCTGATACGC
TAA

Upstream 100 bases:

>100_bases
TTTGTATTACGATGCTCGTAGCTGGCAAGCTTCTCTTGGAGAAGAAGCAAATTTTGTGGTTTAACAATCGTCAGTTGTCG
GTTGACATTTGCTTTCATTC

Downstream 100 bases:

>100_bases
AGGTATGCGGGAGAAGAAGTAGGGGAAGCAAAAGGAAAACTTGCTCAAACATTTTCCCCCTTTCCCCCTACCTCAACTCC
TAACTTCCCCCACTCCCTAT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTNNIYVLIVAVILDYLIGDPWGWPHPVQIMGWVISWLSKIFLQLCQKSLTQRLAGIVLAIILIVGSGGVGWLIVQMTRW
LNPVLAIALESIMLASCFALKSLGKAAIDVLRPLKAGDLTLARRILSNYVGRDTDNLPESEILRAVLETVTENATDGVMA
PLFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWVPCRLTVITLGLLSGKPGDVWRIC
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYRGIAKQKPLLGDPTSPINPQHIQTALQLTRYSFLLWLGVAIAILLIR

Sequences:

>Translated_320_residues
MTNNIYVLIVAVILDYLIGDPWGWPHPVQIMGWVISWLSKIFLQLCQKSLTQRLAGIVLAIILIVGSGGVGWLIVQMTRW
LNPVLAIALESIMLASCFALKSLGKAAIDVLRPLKAGDLTLARRILSNYVGRDTDNLPESEILRAVLETVTENATDGVMA
PLFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWVPCRLTVITLGLLSGKPGDVWRIC
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYRGIAKQKPLLGDPTSPINPQHIQTALQLTRYSFLLWLGVAIAILLIR
>Mature_319_residues
TNNIYVLIVAVILDYLIGDPWGWPHPVQIMGWVISWLSKIFLQLCQKSLTQRLAGIVLAIILIVGSGGVGWLIVQMTRWL
NPVLAIALESIMLASCFALKSLGKAAIDVLRPLKAGDLTLARRILSNYVGRDTDNLPESEILRAVLETVTENATDGVMAP
LFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWVPCRLTVITLGLLSGKPGDVWRICR
RDAINDPSPNSGWSECAYAAILGVQMGGTNWYRGIAKQKPLLGDPTSPINPQHIQTALQLTRYSFLLWLGVAIAILLIR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35143; Mature: 35012

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNIYVLIVAVILDYLIGDPWGWPHPVQIMGWVISWLSKIFLQLCQKSLTQRLAGIVLA
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILIVGSGGVGWLIVQMTRWLNPVLAIALESIMLASCFALKSLGKAAIDVLRPLKAGDLT
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LARRILSNYVGRDTDNLPESEILRAVLETVTENATDGVMAPLFYAIVGICIPHVGPVPLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
LAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWVPCRLTVITLGLLSGKPGDVWRIC
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYRGIAKQKPLLGDPTSPINPQHIQTALQ
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LTRYSFLLWLGVAIAILLIR
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNNIYVLIVAVILDYLIGDPWGWPHPVQIMGWVISWLSKIFLQLCQKSLTQRLAGIVLA
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILIVGSGGVGWLIVQMTRWLNPVLAIALESIMLASCFALKSLGKAAIDVLRPLKAGDLT
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LARRILSNYVGRDTDNLPESEILRAVLETVTENATDGVMAPLFYAIVGICIPHVGPVPLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
LAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWVPCRLTVITLGLLSGKPGDVWRIC
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYRGIAKQKPLLGDPTSPINPQHIQTALQ
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LTRYSFLLWLGVAIAILLIR
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]