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The map label for this gene is dapF [H]

Identifier: 75908333

GI number: 75908333

Start: 2613173

End: 2614030

Strand: Direct

Name: dapF [H]

Synonym: Ava_2112

Alternate gene names: 75908333

Gene position: 2613173-2614030 (Clockwise)

Preceding gene: 75908332

Following gene: 75908334

Centisome position: 41.05

GC content: 37.88

Gene sequence:

>858_bases
ATGAAGTTCTATAAATATCATGCTCTTGGTAACGATTATTTAGTTATCAATCCTCAGGATTTAAAATTTAGCCTAACGCC
TGAAAAAATTAAAATAATTTGCCATCGAAATTTTGGTATTGGATCTGATGGTATTTTGTTGGGGCCTTTAGCATCAACAA
AAGCACAATTCGCCTTACGCATTTTCAACCCCGACGGAAGTGAGGCAGAAAAAAGCGGTAATGGACTGCGGATTTTCTCC
CGTTATTTATGGGATATGGGACTAGTTGATGAAAATCCATTCTCAATTGAAACTGTAGGTGGACTAGTGGAATCTGCGAT
CAAAGATGCAGGTAAAACAGTCCAAGTAGAGATGGGGAAAGTCAGCTTTTGGAGTCGTGATATTCCAGTTGTTGGCGATG
ACAGAGAAGTAATTCAAGAAAAAATATTTCTTGATGAAGGCATTTTTACATTTTGTGCAGCCACAATAGGTAATCCACAC
TGTGTAATTCTTTTAGATGACATCAATGCCGATGTTGCCAGAAATTTTGGCCCAATATTTGAAGTTTTTCCTCTTTTCCC
CAATCGCACTAATGTGCAGTTTATGAAGGTTTTAGATAGAAGCACTATTCAAATTGAAATTTGGGAAAGAGGAGCAGGTT
ACACTTTAGCTTCAGGTAGTAGTAGTAGCGCGGCGGCGGCTACTGCACATAAACTTGGTTTGTGTGACTCTGAAATTATT
GTCCAAATGCCTGGGGGAGACATTTTAATTCAAATTAAAGATGACTTTCATATCTCTATGACTGGTTTTGTCACAAAAGT
TGCCCAAGGAGAATTGTCAACAGAAATATTTGCCATAGAAACTGTCAGTAATATCTGA

Upstream 100 bases:

>100_bases
CTCAAGATAAGCAAACAGAAGTAACAGCATAAAACTTAAAATAAACCCAACTCACTACAAGTTGGGTTTATTTATTTCTC
CTAATTATTTACTTCAACGA

Downstream 100 bases:

>100_bases
GAAAATTACTGAACCAATATCTAGATAGTAGAGTGTAAATTCAAAAATATTGCATCTACTACCTTCATGAATCCTCACAA
AATAAAGGCTTGGGAAGAAG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKFYKYHALGNDYLVINPQDLKFSLTPEKIKIICHRNFGIGSDGILLGPLASTKAQFALRIFNPDGSEAEKSGNGLRIFS
RYLWDMGLVDENPFSIETVGGLVESAIKDAGKTVQVEMGKVSFWSRDIPVVGDDREVIQEKIFLDEGIFTFCAATIGNPH
CVILLDDINADVARNFGPIFEVFPLFPNRTNVQFMKVLDRSTIQIEIWERGAGYTLASGSSSSAAAATAHKLGLCDSEII
VQMPGGDILIQIKDDFHISMTGFVTKVAQGELSTEIFAIETVSNI

Sequences:

>Translated_285_residues
MKFYKYHALGNDYLVINPQDLKFSLTPEKIKIICHRNFGIGSDGILLGPLASTKAQFALRIFNPDGSEAEKSGNGLRIFS
RYLWDMGLVDENPFSIETVGGLVESAIKDAGKTVQVEMGKVSFWSRDIPVVGDDREVIQEKIFLDEGIFTFCAATIGNPH
CVILLDDINADVARNFGPIFEVFPLFPNRTNVQFMKVLDRSTIQIEIWERGAGYTLASGSSSSAAAATAHKLGLCDSEII
VQMPGGDILIQIKDDFHISMTGFVTKVAQGELSTEIFAIETVSNI
>Mature_285_residues
MKFYKYHALGNDYLVINPQDLKFSLTPEKIKIICHRNFGIGSDGILLGPLASTKAQFALRIFNPDGSEAEKSGNGLRIFS
RYLWDMGLVDENPFSIETVGGLVESAIKDAGKTVQVEMGKVSFWSRDIPVVGDDREVIQEKIFLDEGIFTFCAATIGNPH
CVILLDDINADVARNFGPIFEVFPLFPNRTNVQFMKVLDRSTIQIEIWERGAGYTLASGSSSSAAAATAHKLGLCDSEII
VQMPGGDILIQIKDDFHISMTGFVTKVAQGELSTEIFAIETVSNI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=34.9090909090909, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31304; Mature: 31304

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYKYHALGNDYLVINPQDLKFSLTPEKIKIICHRNFGIGSDGILLGPLASTKAQFALR
CCEEEEEECCCCEEEECCCCCEEEECCCCEEEEEECCCCCCCCCEEEECCCCCCEEEEEE
IFNPDGSEAEKSGNGLRIFSRYLWDMGLVDENPFSIETVGGLVESAIKDAGKTVQVEMGK
EECCCCCHHHHCCCCEEEHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECE
VSFWSRDIPVVGDDREVIQEKIFLDEGIFTFCAATIGNPHCVILLDDINADVARNFGPIF
EEEECCCCCEECCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCE
EVFPLFPNRTNVQFMKVLDRSTIQIEIWERGAGYTLASGSSSSAAAATAHKLGLCDSEII
EEEECCCCCCCCHHEEECCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEE
VQMPGGDILIQIKDDFHISMTGFVTKVAQGELSTEIFAIETVSNI
EEECCCEEEEEECCCEEEEEHHHHHHHHCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MKFYKYHALGNDYLVINPQDLKFSLTPEKIKIICHRNFGIGSDGILLGPLASTKAQFALR
CCEEEEEECCCCEEEECCCCCEEEECCCCEEEEEECCCCCCCCCEEEECCCCCCEEEEEE
IFNPDGSEAEKSGNGLRIFSRYLWDMGLVDENPFSIETVGGLVESAIKDAGKTVQVEMGK
EECCCCCHHHHCCCCEEEHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEECE
VSFWSRDIPVVGDDREVIQEKIFLDEGIFTFCAATIGNPHCVILLDDINADVARNFGPIF
EEEECCCCCEECCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCE
EVFPLFPNRTNVQFMKVLDRSTIQIEIWERGAGYTLASGSSSSAAAATAHKLGLCDSEII
EEEECCCCCCCCHHEEECCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEE
VQMPGGDILIQIKDDFHISMTGFVTKVAQGELSTEIFAIETVSNI
EEECCCEEEEEECCCEEEEEHHHHHHHHCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9139910; 11759840 [H]